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Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2000 / Cilt: 24 - Sayı: 5

Escherichia coli 0157:H7 serotipinin izolasyonunda selektif broth ve agar besiyerleri üzerine bir çalışma

A study on selective broths and agar media for the isolation of Escherichia coli 0157:H7 serotype

Malihe R. NOVEIR
A. Kadir HALKMAN
Sayfa
459–464
Yayın Tarihi
2022-11-05
DOI
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This is probably more important than the minimum recovery rate of culture media and/or conventional culture methods when E. coli O157:H7 exists without competitive microorganisms.

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Amaç & Hipotez:Modified tryptic soy broth, lauryl sulfate tryptose broth, modified EC broth, sorbitol MacConkey agar and Fluorocult E. coli O157 agar were tested for the recovery of 3 strains of Escherichia coliO157:H7 against E. colitype 1 and Citrobacter freundii as common competitive microflora of raw meat products.
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Temel Bulgular & Veri:Two-stepped trials showed that modified EC broth and lauryl sulfate tryptose broth were superior to modified tryptic soy broth as selective enrichment media while sorbitol MacConkey agar was clearly better than Fluorocult E. coli O157 agar as a selective agar medium.
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Sonuç & Katkı:This is probably more important than the minimum recovery rate of culture media and/or conventional culture methods when E. coli O157:H7 exists without competitive microorganisms.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

Modified tryptic soy broth, lauryl sulfate tryptose broth, modified EC broth, sorbitol MacConkey agar and Fluorocult E. coli O157 agar were tested for the recovery of 3 strains of Escherichia coliO157:H7 against E. colitype 1 and Citrobacter freundii as common competitive microflora of raw meat products. Two-stepped trials showed that modified EC broth and lauryl sulfate tryptose broth were superior to modified tryptic soy broth as selective enrichment media while sorbitol MacConkey agar was clearly better than Fluorocult E. coli O157 agar as a selective agar medium. Recovery of E. coli O157:H7 was difficult when selective enrichment broth-growing cohabitant microflora were present at more than 100 folds of E. coliO157. One percent E. coli O157:H7 in the cohabitant flora was the minimum level for recovery. This is probably more important than the minimum recovery rate of culture media and/or conventional culture methods when E. coli O157:H7 exists without competitive microorganisms.

Abstract

Modified tryptic soy broth, lauryl sulfate tryptose broth, modified EC broth, sorbitol MacConkey agar and Fluorocult E. coli O157 agar were tested for the recovery of 3 strains of Escherichia coliO157:H7 against E. colitype 1 and Citrobacter freundii as common competitive microflora of raw meat products. Two-stepped trials showed that modified EC broth and lauryl sulfate tryptose broth were superior to modified tryptic soy broth as selective enrichment media while sorbitol MacConkey agar was clearly better than Fluorocult E. coli O157 agar as a selective agar medium. Recovery of E. coli O157:H7 was difficult when selective enrichment broth-growing cohabitant microflora were present at more than 100 folds of E. coliO157. One percent E. coli O157:H7 in the cohabitant flora was the minimum level for recovery. This is probably more important than the minimum recovery rate of culture media and/or conventional culture methods when E. coli O157:H7 exists without competitive microorganisms.

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Bu Makaleye Atıf Yap

NOVEIR, M. R., HALKMAN, A. K. (2022). Escherichia coli 0157:H7 serotipinin izolasyonunda selektif broth ve agar besiyerleri üzerine bir çalışma. Turkish Journal of Veterinary and Animal Sciences, Cilt: 24 - Sayı: 5, 459–464. https://ajindex.com/articles/615735

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Kaynakça (14)

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