KaydedilenlerDerginizi ekleyin

Dergiler / Harran Üniversitesi Veteriner Fakültesi Dergisi / 2021 / Cilt: 10 - Sayı: 2

Identification of Vibrio parahaemolyticus by PCR Method in Fish and WaterSamples from Tigris River, Turkey

Dicle Nehrindeki Balık ve Su Örneklerinde Vibrio parahaemolyticus’un PCR Yöntemi ile İdentifikasyonu

Muzaffer Aydın KETANİ
Filiz ÖZCAN
Neval Berrin ARSERİM
Sayfa
191–195
Yayın Tarihi
2022-10-18
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

According to the results, V. parahaemolyticus was detected in 40 (6.6%) fish samplestaken from the Seyrantepe region.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Climate change has caused increasing Vibrio parahaemolyticus infections in areas unaffected by this pathogen.Water temperature is an important ecological factor.
📊
Temel Bulgular & Veri:Total 480 samples of water and fish were used for V. parahaemolyticus detection.
💡
Sonuç & Katkı:According to the results, V. parahaemolyticus was detected in 40 (6.6%) fish samplestaken from the Seyrantepe region.
🔬Tür:Araştırma Makalesi
📍Kapsam:480 samples
⚙️Değişkenler:Alan Parametreleri

Özet

Climate change has caused increasing Vibrio parahaemolyticus infections in areas unaffected by this pathogen.Water temperature is an important ecological factor. It is consequently of high importance to follow V. parahaemolyticus inaquatic environments and foods. The aim of this work was to detect the presence of V. parahaemolyticus in Tigris Riverfrom eight different sites namely Malabadi Bridge, Fiskaya, Ongözlü Bridge, Bağıvar, Pamukçay, Ambarçay, Seyrantepe andSilvan. Total 480 samples of water and fish were used for V. parahaemolyticus detection. Thiosulphatecitrate‐bile saltsucrose (TCBS) Agar was employed for bacteria isolation for 24 h at 37◦C. The dominant uniform bacterial colonies werepurified by streaking onto the TCBS agar plates three times. Identification of this pathogen was made by PCR. A total of 480water and fish samples were analyzed. According to the results, V. parahaemolyticus was detected in 40 (6.6%) fish samplestaken from the Seyrantepe region.

Abstract

İklim değişikliğinin bir sonucu olarak artan sayıda Vibrio parahaemolyticus enfeksiyonu tespit edilmiştir. Su sıcaklığıçok güçlü bir ekolojik faktördür. Dolayısıyla su ortamlarında ve su ürünlerinde V. parahaemolyticus'u takip etmek önemarzetmektedir. Bu çalışmanın amacı, Dicle nehrinde bulunan Malabadi Köprüsü, Fiskaya, Ongözlü Köprüsü, Bağıvar,Pamukçay, Ambarçay, Seyrantepe ve Silvan olmak üzere sekiz farklı yerdeki Vibrio parahaemolyticus'un varlığınıaraştırmaktır. Bu amaçla toplam 480 su ve balık numunesi kullanılmıştır. Bakteri izolasyonu için örnekler Tiyosülfatsitrat‐ safra tuzu sukroz (TCBS) Agara ekim yapılmış, 37°C'de 24 saat inkübe edilmiştir ve bakteri kolonileri, TCBS agar plakalarınaüç kez sürme yoluyla saflaştırılmıştır. Patojenin kesin tespiti PCR testi ile gerçekleştirilmiştir. Araştırmanın sonucundaSeyrantepe'deki 40 (6.6%) adet balık örneğinde patojenin varlığı tespit edilmiştir.

🔖

Bu Makaleye Atıf Yap

KETANİ, M. A., ÖZCAN, F., ARSERİM, N. B. (2022). Identification of Vibrio parahaemolyticus by PCR Method in Fish and WaterSamples from Tigris River, Turkey. Harran Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 10 - Sayı: 2, 191–195. https://ajindex.com/articles/534190

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (4)

  1. Ahmed HA, Abdelazim RS, Gharieb RMA, Abou Elez RMM, Awadallah, MAI 2018: Prevalence of antıbıotıc resıstant v. parahaemolyticus and v. cholerae ın fısh and humans wıth specıal reference to vırulotypıng and genotypıng of V. Parahaemolyticus Slov Vet Res 55 (Suppl 20): 251– 62 Adesiyan MI, Bisi‐Johnson MA, Ogunfowokan A.O, Okoh AI 2021: Occurrence and antibiogram signatures of some Vibrio species recovered from selected rivers in South West Nigeria Environmental Science and Pollution Research 28:42458–42476 Al‐Murib M, Wells S, Talke SA 2017: Estimation of Surface Water Temperature of the Tigris River System in Iraq World Environmental and Water Resources Congress. Babai J, Kıra R, Mohd Sınang F, Maurıce Bılung L. 2020: Occurrences of Vibrio parahaemolyticus in Retailed Freshwater Fish Trends in Undergraduate Research, 3: a1‐4. Constantin de Magny G, Long W, Brown C, Hood R, Huq A, Murtugudde R, Colwell R 2009: Predicting the distribution of Vibrio spp. in the Chesapeake Bay: a Vibrio cholerae case study. Eco Health, 6, 378–389.
  2. DePaola A, Nordstrom JL, Bowers JC, Wells J.G, Cook DW 2003: Seasonal abundance of total and pathogenic Vibrio parahaemolyticus in Alabama oysters. Appl Environ Microbiol 69, 1521–1526. European Environmental Agency Climate Change,Impactsand Vulnerability in Europe Copenhagen, Denmark:European Environmental Agency, 2012.
  3. Federici S, Serrazanetti DI, Elisabetta Guerzoni M, Campana R, Ciandrini E, Baffone W, Gianott, 2018: A. Development of a rapid PCR protocol to detect Vibrio parahaemolyticus in clams, J Food Sci Technol, 55(2):749–759
  4. Froelich BA, Daines DA 2020: In hot water: Effects of climate change on Vibrio–human interactions, Environmental Microbiology, 22(10), 4101–4111. Gayathri,V. 2011: The prevalence and public health significance of human pathogenic Vibrio species in Hawai’i’s diverse tropical coastal water environments. Water Resources Research Centre, University of Hawaii, Guin S, Saravanan MA, Chowdhury G, Perumal Pazhani G, Ramamurthy T, Chandra SD. 2019: Pathogenic Vibrio parahaemolyticus indiarrhoeal patients, fish and aquatic environments and their potential for inter‐ source transmission Heliyon, 5. e01743 Givens CE, Bowers JC, DePaola A, Hollibaugh JT, Jones JL 2014: Occurrence and distribution of Vibrio vulnificus and Vibrio parahaemolyticus – potential roles for fish, oyster,sediment and water. Letters in Applied Microbiology,58,503—510. Hemmat M Ibrahim, Reham A, Amin 1, Nesreen Z, Eleiwa Hanan R, Ghanaym M. 2