The Role of Quercetin in Alleviating the Testicular Oxidative Stress and Apoptosis in Streptozotocin-Induced Diabetes

Streptozotosin ile Oluşturulan Diyabette Testiküler Oksidatif Stres ve Apoptozun Hafifletilmesinde Kuersetin’in Rolü

Hafize UZUN, Matem TUNÇDEMİR, Ramila HAJIYEVA, Sinem DURMUŞ, Remise GELİŞGEN, Volkan SÖZER, Eda BÜYÜKÇOLPAN MİRZATAŞ, Gönül ŞİMŞEK

Abstract

Objective: The aim of this study was to investigate the effects of quercetin on oxidative stress and apoptosis in testicular tissue of streptozotocin-induced diabetic male rats. Methods: Thirty-two Wistar type Albino rats were divided into 4 groups. The groups are planned as follows: group 1: control group (only 0.9% sodium chloride, i.p. injection for 30 days, n = 8), group 2: DM group (streptozotocin: 50 mg/kg, freshly dissolved in 0.9% sodium chloride, i.p, n = 8), group 3: DM + QR group (quercetin: 20 mg/kg/day, diluted in 4% ethanol, 30 days, i.p.) (streptozotocin: 50 mg/kg, i.p., single dose, n = 8), group 4: control + QR group (quercetin: 20 mg/kg/day, 30 day, i.p., n = 8). Testicular tissue samples were taken under anesthesia on the 31st day of the study. Malondialdehyde, lipid hydroperoxide, advanced oxidation protein products, total thiol, nonprotein sulfhydryl compounds, and Cu,Zn-superoxide dismutase were determined by spectrophotometric method. Results: Malondialdehyde and lipid hydroperoxide levels were higher in the DM group compared to the control and DM+ QR group. Advanced oxidation protein product levels were lower in the control group than in the control+ QR and DM group, while total thiol, nonprotein sulfhydryl levels, and Cu,Zn-superoxide dismutase activity were higher in DM+ QR group compared to control and DM groups. In the DM+ QR group, a significant decrease was found in the number of apoptotic cells compared to the DM group (P < .001). Conclusion: Quercetin administration can be potentially beneficial and effective in preventing oxidative stress (lipid and protein) damage and apoptosis in the testicles of diabetic rats.

Özet

Amaç: Bu çalışmada, streptozotosin ile diyabet oluşturulan erkek sıçanlarda testis dokusunda kuersetin’in oksidatif stres ve apoptoz üzerindeki etkilerini araştırmak amaçlanmıştır. Yöntemler: Çalışma kapsamında 32 erkek Wistar Albino sıçan 4 gruba ayrıldı. Grup 1: Kontrol grubu (30 gün boyunca sadece intraperitoneal %0,9 sodyum klorür uygulandı, n = 8), grup 2: Diyabet grubu (DM grubu, %0,9 sodyum klorür çözeltisinde taze olarak hazırlanmış 50 mg/kg streptozotosinin intraperitoneal olarak enjeksiyonu ile oluşturuldu, n = 8), grup 3: Diyabet + Kuersetin grubu (DM+QR grubu, Tek doz 50 mg/kg streptozotosin intraperitoneal enjekte edildi ve 30 gün boyunca %4 etanol çözeltisinde seyreltilmiş 20 mg/kg/gün kuersetin intraperitoneal uygulandı, n = 8), grup 4: Kontrol + Kuersetin grubu (Kontrol +QR grubu, 30 gün boyunca 20 mg/kg/gün kuersetin intraperitoneal uygulandı, n = 8). Çalışmanın 31. gününde anestezi altında testis dokusu örnekleri alındı. Malondialdehit