EcoRI Polymorphism in Intron 6 of the Bovine Lactoferrin Gene in South Anatolian Red and East Anatolian Red Cattle Breeds

DAK ırkı sığırlarda A allelinin frekansının B allelinin frekansına oranla yüksek olduğu ve GAK ırkı sığırlarda ise B allelinin frekansının A allelinin frekansına oranla yüksek olduğu tespit edilmiştir.

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Amaç & Hipotez:ABSTRACTLactoferrin (LF) is an iron-binding glycoprotein that exists in variety of biological fluids such as milk, tear, saliva, bile, mucous secretions and blood.

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Temel Bulgular & Veri:DNA samples of 46 SAR and 46 EAR cattle were selected from DNA collection obtained from previous research projects.

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Sonuç & Katkı:DAK ırkı sığırlarda A allelinin frekansının B allelinin frekansına oranla yüksek olduğu ve GAK ırkı sığırlarda ise B allelinin frekansının A allelinin frekansına oranla yüksek olduğu tespit edilmiştir.

🔬Tür:Araştırma Makalesi

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Sığır Laktoferrin geni · Güney Anadolu Kırmızısı · Doğu Anadolu Kırmızısı

Özet

ABSTRACTLactoferrin (LF) is an iron-binding glycoprotein that exists in variety of biological fluids such as milk, tear, saliva, bile, mucous secretions and blood. Bovine mastitis is an inflammation of the mammary gland. LF has a vital role in the defense mechanism of the mammary gland and the bovine LF gene is a potential candidate gene for imparting resistance to mastitis in dairy cows. The objective of the study is to determine the genotype and allele frequencies for bovine lactoferrin gene polymorphisms in South Anatolian Red (SAR) and East Anatolian Red (EAR) cattle. DNA samples of 46 SAR and 46 EAR cattle were selected from DNA collection obtained from previous research projects. Bovine LF gene was amplified by polymerase chain reaction (PCR) and the PCR products were digested by EcoRI restriction enzyme. The frequency of A allele was found higher than B allele in EAR cattle breed and the frequency of B allele was found higher than A allele in SAR cattle breed. We suggest that further studies should be conducted on the bovine LF gene in Bos taurus and Bos indicus cattle to understand the reason of high genotype frequency in SAR and EAR cattle breeds.Key Words: Bovine Lactoferrin gene, South Anatolian Red cattle, East Anatolian Red cattle ÖZET DOĞU ANADOLU KIRMIZISI VE GÜNEY ANADOLU KIRMIZISI IRKI SIĞIRLARDA LAKTOFERRİN GENİNİN 6. İNTRONUNDAKİ ECORI POLİMORFİZMİ Laktoferrin (LF) süt, gözyaşı, tükürük, safra, mukus salgıları ve kan gibi çeşitli biyolojik sıvılarda bulunan, demir bağlayıcı özelliği olan bir glukoproteindir. LF meme bezi savunma mekanizmasında önemli bir role sahiptir ve sığır laktoferrin geni sütçü ineklerin mastite karşı direnç şekillenmesinde potansiyel aday bir gendir. Bu çalışmada Güney Anadolu Kırmızısı (GAK) ve Doğu Anadolu Kırmızısı (DAK) sığırlarında, laktoferrin genindeki polimorfizmlerin genotip ve allel dağılımlarının belirlenmesi amaçlanmıştır. Çalışmada kullanılan 46 DAK ve 46 GAK DNA örneği, önceki yapılan çalışmalar sonucu elde ettiğimiz DNA koleksiyonundan temin edilmiştir. Hedef bölge polimeraz zincir reaksiyonu (PZR) ile çoğaltılmış ve PZR ürünleri EcoRI restriksiyon enzimi ile kesilmiştir. DAK ırkı sığırlarda A allelinin frekansının B allelinin frekansına oranla yüksek olduğu ve GAK ırkı sığırlarda ise B allelinin frekansının A allelinin frekansına oranla yüksek olduğu tespit edilmiştir. GAK ve DAK sığır ırklarında tespit edilen yüksek genotip frekansının sebebinin daha iyi anlaşılabilmesi için Bos taurus and Bos indicus ırkı sığırlarında laktoferrin genine yönelik çalışmaların yapılmasını önermekteyiz.Anahtar Kelimeler: Sığır Laktoferrin geni, Güney Anadolu Kırmızısı, Doğu Anadolu Kırmızısı

