Sjögren's syndrome: A study of salivary electrophoresis

Amaç & Hipotez:Many diagnostic tests exist to assess the salivary and lacrimal involvement in Sjögren's syndrome (SS), but there is still disagreement.

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Temel Bulgular & Veri:Saliva were collected from patients suffering from primary Sjögren's syndrome (n = 10) and a control group (n = 10).

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Sonuç & Katkı:Salivary electrophoresis can provide a simple, noninvasive, inexpensive, and comfortable method for the diagnosis of Sjögren's syndrome.

🔬Tür:Araştırma Makalesi

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Alan Parametreleri

Abstract

Many diagnostic tests exist to assess the salivary and lacrimal involvement in Sjögren's syndrome (SS), but there is still disagreement. The purpose of this study was to examine salivary proteins in patients with primary Sjögren's syndrome and healthy control subjects by the polyacrylamide gel electrophoresis, as well as the applicability of the method. The polyacrylamide gel electrophoresis, applied in our examinations, is a simple and rapid method, requiring only a minute amount of saliva for the analysis. Saliva were collected from patients suffering from primary Sjögren's syndrome (n = 10) and a control group (n = 10). All saliva samples were stored at -70 °C until use. The diagnosis of Sjögren's syndrome was verified by a biopsy of the minor glands of the lower lip in the patient group. Most of the patients with Sjögren's syndrome exhibited an electrophoretic profile that was different from healthy control subjects. Electrophoresis systems are most reliable for Sjögren’s syndrome. Our study suggests that the salivary electrophoresis are most reliable for Sjögren's syndrome. Salivary electrophoresis can provide a simple, noninvasive, inexpensive, and comfortable method for the diagnosis of Sjögren's syndrome.

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Bu Makaleye Atıf Yap

ERGEN, K., AYDIL, B., YALTIRIK, M., BERBEROĞLU, K. H. (2022). Sjögren's syndrome: A study of salivary electrophoresis. Turkish Journal of Medical Sciences, Cilt: 35 - Sayı: 6, 401–405. https://ajindex.com/articles/611952

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Kaynakça (12)

Rhodus LN. Sjögren's Syndrome Oral Medicine. Quintessence Int 30: 689-99, 1999
Al - Hashimi I, Haghıghat N, Fox PC. Salivary Electrophoresis in the Diagnosis of Sjögren Syndrome. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85: 542-547, 1998
Beeley A J. Clinical.Applications of Electrophoresis of Human Salivary Proteins. J Chromat, 569: 261-280, 1991
Moody M, Zipp M, Al-Hashimi 1. Salivary Anti-Spectrin. Autoantibodies in Sjögren's Syndrome. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 91: 322-327, 2001
Scully C. Sjögren's Syndrome: Clinical and laboratory features, immunopathogenesis, and management. Oral Surg Oral Med Oral Pathol, 62: 510-523, 1986
Fischer CJ, Wyshak GH, Weisberger D. Sjögren's syndrome electrophoretic and immunological observations on serum and salivary proteins of man. Archs oral Biol, 13: 257-270, 1968
Vitali C, Moutsopoulos HM, Bombardieri S. The European Community Study Group on Diagnostic Criteria for Sjögren's Syndrome. Sensitivity and Specificity of Tests For Ocular and Oral Involvement in Sjögren's Syndrome. Ann Rheum Dis, 53: 637-647, 1994
Spât BE, Puskas M, Rajki J. Electrophoretic Protein Pattern of Human Parotid Saliva in Sjögren's Syndrome and Sialosis. Acta Otolaryngol, 90: 275-282, 1980
Smith PK, Krohn RI, Hermanson GT et al. Measurement of Protein Using Bicinchoninic Acid. Anal Biochem 150: 76-85, 1985
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-85, 1970
Moody M, Zipp MM, Yasbin L et al. The Effect of Parotid salivary Flow Rate on the Levels of Salivary Antimicrobial Proteins in Patients with Sjögren's Syndrome. Oral Med Clin Res, 30: 700-705, 1999
Beeley AJ, Khoo SK, Lamey JP. Two Dimensional Electrophoresis of Human Parotid Salivary Proteins From Normal and Connective Tissue Disorder Subjects Using Immobilized pH Gradients. Electrophoresis 12: 493-499, 1991.