KaydedilenlerDerginizi ekleyin

Dergiler / Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi / 2017 / Cilt: 17 - Sayı: 1

In Vitro Cytotoxicity and Molecular Effects Related to Silicon Nanoparticles Exposures

Elanur AYDIN
Hasan TÜRKEZ
Fazıl HACIMÜFTÜOĞLU
Sayfa
10–17
Yayın Tarihi
2017-04-24
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

This study of gene expression profiles affected by nanotoxicity provides critical information for the clinical and environmental applications of silicon nanoparticles.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Silicon nanoparticles are widely used for various applications including environmental, biological, chemical and physical.
📊
Temel Bulgular & Veri:HPAEpiC and HPPC cells were treated with silicon (0-100 μg/mL), nanoparticles for 72 h, and then cytotoxicity was detected by, [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT) and lactate dehydrogenase (LDH) release assays, while genotoxicity was also analyzed by cDNA array - RT-PCR assay.
💡
Sonuç & Katkı:This study of gene expression profiles affected by nanotoxicity provides critical information for the clinical and environmental applications of silicon nanoparticles.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Airway epithelial cell · Genotoxicity In vitro gene expression Nanotoxicity Silicon nanoparticles

Özet

Silicon nanoparticles are widely used for various applications including environmental, biological, chemical and physical. And, to translate these nanomaterials to the clinic and industrial domains, their safety needs to be verified, particularly in terms of genotoxicity and cytotoxicity. Therefore, in this study, we aimed to investigate of cytotoxicity and changes in gene expression profiles influenced by commonly silicon (as silicon carbide, silicon dioxide, silicon nitride) nanoparticles in human alveolar epithelial (HPAEpiC) and pharynx (HPPC) cell lines in vitro since inhalation is an important pathway for exposure to these nanoparticles. HPAEpiC and HPPC cells were treated with silicon (0-100 μg/mL), nanoparticles for 72 h, and then cytotoxicity was detected by, [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT) and lactate dehydrogenase (LDH) release assays, while genotoxicity was also analyzed by cDNA array - RT-PCR assay. According to the results of MTT and LDH assays, all tested nanoparticles induced cytotoxicity on both HPAEpiC and HPPC cells in dose-dependent manner. Determining and analyzing the gene expression profiles of HPAEpiC and HPPC cells, silicon nanoparticles showed changes in genes related to apoptosis, DNA damage or repair and oxidative stress. This study of gene expression profiles affected by nanotoxicity provides critical information for the clinical and environmental applications of silicon nanoparticles.

🔖

Bu Makaleye Atıf Yap

AYDIN, E., TÜRKEZ, H., HACIMÜFTÜOĞLU, F. (2017). In Vitro Cytotoxicity and Molecular Effects Related to Silicon Nanoparticles Exposures. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, Cilt: 17 - Sayı: 1, 10–17. https://ajindex.com/articles/451524

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (9)

  1. Amoabediny, Gh., Naderi, A., Malakootikhah, J., Koohi, MK., M ortazavi, S.A., N aderi, M. a nd R ashedi, H., 2009. Guidelines for safe handling, use and disposal of nanoparticles. Journal of Physics: Conference Series, 170, 012037.
  2. Asadpour, E., Sadeghnia, H.R., Ghorbani, A., Sedaghat, M. and Boroushaki, M.T., 2016. Oxidative stressmediated cytotoxicity of zirconia nanoparticles on PC12 and N2a cells. Journal of Nanoparticle Research, 18, 14, (in press) DOI 10.1007/s11051-015-3316-7.
  3. Baca, A.J., Meitl, M.A., Ko, H.C., Mack, S., Kim, H.S., Dong, J.Y., Ferreira, P.M. and Rogers, J.A., 2007. Printable single-crystal silicon micro/nanoscale ribbons, platelets and bars generated from bulk wafers. Advanced Functional Materials, 17, 3051- 3062. Brown, D.R., Herms, J. and Kretzschmar, H.A., 1994. Mouse cortical cells lacking cellular PrP survive in culture with a neurotoxic PrP fragment. Neuroreport, 5, 2057-2060.
  4. Cavarroc, M., Mikikian, M., Perrier, G. and Boufendi, L., 2006. Single-crystal silicon nanoparticles: An instability to check their synthesis. Applied Physics Letters, 89, 013107 Chantrenne, P. and Lysenko, V., 2005. Thermal conductivity of interconnected silicon nanoparticles: Application to porous silicon nanostructures. Physical Review B, 72, 035318. Chen, Y., Chen, J., Dong, J. and Jin, Y., 2004. Comparing study of the effect of nanosized silicon dioxide and microsized silicon dioxide on fibrogenesis in rats. Toxicology Industrial Health, 20, 21-27.
  5. Chen, Z., Meng, H., Xing, G., Chen, C., Zhao, Y., Jia, G., Wang, T., Yuan, H., Ye, C., Zhao, F., Chai, Z., Zhu, C., Fang, X., Ma, B. and Wan, L., 2006. Acute toxicological effects of copper nanoparticles in vivo. Toxicology Letters, 163, 109-120.
  6. Choi, J., Zhang, Q., Reipa, V., Wang, N.S., Stratmeyer, M.E., Hitchins, V.M. and Goering, P.L., 2009. Comparison of cytotoxic and inflammatory responses of photoluminescent silicon nanoparticles with silicon micron-sized particles in RAW 264.7 macrophages. Journal of Applied Toxicology, 29, 52-60.
  7. Fischer, H.C. and Chan, W.C., 2007. Nanotoxicity: the growing need for in vivo study. Current Opinion in Biotechnology, 18, 565-571.
  8. Gerloff, K., Albrecht, C., Boots, A.W., Förster, I. and Schins, R.P.F., 2009. Cytotoxicity and oxidative DNA damage by nanoparticles in human intestinal Caco-2 cells. Nanotoxicology, 3, 355-364.
  9. Gök, H., 2007. Nanotechnology: future directions from physiatrists’ perspective. The Turkish Journal of Physical Medicine and Rehabilitation,