Streptozotosin ile Oluşturulan Diyabette Testiküler Oksidatif Stres ve Apoptozun Hafifletilmesinde Kuersetin’in Rolü

The Role of Quercetin in Alleviating the Testicular Oxidative Stress and Apoptosis in Streptozotocin-Induced Diabetes

Hafize UZUN, Matem TUNÇDEMİR, Ramila HAJIYEVA, Sinem DURMUŞ, Remise GELİŞGEN, Volkan SÖZER, Eda BÜYÜKÇOLPAN MİRZATAŞ, Gönül ŞİMŞEK

Özet

Amaç: Bu çalışmada, streptozotosin ile diyabet oluşturulan erkek sıçanlarda testis dokusunda kuersetin’in oksidatif stres ve apoptoz üzerindeki etkilerini araştırmak amaçlanmıştır. Yöntemler: Çalışma kapsamında 32 erkek Wistar Albino sıçan 4 gruba ayrıldı. Grup 1: Kontrol grubu (30 gün boyunca sadece intraperitoneal %0,9 sodyum klorür uygulandı, n = 8), grup 2: Diyabet grubu (DM grubu, %0,9 sodyum klorür çözeltisinde taze olarak hazırlanmış 50 mg/kg streptozotosinin intraperitoneal olarak enjeksiyonu ile oluşturuldu, n = 8), grup 3: Diyabet + Kuersetin grubu (DM+QR grubu, Tek doz 50 mg/kg streptozotosin intraperitoneal enjekte edildi ve 30 gün boyunca %4 etanol çözeltisinde seyreltilmiş 20 mg/kg/gün kuersetin intraperitoneal uygulandı, n = 8), grup 4: Kontrol + Kuersetin grubu (Kontrol +QR grubu, 30 gün boyunca 20 mg/kg/gün kuersetin intraperitoneal uygulandı, n = 8). Çalışmanın 31. gününde anestezi altında testis dokusu örnekleri alındı. Malondialdehit

Abstract

Objective: The aim of this study was to investigate the effects of quercetin on oxidative stress and apoptosis in testicular tissue of streptozotocin-induced diabetic male rats. Methods: Thirty-two Wistar type Albino rats were divided into 4 groups. The groups are planned as follows: group 1: control group (only 0.9% sodium chloride, i.p. injection for 30 days, n = 8), group 2: DM group (streptozotocin: 50 mg/kg, freshly dissolved in 0.9% sodium chloride, i.p, n = 8), group 3: DM + QR group (quercetin: 20 mg/kg/day, diluted in 4% ethanol, 30 days, i.p.) (streptozotocin: 50 mg/kg, i.p., single dose, n = 8), group 4: control + QR group (quercetin: 20 mg/kg/day, 30 day, i.p., n = 8). Testicular tissue samples were taken under anesthesia on the 31st day of the study. Malondialdehyde, lipid hydroperoxide, advanced oxidation protein products, total thiol, nonprotein sulfhydryl compounds, and Cu,Zn-superoxide dismutase were determined by spectrophotometric method. Results: Malondialdehyde and lipid hydroperoxide levels were higher in the DM group compared to the control and DM+ QR group. Advanced oxidation protein product levels were lower in the control group than in the control+ QR and DM group, while total thiol, nonprotein sulfhydryl levels, and Cu,Zn-superoxide dismutase activity were higher in DM+ QR group compared to control and DM groups. In the DM+ QR group, a significant decrease was found in the number of apoptotic cells compared to the DM group (P < .001). Conclusion: Quercetin administration can be potentially beneficial and effective in preventing oxidative stress (lipid and protein) damage and apoptosis in the testicles of diabetic rats.