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Journals / Turkish Journal of Biology / 2000 / Cilt: 24 - Sayı: supp

Production of uricase enzyme from Aspergillus niger and determinatin of some factors affecting the activity

Aspergillus niger' den ürikaz enziminin üretilmesi ve aktiviteye etkili bazı faktörlerin belirlenmesi

Figen ERTAN
Erol AKSÖZ
Pages
11–23
Publication Date
2022-11-05
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

It was determined that ammonium compounds, which were added to the production medium, inhibited enzyme synthesis, and that MgS04 has an important role in enzyme synthesis.These results revealed that A. niger may be a benefical source of uricase enzyme.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study was undertaken to obtain uricase enzyme from Aspergillus niger and also to determine the production conditions and the effects of some factors on enzyme activity.
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Key Findings & Data:The results of the experiments, in consideration of the production of the uricase enzyme and the factors affecting the production, demonstrated that enzyme activity was maximum when the production time was 3 days at a temperature of 30ºC with the initial pH at 6.0 and inductor concentration at 0.1%.
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Conclusion & Impact:It was determined that ammonium compounds, which were added to the production medium, inhibited enzyme synthesis, and that MgS04 has an important role in enzyme synthesis.These results revealed that A. niger may be a benefical source of uricase enzyme.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

This study was undertaken to obtain uricase enzyme from Aspergillus niger and also to determine the production conditions and the effects of some factors on enzyme activity. The results of the experiments, in consideration of the production of the uricase enzyme and the factors affecting the production, demonstrated that enzyme activity was maximum when the production time was 3 days at a temperature of 30ºC with the initial pH at 6.0 and inductor concentration at 0.1%. It was determined that ammonium compounds, which were added to the production medium, inhibited enzyme synthesis, and that MgS04 has an important role in enzyme synthesis.These results revealed that A. niger may be a benefical source of uricase enzyme.

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Cite This Article

ERTAN, F., AKSÖZ, E. (2022). Production of uricase enzyme from Aspergillus niger and determinatin of some factors affecting the activity. Turkish Journal of Biology, Cilt: 24 - Sayı: supp, 11–23. https://ajindex.com/articles/654449

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References (1)

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