Dergiler / Turkish Journal of Biology / 2000 / Cilt: 24 - Sayı: supp

Aspergillus niger' den ürikaz enziminin üretilmesi ve aktiviteye etkili bazı faktörlerin belirlenmesi

Production of uricase enzyme from Aspergillus niger and determinatin of some factors affecting the activity

Sayfa
11–23
Yayın Tarihi
2022-11-05
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

It was determined that ammonium compounds, which were added to the production medium, inhibited enzyme synthesis, and that MgS04 has an important role in enzyme synthesis.These results revealed that A. niger may be a benefical source of uricase enzyme.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:This study was undertaken to obtain uricase enzyme from Aspergillus niger and also to determine the production conditions and the effects of some factors on enzyme activity.
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Temel Bulgular & Veri:The results of the experiments, in consideration of the production of the uricase enzyme and the factors affecting the production, demonstrated that enzyme activity was maximum when the production time was 3 days at a temperature of 30ºC with the initial pH at 6.0 and inductor concentration at 0.1%.
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Sonuç & Katkı:It was determined that ammonium compounds, which were added to the production medium, inhibited enzyme synthesis, and that MgS04 has an important role in enzyme synthesis.These results revealed that A. niger may be a benefical source of uricase enzyme.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

This study was undertaken to obtain uricase enzyme from Aspergillus niger and also to determine the production conditions and the effects of some factors on enzyme activity. The results of the experiments, in consideration of the production of the uricase enzyme and the factors affecting the production, demonstrated that enzyme activity was maximum when the production time was 3 days at a temperature of 30ºC with the initial pH at 6.0 and inductor concentration at 0.1%. It was determined that ammonium compounds, which were added to the production medium, inhibited enzyme synthesis, and that MgS04 has an important role in enzyme synthesis.These results revealed that A. niger may be a benefical source of uricase enzyme.

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Bu Makaleye Atıf Yap

ERTAN, F., AKSÖZ, E. (2022). Aspergillus niger' den ürikaz enziminin üretilmesi ve aktiviteye etkili bazı faktörlerin belirlenmesi. Turkish Journal of Biology, Cilt: 24 - Sayı: supp, 11–23. https://ajindex.com/articles/654449

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  1. Fujiwara,S.,Ohashi,H., Noguchi, T., Comparison of intraperoxisomal localization from and properties of amphibianRana catasbeianauricase with those of other animal uricases. Comp. Biochem. Physiol. 86B, 1, 23 - 26. 1987. 2.Murray, R.K., Mayes, P.A., Granner, D.K., Rodwell, V.W., Harper’in Biyokimyas›, (Çev: G.Mentefl, B. Ersöz), ‹stan-bul, 1993, Bar›fl Kitabevi, 422 - 439. 3.Liu, J., Li, G., Liu, H., Zhou, X., Purification and properties of uricase Candida sp.and its application of uric acidanalysis in serum, Appl. Biochem. Biotechnol., 47, 1, 57 - 63, 1994. 4.Yeldandi, A.V., Wang, X., Alvares, K., Kumar, S., Rao, M.S., Reddy, J.K., Human urate oxidase gene: Cloningand partial sequence analysis reveal a stop codon within the fifth exon., Biochem, Biophys.Res.Comm., 171,2, 641 - 646, 1990. 5.Bhargava, A. K., Lal, H., Pundir, C.S., Discrete analysis of serum uric acid with immobilized uricase and peroxi-dase, J. Biochem., Biophys., Methods, 39,3,125-136,1999. 6.Mahler, J.L., A new bacterial uricase for uric acid determination, Analyt.Biochem., 38, 65 - 84, 1970. 7.Kinsella, J.E., German, B., Shetty, J., Uricase from fish liver: Isolation and some properties. Comp. Biochem.Physiol., 82 B, 621 - 624, 1985. 8.Montalbini, P., Redondo, J., Caballero, J., Pineda, M., Uricase from leaves: Its purification and characterizationfrom three different higher plants, Planta, 202, 3, 277-283, 1997. 9.Pawlotsky, Y., Hyperuricemia and gout: therapeutic indications. Rev. Prat., 44, 2, 206 - 209,1994. 10. Tsujino, Y., Yokoo, Y., Skato, K., Hair coloring and waving using oxidase, J. Soc. Cosm. Chem., 42, 4, 273 -282, 1992. 11. Nakagawa, S., Oda, H., Anazawa, H., High cell - density cultivation and high recombinant protein production ofEscherchia colistrain expressing uricase, Bio. Biotech. Biochem., 59, 12, 2263 -2267,1995. 12. Kan, J.Q., Zhou, F., Mu, S.L., Shi, Y.J., Effect of alpha, alpha’- bipyridine on activity of the polyanilineuricase electrode, Sens., Act., B- Chem., 30,1, 7-10,1996. 13. Miland. E., Ord›eres, A.J.M., Blanco, P.T., Smyth, M.R., Fagain, C.O., Poly (o - aminophenol) - modified bien-zyme carbon - pastle electrode for the detection of uric acid, Talanta, 43, 5, 785 - 796,1996. 14. Hasebe, Y., Nawa, K., Ujita, S., Uchiama, S., High sensitive flow detection of uric acid based on an intermediateregeneration of uricase, Analyst., 123, 8, 1775-1780, 1998. 15. Nakaminami, T., Ito, S., Kuwabata, S., Yoneyama, H., Uricase-catalysed oxidation of uric acid artificial