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Dergiler / Experimed / 2023 / Cilt: 13 Sayı: 2

Investigation of the Toxicologic and Biochemical Effects of Silk Fibroin/Gold Nanoparticles-Based Nanofiber Using Zebrafish Embryos

Ozan ÖZCAN
İsmail ÜNAL
Elif TUFAN
Ebru EMEKLİ ALTURFAN
Tuğba TUNALI-AKBAY
Sayfa
109–114
Yayın Tarihi
2023-09-18
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Conclusion: SF/AuNPs-based nanofiber did not affect the hatchability and mortality of embryos but increased oxidant damage, therefore it is thought that this oxidant effect of SF/AuNPs-based nanofibers may provide antibacterial properties.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objective: This study aimed to test the toxicity of silk fibroin (SF) / gold nanoparticles (AuNPs)-based nanofiber by using zebrafish embryos as an alternative animal model.
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Temel Bulgular & Veri:The zebrafish embryos were divided into four groups as control, 3,4-dichloroaniline (DCA) treated, one day SF/AuNPs treated (1D), and seven days SF/AuNPs treated (7D) group.
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Sonuç & Katkı:Conclusion: SF/AuNPs-based nanofiber did not affect the hatchability and mortality of embryos but increased oxidant damage, therefore it is thought that this oxidant effect of SF/AuNPs-based nanofibers may provide antibacterial properties.
🔬Tür:Modelleme & Sayısal Simülasyon
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Silk fibrion · gold nanoparticles · zebrafish embryos

Abstract

Objective: This study aimed to test the toxicity of silk fibroin (SF) / gold nanoparticles (AuNPs)-based nanofiber by using zebrafish embryos as an alternative animal model. Materials and Methods: Nanofiber was fabricated via electrospinning. The zebrafish embryos were divided into four groups as control, 3,4-dichloroaniline (DCA) treated, one day SF/AuNPs treated (1D), and seven days SF/AuNPs treated (7D) group. The SF/AuNPs nanofiber was incubated in the medium for one day and seven days. Following incubation, the embryos were placed in the mediums and their development was monitored 72 hours post-fertilization. In the zebrafish embryos, levels of malondialdehyde (MDA), nitric oxide (NO), activities of superoxide dismutase (SOD) and glutathione-S-transferase (GST) were detected. Results: Compared to the control group, there was no change in the hatching and mortality rates in the 1D and 7D groups. In the DCA group, the mortality rate was higher than the controls. In the 1D and 7D groups, MDA and NO were higher than the control but lower than the DCA group. SOD and GST activities decreased compared to the control. Conclusion: SF/AuNPs-based nanofiber did not affect the hatchability and mortality of embryos but increased oxidant damage, therefore it is thought that this oxidant effect of SF/AuNPs-based nanofibers may provide antibacterial properties.

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Bu Makaleye Atıf Yap

ÖZCAN, O., ÜNAL, İ., TUFAN, E., ALTURFAN, E. E., TUNALI-AKBAY, T. (2023). Investigation of the Toxicologic and Biochemical Effects of Silk Fibroin/Gold Nanoparticles-Based Nanofiber Using Zebrafish Embryos. Experimed, Cilt: 13 Sayı: 2, 109–114. https://ajindex.com/articles/962016

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