Abstract

Lactoferrin (LF) is an iron-binding glycoprotein that exists in variety of biological fluids such as milk, tear, saliva, bile, mucous secretions and blood. Bovine mastitis is an inflammation of the mammary gland. LF has a vital role in the defense mechanism of the mammary gland and the bovine LF gene is a potential candidate gene for imparting resistance to mastitis in dairy cows. The objective of the study is to determine the genotype and allele frequencies for bovine lactoferrin gene polymorphisms in South Anatolian Red (SAR) and East Anatolian Red (EAR) cattle. DNA samples of 46 SAR and 46 EAR cattle were selected from DNA collection obtained from previous research projects. Bovine LF gene was amplified by polymerase chain reaction (PCR) and the PCR products were digested by EcoRI restriction enzyme. The frequency of A allele was found higher than B allele in EAR cattle breed and the frequency of B allele was found higher than A allele in SAR cattle breed. We suggest that further studies should be conducted on the bovine LF gene in Bos taurus and Bos indicus cattle to understand the reason of high genotype frequency in SAR and EAR cattle breeds.

Anahtar kelimeler

Sığır Laktoferrin geni Güney Anadolu Kırmızısı Doğu Anadolu Kırmızısı Bovine Lactoferrin gene South Anatolian Red cattle East Anatolian Red cattle

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Bu Makaleye Atıf Yap

GÜRSEL, F. E., AKIŞ, İ., ATEŞ, A., YARDİBİ, H., HOŞTÜRK, G. T., ÖZTABAK, K. (2013). EcoRI Polymorphism in Intron 6 of the Bovine Lactoferrin Gene in South Anatolian Red and East Anatolian Red Cattle Breeds. Acta Veterinaria Eurasia, Cilt: 39 Sayı: 2, 183–188. https://ajindex.com/articles/980922

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Polish Black and White Holstein AA: 0.63 AB: 0.31 BB: 0.04 Sender et al., 2010 Polish Black and White AA: 0.37 AB: 0.59 BB: 0.02 Wodjak-Maksymiec et al., 2006 BB genotype frequencies in Polish cattle breeds were found to be very low, ranging between 0.005 and 0.1 (Table 2), whereas higher frequencies in SAR and EAR cattle were found, 0.30 and 0.13 respectively. Sender et al. (2010) suggested that the low frequency of BB genotype might occur due to prior selection which might be associated with milk yield, led to B allele elimination. In this study, higher BB genotype frequencies found in two different Anatolian cattle breeds, which might be interpreted with the non-prior selection of SAR and EAR cattle breeds related with the milk yield. Near Eastern cattle breeds display an asymmetrical genome composition with very few Zebu mtDNA haplotypes, sparse Zebu Ychromosomal markers and significant autosomal Zebu levels. It has been suggested that original gene pool were taurine and then subjected to introgression from Zebu population over time (Edwards et al., 2007). Genotype and allele frequencies of autosomal genes like PRL, GHRH-R, IGF-1 and DGAT-1 genes in SAR and EAR cattle breeds were found closer to Zebu cattle than European cattle breeds (Akis et al., 2010; Eken, 2010; Kaupe et al., 2004; Öztabak et al., 2008). Presence of Zebuassociated alleles in SAR and EAR might be explained by Zebu introgression to the Anatolian cattle breeds (Edwards et al., 2007; Loftus et al., 1999). The relatively higher frequency of BB genotype may also be a result of Zebu introgression. Studies on polymorphisms of Zebu lactoferrin gene must be conducted to support this suggestion. As a result, AA homozygote and AB heterozygote genotype frequencies in SAR and EAR cattle breeds were found similar with the genotype frequencies in Polish Black and White Holstein, Polish Holstein and Polish Friesian cattle breeds. BB genotype frequency was found higher in SAR and EAR cattle breeds than the Polish cattle. We suggest that further studies should be conducted on the bovine LF gene in Bos taurus and Bos indicus cattle breeds to understand the reason of high genotype frequency in SAR and EAR cattle breeds. REFERENCES Akis, I., Oztabak, K., Gonulalp, I., Mengi A., Un, C., 20 IGF-1 and IGF-1R gene polymorphisms in East Anatolian Red and South Anatolian Red Cattle breeds. Russian Journal of Genetics 46 (4), 439-442.
Barkema, H.W., Schukken, Y.H., Lam, T.J.G.M., Beiboer, M.L., Wilmink, H., Be