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Dergiler / Cumhuriyet Science Journal / 2021 / Cilt: 42 - Sayı: 4

Electrochemical investigation of DNA and Capecitabine interaction using glassy carbon electrode (GCE)

Derya KIZILOLUK
Gültekin GÖKÇE
Şenay ÇETİNUS
Sayfa
814–821
Yayın Tarihi
2022-10-12
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Detection limit (DL) was found as 17.54 μg / mL for interaction capecitabine with ds-DNA and was found 17.12 μg / mL for interaction capecitabine with ss-DNA.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:In recent years, studies with biosensors have increased in order to better understand the mechanisms of anticancer drug action.
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Temel Bulgular & Veri:Thus, studies to examine interactions with DNA using biosensors have gained momentum.
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Sonuç & Katkı:Detection limit (DL) was found as 17.54 μg / mL for interaction capecitabine with ds-DNA and was found 17.12 μg / mL for interaction capecitabine with ss-DNA.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

In recent years, studies with biosensors have increased in order to better understand the mechanisms of anticancer drug action. Thus, studies to examine interactions with DNA using biosensors have gained momentum. In our study, it was investigated that Capecitabine (CPT), an anticancer drug, and glassy carbon electrode (GCE) interaction by using electrochemical methods. The interaction of CPT with calf thymus DNAs (dsDNA, ssDNA) immobilized on the electrode surface was analyzed by exploiting changes in the oxidation signals of the guanine base. The immobilization of DNA on the electrode surface has been optimized. Optimal DNA concentration and optimal interaction times were found. Electrochemical impedance spectroscopy technique was used for impedimetric measurements. The results obtained confirmed that the ct-DNAs were immobilized on the electrode surface. Detection limit (DL) was found as 17.54 μg / mL for interaction capecitabine with ds-DNA and was found 17.12 μg / mL for interaction capecitabine with ss-DNA.

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Bu Makaleye Atıf Yap

KIZILOLUK, D., GÖKÇE, G., ÇETİNUS, Ş. (2022). Electrochemical investigation of DNA and Capecitabine interaction using glassy carbon electrode (GCE). Cumhuriyet Science Journal, Cilt: 42 - Sayı: 4, 814–821. https://ajindex.com/articles/477571

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