Pharmacologically induced absence seizures versus kindling in Wistar rats

The mean numbers of stimulations needed for the development of the first stage 3, 4, or 5 generalized seizureswere significantly higher in the GBL+KI group than the KI group.CONCLUSION: The resistance to amygdala kindling in the GBL model can be modulated by the absence seizure mechanismalone, without the intervention of an abnormal genetic background.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:OBJECTIVE: This study aimed to investigate the effects of γ-butyrolactone (GBL), a prodrug of gamma-Hydroxybutyric acid-induced absence seizures on the development of kindling in Wistar rats.METHODS: Three groups of adult male Wistar rats under anesthesia were implanted with bilateral cortical recording electrodes for the GBL group (GBL) and/or bipolar stimulation electrodes into the right basolateral amygdala for the Kindlinggroup (KI) alone and Kindling plus GBL group (GBL+KI).

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Temel Bulgular & Veri:Rats in the KI and GBL+KI groups were stimulated twice daily atthe afterdischarge threshold until they reached Racine’s stage 5 seizure state.

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Sonuç & Katkı:The mean numbers of stimulations needed for the development of the first stage 3, 4, or 5 generalized seizureswere significantly higher in the GBL+KI group than the KI group.CONCLUSION: The resistance to amygdala kindling in the GBL model can be modulated by the absence seizure mechanismalone, without the intervention of an abnormal genetic background.

🔬Tür:Modelleme & Sayısal Simülasyon

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Alan Parametreleri

Abstract

OBJECTIVE: This study aimed to investigate the effects of γ-butyrolactone (GBL), a prodrug of gamma-Hydroxybutyric acid-induced absence seizures on the development of kindling in Wistar rats.METHODS: Three groups of adult male Wistar rats under anesthesia were implanted with bilateral cortical recording electrodes for the GBL group (GBL) and/or bipolar stimulation electrodes into the right basolateral amygdala for the Kindlinggroup (KI) alone and Kindling plus GBL group (GBL+KI). Rats in the KI and GBL+KI groups were stimulated twice daily atthe afterdischarge threshold until they reached Racine’s stage 5 seizure state. The animals in the GBL + group had an i.pinjection of GBL 20 minutes before each electrical stimulation, and the effects of GBL-induced seizures on the developmentof kindling were investigated. The animals in the GBL group were injected GBL twice daily i.p. for 15 days without receivingany electrical stimulation.RESULTS: The KI animals reached stage 5 seizure stage at 12th stimulations, whereas the GBL+KI rats reached at 27thstimulations. The mean numbers of stimulations needed for the development of the first stage 3, 4, or 5 generalized seizureswere significantly higher in the GBL+KI group than the KI group.CONCLUSION: The resistance to amygdala kindling in the GBL model can be modulated by the absence seizure mechanismalone, without the intervention of an abnormal genetic background.

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Bu Makaleye Atıf Yap

ONAT, F., EŞKAZAN, E., ÇARÇAK, N., ŞAHİNER, M., AKMAN, Ö., CORTEZ, M. A., SNEAD, O. C., İDRİZOĞLU, M. G. (2022). Pharmacologically induced absence seizures versus kindling in Wistar rats. İstanbul Kuzey Klinikleri, Cilt: 7 - Sayı: 1, 25–34. https://ajindex.com/articles/551439

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