ASETİLSALİSİLİK ASİT ve VİTAMİN C KOMBİNASYONUNUN SIĞIR NÖTROFİL FONKSİYONLARINA ETKİLERİNİN İN VİTRO ŞARTLARDA İNCELENMESİ

Investigation of The Effects of Combination of Acetylsalicylic Acid and Vitamin C On Functions of Bovine Neutrophils in Vitro Conditions

Ahmet Levent Baş, Nuri Başpınar, Muammer ELMAS, Seyfullah HALİLOĞLU, Enver YAZAR

Özet

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Abstract

In Ihis study, the effects ol combination of acetylsalicylic acid (ASA) and vitamin C at dilferenl doses on lunctions of bovine neuırophils (polymorphonuclear leucocytelPMNl), and the elfacts of dillerent ASA concenırations on accumulation of vitamin C in neutrophils were investigated. Neutrophils were obtained from six heatthy Holstein· heifers in University Dairy Herd. Phagocytic and microbicidal activity of PMNl were measured with fluorescent mic· roscopic technlque and intracellular vitamin C levels were detenııined by spectrophotometer. Inlracellular vitamin C le· vels were incerased by combination of ASA and vitamin C al different corıcentrations, bul this increase was not sta· tistically significanl. Furthenııore, phagocytic and microbicidal activity of PMNl were not statistically changed by these combinations

Anahtar kelimeler: asetilsalisilik asit, vitamin C, nötrofil fonksiyonları, sığır