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Journals / Cumhuriyet Science Journal / 2021 / Cilt: 42 - Sayı: 4

Electrochemical investigation of DNA and Capecitabine interaction using glassy carbon electrode (GCE)

Derya KIZILOLUK
Gültekin GÖKÇE
Şenay ÇETİNUS
Pages
814–821
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Detection limit (DL) was found as 17.54 μg / mL for interaction capecitabine with ds-DNA and was found 17.12 μg / mL for interaction capecitabine with ss-DNA.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In recent years, studies with biosensors have increased in order to better understand the mechanisms of anticancer drug action.
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Key Findings & Data:Thus, studies to examine interactions with DNA using biosensors have gained momentum.
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Conclusion & Impact:Detection limit (DL) was found as 17.54 μg / mL for interaction capecitabine with ds-DNA and was found 17.12 μg / mL for interaction capecitabine with ss-DNA.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In recent years, studies with biosensors have increased in order to better understand the mechanisms of anticancer drug action. Thus, studies to examine interactions with DNA using biosensors have gained momentum. In our study, it was investigated that Capecitabine (CPT), an anticancer drug, and glassy carbon electrode (GCE) interaction by using electrochemical methods. The interaction of CPT with calf thymus DNAs (dsDNA, ssDNA) immobilized on the electrode surface was analyzed by exploiting changes in the oxidation signals of the guanine base. The immobilization of DNA on the electrode surface has been optimized. Optimal DNA concentration and optimal interaction times were found. Electrochemical impedance spectroscopy technique was used for impedimetric measurements. The results obtained confirmed that the ct-DNAs were immobilized on the electrode surface. Detection limit (DL) was found as 17.54 μg / mL for interaction capecitabine with ds-DNA and was found 17.12 μg / mL for interaction capecitabine with ss-DNA.

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Cite This Article

KIZILOLUK, D., GÖKÇE, G., ÇETİNUS, Ş. (2022). Electrochemical investigation of DNA and Capecitabine interaction using glassy carbon electrode (GCE). Cumhuriyet Science Journal, Cilt: 42 - Sayı: 4, 814–821. https://ajindex.com/articles/477571

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