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Dergiler / Avrupa Bilim ve Teknoloji Dergisi / 2021 / Sayı: 27

Optimization of a Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) Method for Simultaneous Separation of Aloe-Emodin, Rhein, Emodin, Chrysophanol and Physcion

Gizem YILDIRIM BAŞTEMUR
Reyhan AKPINAR
Sabriye PERÇİN ÖZKORUCUKLU
Esengül KIR
Sayfa
972–984
Yayın Tarihi
2021-11-30
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

This study showed an optimized analytical method can be effectively utilized to identify and measure anthraquinone compounds.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:In this study, a reverse-phase high performance liquid chromatography (RP-HPLC) method with high separation efficiency, high detection sensitivity and excellent selectivity was successfully developed for the simultaneous quantitative determination of the aloe-emodin, rhein, emodin, chrysophanol, and physcion compounds.
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Temel Bulgular & Veri:These optimum method conditions were determined by studying various columns, mobile phases and compositions, flow rates and column temperatures.
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Sonuç & Katkı:This study showed an optimized analytical method can be effectively utilized to identify and measure anthraquinone compounds.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Reverse Phase High Performance Liquid Chromatography · Anthraquinon · Secondary Metabolites

Özet

In this study, a reverse-phase high performance liquid chromatography (RP-HPLC) method with high separation efficiency, high detection sensitivity and excellent selectivity was successfully developed for the simultaneous quantitative determination of the aloe-emodin, rhein, emodin, chrysophanol, and physcion compounds. These optimum method conditions were determined by studying various columns, mobile phases and compositions, flow rates and column temperatures. The successful separation was carried out with a Supelcosil LC-18 column (250 × 4.6 mm, 5 µm) and a gradient program. The procedure was carried out at 20 °C with the flow rate of 1.0 mL/min and the injection volume of 20 μL utilizing an RP-HPLC method with DAD detector at 225 nm. Deionized water containing 0.5% orthophosphoric acid and methanol was used as the mobile phases A and B. This study showed an optimized analytical method can be effectively utilized to identify and measure anthraquinone compounds.

Abstract

In this study, a reverse-phase high performance liquid chromatography (RP-HPLC) method with high separation efficiency, high detection sensitivity and excellent selectivity was successfully developed for the simultaneous quantitative determination of the aloe-emodin, rhein, emodin, chrysophanol, and physcion compounds. These optimum method conditions were determined by studying various columns, mobile phases and compositions, flow rates and column temperatures. The successful separation was carried out with a Supelcosil LC-18 column (250 × 4.6 mm, 5 µm) and a gradient program. The procedure was carried out at 20 °C with the flow rate of 1.0 mL/min and the injection volume of 20 μL utilizing an RP-HPLC method with DAD detector at 225 nm. Deionized water containing 0.5% orthophosphoric acid and methanol was used as the mobile phases A and B. This study showed an optimized analytical method can be effectively utilized to identify and measure anthraquinone compounds.

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Bu Makaleye Atıf Yap

BAŞTEMUR, G. Y., AKPINAR, R., ÖZKORUCUKLU, S. P., KIR, E. (2021). Optimization of a Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) Method for Simultaneous Separation of Aloe-Emodin, Rhein, Emodin, Chrysophanol and Physcion. Avrupa Bilim ve Teknoloji Dergisi, Sayı: 27, 972–984. https://ajindex.com/articles/470808

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Kaynakça (3)

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  2. Ahmad, W., Zaidi, S.M.A., Mujeeb, M., Ansari, S.H., & Ahmad, S., (2014). HPLC and HPTLC Methods by Design for Quantitative Characterization and in vitro Anti-oxidant Activity of Polyherbal Formulation Containing Rheum emodi. Journal of Chromatographic Science, 52 (8), 911-918. doi: 10.1093/chromsci/bmt123 Aichner, D., & Ganzera, M., (2015). Analysis of anthraquinones in rhubarb (Rheum palmatum and Rheum officinale) by supercritical fluid chromatography. Talanta, 144, 1239-1244. doi: 10.1016/j.talanta.2015.08.011 Chen, C., Fu, Z., Zhou, W., Chen, Q., Wang, C., Xu, L., Wang, Z. and Zhang, H., (2020). Ionic liquid-immobilized NaY zeolite-based matrix solid phase dispersion for the extraction of active constituents in Rheum palmatum L. Microchemical Journal, 152, 104245. doi: 10.1016/j.microc.2019.104245 Chen, S.H., Lin, K.Y., Chang, C.C., Fang, C.L., & Lin, C.P., (2007). Aloe-emodin-induced apoptosis in human gastric carcinoma cells. Food and Chemical Toxicology, 45 (11), 2296-2303. doi: 10.1016/j.fct.2007.06.005 Chien, S.C., Wu, Y.C., Chen, Z.W., & Yang, W.C., (2015). Naturally occurring anthraquinones: chemistry and therapeutic potential in autoimmune diabetes. Evidence-Based Complementary and Alternative Medicine, Article ID 357357. doi: 10.1155/2015/357357 ElSohly, M.A., Gul, W., & Murphy, T.P., (2004). Analysis of the anthraquinones aloe-emodin and aloin by gas chromatography/mass spectrometry. International Immunopharmacology, 4 (14), 1739-1744. doi: 10.1016/j.intimp.2004.07.005 Farooq, U., Pandith, S.A., Singh, Saggoo, M.I., & Lattoo, S.K., (2013). Altitudinal variability in anthraquinone constituents from novel cytotypes of Rumex nepalensis Spreng—a high value medicinal herb of North Western Himalayas. Industrial Crops and Products, 50, 112-117. doi: 10.1016/j.indcrop.2013.06.044 Feng, S.X., Li, M.M., Zhao, D., Li, X.H., Zhang, L., Wang, Z., & Gao, N.N., (2017). Simultaneous Determination of 10 Anthraquinones in Rhubarb Based on HPLC-Q-HR/MS. Chinese Herbal Medicines, 9 (4), 388-395. doi: 10.1016/S1674-6384(17)60120-5
  3. Gao, X.Y., Jiang, Y., Lu, J., & Tu, P-.F., (2009). One single standard substance for the determination of multiple anthraquinone derivatives in rhubarb using high-performance liquid chromatography-diode array detection. Journal of C