KaydedilenlerDerginizi ekleyin

Dergiler / Journal of Scientific Reports-A / 2023 / Sayı: 054

INVESTIGATION OF RECENTLY ABUSED DRUGS IN DRIED BLOOD SPOTS USING FTA CARDS BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY: VALIDATION AND APPLICATION TO REAL SAMPLES

Yeter EROL ÖZTÜRK
Sayfa
251–263
Yayın Tarihi
2023-09-30
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Application to real DBS samples showed that this method is a good alternative and useful technique for the detection of drugs of abuse in forensic toxicology and appears to provide a good alternative storage condition.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:A robust and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established for the analysis of 19 illicit drugs and metabolites in whole blood dried blood spots (DBS) using FTA cards.
📊
Temel Bulgular & Veri:Analytical separation of the analytes was maintained using an Agilent Poroshell column with mobile phase A (0.1% formic acid in water) and B (methanol).
💡
Sonuç & Katkı:Application to real DBS samples showed that this method is a good alternative and useful technique for the detection of drugs of abuse in forensic toxicology and appears to provide a good alternative storage condition.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:DBS · LC-MS/MS · drug abuse

Abstract

A robust and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established for the analysis of 19 illicit drugs and metabolites in whole blood dried blood spots (DBS) using FTA cards. Samples prepared using Ahlstrom Munksjö Gensaver™ Colorcards were extracted with methanol. Analytical separation of the analytes was maintained using an Agilent Poroshell column with mobile phase A (0.1% formic acid in water) and B (methanol). Multiple Reaction Monitoring (MRM) with positive ionisation on LC-MS/MS was used to develop the method. The method was validated and met the acceptance criteria with acceptable results for carry-over, linearity, specificity, sensitivity, accuracy, precision, matrix effect and recovery. The method was applied to positively reported whole blood samples from patients suspected of drug abuse. Good quantitative agreement was obtained between the DBS and whole blood methods. Application to real DBS samples showed that this method is a good alternative and useful technique for the detection of drugs of abuse in forensic toxicology and appears to provide a good alternative storage condition.

🔖

Bu Makaleye Atıf Yap

ÖZTÜRK, Y. E. (2023). INVESTIGATION OF RECENTLY ABUSED DRUGS IN DRIED BLOOD SPOTS USING FTA CARDS BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY: VALIDATION AND APPLICATION TO REAL SAMPLES. Journal of Scientific Reports-A, Sayı: 054, 251–263. https://ajindex.com/articles/964529

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (13)

  1. Sadler Simões, S., Castañera Ajenjo, A., and Dias, M.J. (2018). Dried blood spots combined to an UPLC-MS/MS method for the simultaneous determination of drugs of abuse in forensic toxicology. J Pharm Biomed Anal, 5,147,634-644
  2. Grüner, N., Stambouli, O., and Ross, R.S. (2015). Dried blood spots preparing and processing for use in immunoassays and in molecular techniques. J Vis Exp, 13(97), 52619
  3. Quraishi, R., Jain, R., and Ambekar, A. (2013). The use of dried blood spot samples in screening drugs of abuse. Pharmacology & Pharmacy, 4(2), 152-159
  4. Alfazil, A.A., and Anderson, R.A. (2008). Stability of benzodiazepines and cocaine in blood spots stored on filter paper. J Anal Toxico, 32(7), 511-515
  5. Garcia Boy, R., Henseler, R., Mattern, R., and Skopp, G. (2008). Determination of morphine and 6-acetylmorphine in blood with use of dried blood spots. Ther Drug Monit, 30(6), 733-739
  6. Beaudette, P., and Bateman, K.P. (2004). Discovery stage pharmacokinetics using dried blood spots, J Chromatogr B Analyt Technol Biomed Life Sci. 809(1), 153-158
  7. Barfield, M., Spooner, N., Lad, R., Parry, S., and Fowles, S. (2008). Application of dried blood spots combined with HPLC-MS/MS for the quantification of acetaminophen in toxicokinetic studies. J Chromatogr B Analyt Technol Biomed Life Sci, 870(1), 32-37
  8. Spooner, N., Lad, R., and Barfield, M. (2009). Dried blood spots as a sample collection technique for the determination of pharmacokinetics in clinical studies: considerations for the validation of a quantitative bioanalytical method. Anal Chem, 81(4), 1557-1563
  9. Dainty, T.C., Richmond, E.S., Davies, I., and Blackwell, M.P. (2012). Dried blood spot bioanalysis: an evaluation of techniques and opportunities for reduction and refinement in mouse and juvenile rat toxicokinetic studies. Int J Toxicol, 31, 4
  10. Velghe, S., Troyer, R.D., and Stove, C. (2018). Dried blood spots in therapeutic drug monitoring and toxicology. Expert Opin Drug Metab Toxicol, 14, 1-3
  11. Edelbroek, P.M., Van der Heijden, J., and Stolk, L.M. (2009). Dried blood spot methods in therapeutic drug monitoring: methods, assays, and pitfalls. Ther Drug Monit, 31, 327
  12. McDade, T.W., Williams, S., and Snodgrass, J.J. (2007). What a drop can do: dried blood spots as a minimally invasive method for integrating biomarkers into population-based research. Demography, 44, 899
  13. Den Burger, J.C., Wilhelm, A.J., Chahbouni, A., Vos, R.M., Sinjewel, A., and Swart, E.L.