After 45 days of follow-up, therats were sacrificed, and the harvested tissues were histopathologically evaluated for EF.Results: Compared with Group A, Group B showed significantly less EF generation (p < 0.001), implyingthat dHACM allografts effectively prevent EF.Conclusions: This study demonstrated that dHACM can effectively reduce EF formation after spinallaminectomy in rats.
📌 3 Maddede Bu Makale
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Amaç & Hipotez:Objectives: Post-laminectomy syndrome due to epidural fibrosis (EF) is a common cause of persistent lowback pain after lumbar spinal surgery and is challenging for both surgeons and patients.
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Temel Bulgular & Veri:The current experimental study aimed to investigate the effect of dehydrated human amnion/chorion membrane (dHACM)allograft for preventing EF formation following lumbar laminectomy.Methods: Sixteen Sprague–Dawley rats equally divided into two groups underwent lumbar laminectomy.Group A, the control group, underwent lumbar laminectomy with standard closure.
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Sonuç & Katkı:After 45 days of follow-up, therats were sacrificed, and the harvested tissues were histopathologically evaluated for EF.Results: Compared with Group A, Group B showed significantly less EF generation (p < 0.001), implyingthat dHACM allografts effectively prevent EF.Conclusions: This study demonstrated that dHACM can effectively reduce EF formation after spinallaminectomy in rats.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri
Abstract
Objectives: Post-laminectomy syndrome due to epidural fibrosis (EF) is a common cause of persistent lowback pain after lumbar spinal surgery and is challenging for both surgeons and patients. The current experimental study aimed to investigate the effect of dehydrated human amnion/chorion membrane (dHACM)allograft for preventing EF formation following lumbar laminectomy.Methods: Sixteen Sprague–Dawley rats equally divided into two groups underwent lumbar laminectomy.Group A, the control group, underwent lumbar laminectomy with standard closure. Group B, the experimentalgroup, additionally received epidural dHACM allografts during the procedure. After 45 days of follow-up, therats were sacrificed, and the harvested tissues were histopathologically evaluated for EF.Results: Compared with Group A, Group B showed significantly less EF generation (p < 0.001), implyingthat dHACM allografts effectively prevent EF.Conclusions: This study demonstrated that dHACM can effectively reduce EF formation after spinallaminectomy in rats.
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Bu Makaleye Atıf Yap
Dalgıç, A., Menekşe, G., Belen, A. D., Uçkun, Ö. M., Divanlıoğlu, D., Akdağ, R. (2022). Dehydrated human amnion/chorion membrane allograft for preventing epidural fibrosis after laminectomy: an experimental rat model. The European Research Journal, Cilt: 6 - Sayı: 6, 534–538. https://ajindex.com/articles/597514
Burton CV, Kirkaldy-Willis WH, Yong-Hing K, Heithoff KB. Causes of failure of surgery on the lumbar spine. Clin Orthop Relat Res 1981:191-9
Fager CA, Freidberg SR. Analysis of failures and poor results of lumbar spine surgery. Spine (Phila Pa 1976) 1980;5:87-94
North RB, Campbell JN, James CS, Conover-Walker MK, Wang H, Piantadosi S, et al. Failed back surgery syndrome: 5- year follow-up in 102 patients undergoing repeated operation. Neurosurgery 1991;28:685-90; discussion 690-81
Ross JS, Robertson JT, Frederickson RC, Petrie JL, Obuchowski N, Modic MT, et al. Association between peridural scar and recurrent radicular pain after lumbar discectomy: magnetic resonance evaluation. ADCON-L European Study Group. Neurosurgery 1996;38:855-61; discussion 861-53
Tao H, Fan H. Implantation of amniotic membrane to reduce postlaminectomy epidural adhesions. Eur Spine J 2009;18:1202- 12
Einhaus SL, Robertson JT, Dohan FC, Jr, Wujek JR, Ahmad S. Reduction of peridural fibrosis after lumbar laminotomy and discectomy in dogs by a resorbable gel (ADCON-L). Spine 1997;22:1440-6; discussion 1446-7
Gill GG, Scheck M, Kelley ET, Rodrigo JJ. Pedicle fat grafts for the prevention of scar in low-back surgery. Spine 1985;10:662-7
Robertson JT, Meric AL, Dohan FC, Jr, Schweitzer JB, Wujek JR, Ahmad S. The reduction of postlaminectomy peridural fibrosis in rabbits by a carbohydrate polymer. J Neurosurg 1993;79:89-95
Wujek JR, Ahmad S, Harel A, Maier KH, Roufa D, Silver J. A carbohydrate polymer that effectively prevents epidural fibrosis at laminectomy sites in the rat. Exp Neurol 1991;114:237-45
Laurent GJ, Chambers RC, Hill MR, McAnulty RJ. Regulation of matrix turnover: fibroblasts, forces, factors and fibrosis. Biochem Soc Trans 2007;35:647-51
Boudreau N, Werb Z, Bissell MJ. Suppression of apoptosis by basement membrane requires three-dimensional tissue organization and withdrawal from the cell cycle. Proc Natl Acad Sci U S A 1996;93:3509-13
Dua HS, Azuara-Blanco A. Amniotic membrane transplantation. Br J Ophthalmol 1999;83:748-52. 13. von Versen-Höynck F, Syring C, Bachmann S, Möller DE. The influence of different preservation and sterilisation steps on the histological properties of amnion allografts--light and scanning electron microscopic studies. Cell Tissue Bank 2004;5:45-56
Koob TJ, Rennert R, Zabek N, Massee M, Lim JJ, Temenoff JS, et al. Biological properties of dehydrated human amnion/chorion composite graft: impli