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Journals / Turkish Journal of Veterinary and Animal Sciences / 2000 / Cilt: 24 - Sayı: 5

A study on selective broths and agar media for the isolation of Escherichia coli 0157:H7 serotype

Escherichia coli 0157:H7 serotipinin izolasyonunda selektif broth ve agar besiyerleri üzerine bir çalışma

Malihe R. NOVEIR
A. Kadir HALKMAN
Pages
459–464
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This is probably more important than the minimum recovery rate of culture media and/or conventional culture methods when E. coli O157:H7 exists without competitive microorganisms.

📌 3 Key Takeaways

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Objective & Hypothesis:Modified tryptic soy broth, lauryl sulfate tryptose broth, modified EC broth, sorbitol MacConkey agar and Fluorocult E. coli O157 agar were tested for the recovery of 3 strains of Escherichia coliO157:H7 against E. colitype 1 and Citrobacter freundii as common competitive microflora of raw meat products.
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Key Findings & Data:Two-stepped trials showed that modified EC broth and lauryl sulfate tryptose broth were superior to modified tryptic soy broth as selective enrichment media while sorbitol MacConkey agar was clearly better than Fluorocult E. coli O157 agar as a selective agar medium.
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Conclusion & Impact:This is probably more important than the minimum recovery rate of culture media and/or conventional culture methods when E. coli O157:H7 exists without competitive microorganisms.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Modified tryptic soy broth, lauryl sulfate tryptose broth, modified EC broth, sorbitol MacConkey agar and Fluorocult E. coli O157 agar were tested for the recovery of 3 strains of Escherichia coliO157:H7 against E. colitype 1 and Citrobacter freundii as common competitive microflora of raw meat products. Two-stepped trials showed that modified EC broth and lauryl sulfate tryptose broth were superior to modified tryptic soy broth as selective enrichment media while sorbitol MacConkey agar was clearly better than Fluorocult E. coli O157 agar as a selective agar medium. Recovery of E. coli O157:H7 was difficult when selective enrichment broth-growing cohabitant microflora were present at more than 100 folds of E. coliO157. One percent E. coli O157:H7 in the cohabitant flora was the minimum level for recovery. This is probably more important than the minimum recovery rate of culture media and/or conventional culture methods when E. coli O157:H7 exists without competitive microorganisms.

Özet

Modified tryptic soy broth, lauryl sulfate tryptose broth, modified EC broth, sorbitol MacConkey agar and Fluorocult E. coli O157 agar were tested for the recovery of 3 strains of Escherichia coliO157:H7 against E. colitype 1 and Citrobacter freundii as common competitive microflora of raw meat products. Two-stepped trials showed that modified EC broth and lauryl sulfate tryptose broth were superior to modified tryptic soy broth as selective enrichment media while sorbitol MacConkey agar was clearly better than Fluorocult E. coli O157 agar as a selective agar medium. Recovery of E. coli O157:H7 was difficult when selective enrichment broth-growing cohabitant microflora were present at more than 100 folds of E. coliO157. One percent E. coli O157:H7 in the cohabitant flora was the minimum level for recovery. This is probably more important than the minimum recovery rate of culture media and/or conventional culture methods when E. coli O157:H7 exists without competitive microorganisms.

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Cite This Article

NOVEIR, M. R., HALKMAN, A. K. (2022). A study on selective broths and agar media for the isolation of Escherichia coli 0157:H7 serotype. Turkish Journal of Veterinary and Animal Sciences, Cilt: 24 - Sayı: 5, 459–464. https://ajindex.com/articles/615735

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