An Overview of Adding Rho-Associated Coiled-Coil Kinase and Knockout Serum Replacement with Trehalose to a Low Glycerol Tris-Based Semen Extender

It has been explained that low dose glycerol (trehalose added to increase the cryoprotectant effect) added to the extender in the freezing of livestock animal semen, knockout serum replacement (KSR) and Rho-associated coiled-coil kinase (ROCK), which are antioxidant additives, can increase the in vitro quality parameters of frozen thawed semen.

📌 3 Maddede Bu Makale

🎯

Amaç & Hipotez:It is known that livestock animal semen is very sensitive to cold shock during freezing processes, and this sensitivity directly affects post-thaw sperm qualities which are progressive motility, mitochondrial membrane potential, sperm nuclear DNA integrity and in vitro spermatological parameters such as plasma membrane and acrosome integrity, and sperm fertility.

📊

Temel Bulgular & Veri:In addition, with the sudden decrease in the total antioxidant level of the semen after thawing, the sperm cells are insufficient to tolerate their damage.

💡

Sonuç & Katkı:It has been explained that low dose glycerol (trehalose added to increase the cryoprotectant effect) added to the extender in the freezing of livestock animal semen, knockout serum replacement (KSR) and Rho-associated coiled-coil kinase (ROCK), which are antioxidant additives, can increase the in vitro quality parameters of frozen thawed semen.

🔬Tür:Araştırma Makalesi

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Sperm · Extender · Glycerol

Abstract

It is known that livestock animal semen is very sensitive to cold shock during freezing processes, and this sensitivity directly affects post-thaw sperm qualities which are progressive motility, mitochondrial membrane potential, sperm nuclear DNA integrity and in vitro spermatological parameters such as plasma membrane and acrosome integrity, and sperm fertility. In addition, with the sudden decrease in the total antioxidant level of the semen after thawing, the sperm cells are insufficient to tolerate their damage. Consequently, significant losses occur in sperm fertility. For this reason, researches on freezing the semen of livestock animals include semen processing; cryopreservation/cryogenic damage – thawing methods - sperm extenders, added antioxidants, the mechanisms of action and metabolic pathways of these antioxidants and physiological and metabolic parameters such as sperm fertility. It has been explained that low dose glycerol (trehalose added to increase the cryoprotectant effect) added to the extender in the freezing of livestock animal semen, knockout serum replacement (KSR) and Rho-associated coiled-coil kinase (ROCK), which are antioxidant additives, can increase the in vitro quality parameters of frozen thawed semen.

Anahtar kelimeler

Sperm Extender Glycerol Trehalose Antioxidant KSR ROCK

🔖

Bu Makaleye Atıf Yap

ARSLAN, H. O., KELES, E., ROSTAMİ, B., ARSLAN-ACAROZ, D., SOLEİMANZADEH, A., BENNOUNE, O., AYAD, A., RAHLA, M., AKINCI, I. (2023). An Overview of Adding Rho-Associated Coiled-Coil Kinase and Knockout Serum Replacement with Trehalose to a Low Glycerol Tris-Based Semen Extender. Black Sea Journal of Agriculture, Cilt: 6 - Sayı: 2, 210–214. https://ajindex.com/articles/885328

📄 Tam Metin PDF Okuyucu

Yeni Sekmede Aç ↗

↗Yeni Sekme

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (14)

Aboagla EME, Terada T. 2003. Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biol Reprod, 69: 1245-1250.
Aisen EG, Alvarez HI, Venturino A, Gadre JJ. 2000. Effect of trehalose and EDTA on cryoprotective action of ram semen diluents. Theriogenology, 53: 1053-1061.
Aisen EG, Medina VH, Venturino A. 2002. Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology, 57: 1801-1808.
Aisen EG, Quintana M, Medina V, Morello H, Venturino A. 2005. Ultramicroscopic and biochemical changes in ram spermatozoa cryopreserved with trehalose-based hypertonic extenders. Cryobiol, 50: 239-249.
Aitken RJ, Baker MA. 2004. Oxidative stress and male reproductive biology. Reprod Fertil Dev, 16: 581-588.
Alvarenga MA, Papa FO, Landim-Alvarenga FC, Medeiros A.SL. 2005. Amides as cryoprotectants for freezing stallion semen. Anim Reprod Sci, 89: 105-113.
Alvarez JG, Storey BT. 1983. Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility. Biol Reprod, 29: 548-555.
Alvarez JG, Storey BT. 1984. Assessment of cell damage caused by spontaneous lipid peroxidation in rabbit spermatozoa. Biol Reprod, 30: 833-841.
Amini S, Masoumi R, Rostami B, Shahir MH, Taghilou P, Arslan HO. 2019. Effects of supplementation of Tris-egg yolk extender with royal jelly on chilled and frozen-thawed ram semen characteristics. Cryobiol, 88: 75-80.
Aoshima K, Baba A, Makino Y, Okada Y. 2013. Establishment of alternative culture method for spermatogonial stem cells using knockout serum replacement. PLoS One, 8(10): e77715. Arav A, Hehu D, Mattioli M. 1993. Osmotic and cytotoxic study of vitrification of ımmature bovine oocytes. J Reprod Fertil, 99: 353-358.
Ark M, Özdemir A, Polat B. 2010. Ouabain-induced apoptosis and Rho kinase: a novel caspase-2 cleavage site and fragment of Rock-2. Apoptosis, 15(12): 1494-1506.
Arslan HO, Herrera C, Malama E, Siuda M, Leiding C, Bollwein,H. 2019. Effect of the addition of different catalase concentrations to a TRIS-egg yolk extender on quality and in vitro fertilization rate of frozen-thawed bull sperm. Cryobiol, 91: 40-52.
Bilodeau JF, Blanchette S, Gagnon IC, Sirard MA. 2001. Thiols prevent H2O2-mediated loss of spermmotility in cryopreserved bullsemen. Theriogenology, 56: 275-286.
Bilodeau JF, Chatterjee S, Sirard MA, Gagnon C. 2000. Levels of antioxidant defenses are decreased in bovine spermatozoa