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Dergiler / Black Sea Journal of Agriculture / 2026 / Cilt: 9 Sayı: 5

Assessment of the endochitinase (ech42) Protein-Coding Gene as a DNA Barcode for Species Delimitation and Phylogenetic Reconstruction in Trichoderma

Yunus Korkom(ADNAN MENDERES UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF PLANT PROTECTION)0000-0001-5859-9026
Sayfa
895–901
Yayın Tarihi
2026-09-15
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

These findings demonstrate that the ech42 gene possesses significant potential as a complementary DNA barcode for Trichoderma species identification and phylogenetic studies.

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Amaç & Hipotez:Accurate species identification is a prerequisite for deciphering the ecological adaptation, evolutionary diversification, and functional potential of Trichoderma species.
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Temel Bulgular & Veri:In this study, the protein-coding gene of endochitinase 42 kDa (ech42) was evaluated as a DNA barcode for species delimitation and phylogenetic reconstruction in Trichoderma genus.
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Sonuç & Katkı:These findings demonstrate that the ech42 gene possesses significant potential as a complementary DNA barcode for Trichoderma species identification and phylogenetic studies.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Trichoderma guizhouense · Trichoderma harzianum · Amino acid

Özet

Accurate species identification is a prerequisite for deciphering the ecological adaptation, evolutionary diversification, and functional potential of Trichoderma species. It is providing a foundation for understanding their roles in host interactions, environmental fitness, and biocontrol applications. In this study, the protein-coding gene of endochitinase 42 kDa (ech42) was evaluated as a DNA barcode for species delimitation and phylogenetic reconstruction in Trichoderma genus. Phylogenetic inferences were drawn from eight local isolates representing four Trichoderma species. Sequence alignment of the ech42 gene generated a dataset of approximately 850 bp, providing sufficient phylogenetically informative characters for species-level discrimination. Phylogenetic analyses revealed that the ech42 marker consistently resolved all four species, with bootstrap support of 100%. Physicochemical and three-dimensional structural analyses of ech42 gene revealed that their amino acid numbers ranged from 278 to 283 residues in Trichoderma species and T. virens exhibited the most hydrophilic protein, with a GRAVY value of −0.303. These findings demonstrate that the ech42 gene possesses significant potential as a complementary DNA barcode for Trichoderma species identification and phylogenetic studies.

Abstract

Accurate species identification is a prerequisite for deciphering the ecological adaptation, evolutionary diversification, and functional potential of Trichoderma species. It is providing a foundation for understanding their roles in host interactions, environmental fitness, and biocontrol applications. In this study, the protein-coding gene of endochitinase 42 kDa (ech42) was evaluated as a DNA barcode for species delimitation and phylogenetic reconstruction in Trichoderma genus. Phylogenetic inferences were drawn from eight local isolates representing four Trichoderma species. Sequence alignment of the ech42 gene generated a dataset of approximately 850 bp, providing sufficient phylogenetically informative characters for species-level discrimination. Phylogenetic analyses revealed that the ech42 marker consistently resolved all four species, with bootstrap support of 100%. Physicochemical and three-dimensional structural analyses of ech42 gene revealed that their amino acid numbers ranged from 278 to 283 residues in Trichoderma species and T. virens exhibited the most hydrophilic protein, with a GRAVY value of −0.303. These findings demonstrate that the ech42 gene possesses significant potential as a complementary DNA barcode for Trichoderma species identification and phylogenetic studies.

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Bu Makaleye Atıf Yap

Korkom, Y. (2026). Assessment of the endochitinase (ech42) Protein-Coding Gene as a DNA Barcode for Species Delimitation and Phylogenetic Reconstruction in Trichoderma. Black Sea Journal of Agriculture, Cilt: 9 Sayı: 5, 895–901. https://doi.org/10.47115/bsagriculture.2007898

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