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Journals / Hacettepe Journal of Biology and Chemistry / 2023 / Cilt: 51 Sayı: 4

COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS

Ece BATTALOĞLU
Zehra Gül MORÇİMEN
Aylin ŞENDEMİR
Pages
327–340
Publication Date
2023-11-07
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was found that 5µg/mL LPS concentration induced gliosis and hyaluronic acid-coated PCL scaffolds have shown a protective effect on gliosis.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Gliosis is a condition that plays a negative role in various neurological pathologies.
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Key Findings & Data:First, in vitro gliosis model was obtained by inducing U-87 MG cells with LPS and IFN-γ.
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Conclusion & Impact:It was found that 5µg/mL LPS concentration induced gliosis and hyaluronic acid-coated PCL scaffolds have shown a protective effect on gliosis.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Gliosis · neural tissue engineering · electrospinning

Abstract

Gliosis is a condition that plays a negative role in various neurological pathologies. In this study, it was aimed to obtain and compare the glio-protective effects of electrospun fibrous scaffolds coated by different biopolymers. First, in vitro gliosis model was obtained by inducing U-87 MG cells with LPS and IFN-γ. The optimized model was applied to U-87 MG cells grown on polycaprolactone (PCL), hyaluronic acid (HA) coated, gelatin coated, and collagen coated PCL scaffolds. Immunofluorescent (IF) staining was performed with glial fibrillary acidic protein (GFAP) antibody to determine the level of gliosis. It was found that 5µg/mL LPS concentration induced gliosis and hyaluronic acid-coated PCL scaffolds have shown a protective effect on gliosis.

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Cite This Article

BATTALOĞLU, E., MORÇİMEN, Z. G., ŞENDEMİR, A. (2023). COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS. Hacettepe Journal of Biology and Chemistry, Cilt: 51 Sayı: 4, 327–340. https://ajindex.com/articles/1016041

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References (8)

  1. R. Huang, Y. Zhang, B. Han, Y. Bai, R. Zhou, G. Gan, J. Chao, H. Yao, Circular RNA HIPK2 regulates astrocyte activation via cooperation of autophagy and ER stress by targeting MIR124-2HG, Autophagy, 13 (2017).
  2. Y. Hu, H. Zhang, H. Wei, H. Cheng, J. Cai, X. Chen, L. Xia, H. Wang, R. Chai, Scaffolds with anisotropic structure for neural tissue engineering, Engineered Regeneration, 3 (2022) 154–162.
  3. R.A.L. De Sousa, Reactive gliosis in Alzheimer’s disease: a crucial role for cognitive impairment and memory loss, Metab Brain Dis, 37 (2022) 851–857. M. Motavaf, • Majid Sadeghizadeh, • Mohammad Javan, Attempts to Overcome Remyelination Failure: Toward Opening New Therapeutic Avenues for Multiple Sclerosis, Cell Mol Neurobiol, 37 (n.d.). S.C. Yetis, D.A. Ekinci, E. Cakir, E.M. Eksioglu, U.E. Ayten, A. Capar, B.U. Toreyin, B.E. Kerman, Myelin segmentation in fluorescence microscopy images, TIPTEKNO 2019 - Tip Teknolojileri Kongresi, (2019).
  4. H. Wang, G. Song, H. Chuang, C. Chiu, A. Abdelmaksoud, Y. Ye, L. Zhao, Portrait of glial scar in neurological diseases, Int J Immunopathol Pharmacol, 31 (2018) 1–6.
  5. Y. Wang, H. Tan, X. Hui, Biomaterial Scaffolds in Regenerative Therapy of the Central Nervous System, (2018).
  6. Q. Zhang, B. Shi, J. Ding, L. Yan, J.P. Thawani, C. Fu, X. Chen, Polymer scaffolds facilitate spinal cord injury repair, Acta Biomater, 88 (2019) 57–77.
  7. H. Yin, T. Jiang, X. Deng, M. Yu, H. Xing, X. Ren, A cellular spinal cord scaffold seeded with rat adipose-derived stem cells facilitates functional recovery via enhancing axon regeneration in spinal cord injured rats, Mol Med Rep, 17 (2018) 2998. M. Li, H. Hu, Z. Yu, Y. Ding, Y. Wang, M. Wu, W. Qu, B. Chen, W. Shu, H. Tian, X. Ou, X. Zhang, Polymer-Based Scaffold Strategies for Spinal Cord Repair and Regeneration, (2020). Z. Álvarez, A.N. Kolberg-Edelbrock, I.R. Sasselli, J.A. Ortega, R. Qiu, Z. Syrgiannis, P.A. Mirau, F. Chen, S.M. Chin, S. Weigand, E. Kiskinis, S.I. Stupp, Bioactive scaffolds with enhanced supramolecular motion promote recovery from spinal cord injury, Science (1979), 374 (2021) 848–856.
  8. J.-Z. Yeh, D.-H. Wang, J.-H. Cherng, Y.-W. Wang, G.-Y. Fan, N.-H. Liou, J.-C. Liu, C.-H. Chou, A Collagen-Based Scaffold for Promoting Neural Plasticity in a Rat Model of Spinal Cord Injury, (n.d.). Y. Liu, H. Ye, K. Satkunendrarajah, G.S. Yao, Y. Bayon, M.G. Fehlings, A self-assembling peptide reduces glial scarring, attenuates post-traumatic inflammation and promotes neurological recovery following spina