KaydedilenlerDerginizi ekleyin

Dergiler / Turkish Journal of Pediatrics / 2019 / Cilt: 61 - Sayı: 6

Thymic output changes in children with clinical findings signaling a probable primary immunodeficiency

Neslihan KARACA
Elif AZARSIZ
Sanem Eren AKARCAN
Güzide AKSU
Necil KÜTÜKÇÜLER
Sayfa
885–894
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Naive T-cells (p: 0.037) and RTE cells(p: 0.032) were also lower in patients who had cardiac surgery in the past.In conclusion, flow cytometric CD31+thymic naive RTE cell evaluation mayprovide rapid clinical information especially on T-cell immune dysfunctionand CD4+CD45RA+CD31+ RTE cells may be used as an alternative to TRECsin the diagnosis of combined immunodeficiencies.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Thymic maturation evaluation is inevitable for patients with clinicaland laboratory findings for a primary immunodeficiency, as the T cellimmunodeficiencies are the most severe type.
📊
Temel Bulgular & Veri:In this study, we aimed to showthe usage of T cell surface molecule “CD31” for the evaluation of thymicoutput in patients (n: 66) with a large spectrum of findings signing a probableprimary immunodeficiency.
💡
Sonuç & Katkı:Naive T-cells (p: 0.037) and RTE cells(p: 0.032) were also lower in patients who had cardiac surgery in the past.In conclusion, flow cytometric CD31+thymic naive RTE cell evaluation mayprovide rapid clinical information especially on T-cell immune dysfunctionand CD4+CD45RA+CD31+ RTE cells may be used as an alternative to TRECsin the diagnosis of combined immunodeficiencies.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

Thymic maturation evaluation is inevitable for patients with clinicaland laboratory findings for a primary immunodeficiency, as the T cellimmunodeficiencies are the most severe type. In this study, we aimed to showthe usage of T cell surface molecule “CD31” for the evaluation of thymicoutput in patients (n: 66) with a large spectrum of findings signing a probableprimary immunodeficiency. Besides the classical clinical and laboratoryapproach for these patients, T cell subpopulations as naive, memory, recentthymic emigrant cells were also investigated. The humoral immunodeficiency(34.8%), combined immunodeficiency (34.8%) and cardiopathy (7.6%) werethe most frequent diagnosis groups. CD4+CD45RA+ naive T-cells percentages(p: 0.011) and absolute counts (p: 0.004) and absolute CD4+CD45RA+CD31+RTE (recent thymic emigrant) cell counts (p: 0.007) were significantly lowerin combined immunodeficiency group. Naive T-cells (p: 0.037) and RTE cells(p: 0.032) were also lower in patients who had cardiac surgery in the past.In conclusion, flow cytometric CD31+thymic naive RTE cell evaluation mayprovide rapid clinical information especially on T-cell immune dysfunctionand CD4+CD45RA+CD31+ RTE cells may be used as an alternative to TRECsin the diagnosis of combined immunodeficiencies.

🔖

Bu Makaleye Atıf Yap

KARACA, N., AZARSIZ, E., AKARCAN, S. E., AKSU, G., KÜTÜKÇÜLER, N. (2019). Thymic output changes in children with clinical findings signaling a probable primary immunodeficiency. Turkish Journal of Pediatrics, Cilt: 61 - Sayı: 6, 885–894. https://ajindex.com/articles/412001

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (14)

  1. Kimmig S, Przybylski GK, Schmidt CA, et al. Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood. J Exp Med 2002; 195: 789-794
  2. Boehm T, Swann JB. Thymus involution and regeneration: two sides of the same coin? Nat Rev Immunol 2013; 13: 831-838
  3. Harris JM, Hazenberg MD, Poulin JF, et al. Multiparameter evaluation of human thymic function: interpretations and caveats. Clin Immunol 2005; 115: 138-146
  4. Hazenberg MD, Verschuren MC, Hamann D, Miedema F, van Dongen JJ. T cell receptor excision circles as markers for recent thymic emigrants: basic aspects, technical approach, and guidelines for interpretation. J Mol Med (Berl) 2001; 79: 631-640
  5. De Rosa SC, Herzenberg LA, Herzenberg LA, Roederer M. 11-color, 13-parameter flow cytometry: identification of human naive T cells by phenotype, function, and T-cell receptor diversity. Nat Med 2001; 7: 245-248
  6. Kohler S, Thiel A. Life after the thymus: CD31+ and CD31- human naive CD4+ T-cell subsets. Blood 2009; 113: 769-774
  7. Junge S, Kloeckener-Gruissem B, Zufferey R, et al. Correlation between recent thymic emigrants and CD31+ (PECAM-1) CD4+ T cells in normal individuals during aging and in lymphopenic children. Eur J Immunol 2007; 37: 3270-3280
  8. Stockinger H, Schreiber W, Majdic O, Holter W, Maurer D, Knapp W. Phenotype of human T cells expressing CD31, a molecule of the immunoglobulin supergene family. Immunology 1992; 75: 53-58
  9. Kohler S, Wagner U, Pierer M, et al. Post-thymic in vivo proliferation of naive CD4+ T cells constrains the TCR repertoire in healthy human adults. Eur J Immunol 2005; 35: 1987-1994
  10. Isgro A, Marziali M, Mezzaroma I, et al. Bone marrow clonogenic capability, cytokine production, and thymic output in patients with common variable immunodeficiency. J Immunol 2005; 174: 5074-5081
  11. Duszczyszyn DA, Beck JD, Antel J, et al. Altered naive CD4 and CD8 T cell homeostasis in patients with relapsing-remitting multiple sclerosis: thymic versus peripheral (non-thymic) mechanisms. Clin Exp Immunol 2006; 143: 305-313
  12. Greinix HT, Kuzmina Z, Weigl R, et al. CD19+CD21low B cells and CD4+CD45RA+CD31+T cells correlate with first diagnosis of chronic graft-versus-host disease. Biol Blood Marrow Transplant 2015; 21: 250-258
  13. Armengol MP, Sabater L, Fernandez M, et al. Influx of recent thymic emigrants into autoimmune thyroid disease glands in humans. Clin Exp Immunol 2008; 153: 338-350
  14. Douek DC, M