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Dergiler / Turkish Journal of Pharmaceutical Sciences / 2021 / Cilt: 18 - Sayı: 2

Comparative Proteomic Analysis of Escherichia coli Under Ofloxacin Stress

Ofloksasin Stresi Altında Escherichia coli’nin Karşılaştırmalı Proteomik Analizi

Engin KOÇAK
Ceren KOÇAK
Sayfa
21–26
DOI
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In addition, proteomic analysis revealed that E. coli develops resistance mechanism with different biological processes

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Amaç & Hipotez:Objectives: In the present study, proteomics was utilized to evaluate changes in Escherichia coli proteins in response to ofloxacin to understand the mechanism of action of ofloxacin and the mechanisms of ofloxacin resistance in E. coli.
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Temel Bulgular & Veri:Results: In total, 649 common proteins were identified in the untreated and ofloxacin-treated groups, while 98 proteins were significantly different in the ofloxacin-treated group.
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Sonuç & Katkı:In addition, proteomic analysis revealed that E. coli develops resistance mechanism with different biological processes
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

Objectives: In the present study, proteomics was utilized to evaluate changes in Escherichia coli proteins in response to ofloxacin to understand the mechanism of action of ofloxacin and the mechanisms of ofloxacin resistance in E. coli. Materials and Methods: Proteomics analysis of E. coli was performed by using liquid chromatography quadrupole time-of-flight mass spectrometry followed by a data processing step using MaxQuant. Functional classification and pathway analysis showed a systematic effect of ofloxacin over E. coli proteome structure. Results: In total, 649 common proteins were identified in the untreated and ofloxacin-treated groups, while 98 proteins were significantly different in the ofloxacin-treated group. Functional classification and pathway analysis showed that ofloxacin has a systematic effect over ribosomal processes, energy pathways (tricarboxylic acid cycle and glycolysis), membrane proteins, microbial targets, and biofilm formation. Conclusion: The results showed that ofloxacin affected many cellular processes and pathways. In addition, proteomic analysis revealed that E. coli develops resistance mechanism with different biological processes

Abstract

Amaç: Bu çalışmada ofloksasinin etki mekanizmasına karşı yanıt olarak Escherichia coli proteinlerinde görülen değikliklerin belirlenmesi ve E. coli’deki oflotoksin direnç mekanizmalarının değerlendirilmesi için proteomiks kullanılmıştır. Gereç ve Yöntemler: E. coli’nin proteomik analizi sıvı kromatografisi/kuadrupol uçuş zamanlı kütle spektroskopisi kullanarak gerçekleştirilmiş ve sonrasında veri prosesleme aşaması MaxQuant kullanarak yapılmıştır. İşlevsel sınıflandırma ve yolak analizi oflotoksinin E. coli’nin proteome yapısına sistematik bir etkisinin olduğunu göstermiştir. Bulgular: Çalışmada 649 tane sıklıkla rastlanan proteinin yapısı oflotoksin ile muamele edilen ve edilmeyen gruplarda belirlenirken, 98 proteinin oflotoksin ile muamele gören grupta belirgin derecede farklı olduğunu göstermiştir. İşlevsel sınıflandırma ve yolak analizi oflotoksinin ribosomal prosesler, enerji yolakları (trikarboksilik asit siklusu ve glikoliz), membran proteinleri, mikrobiyal hedefler ve biofilm oluşumu üzerine sistematik bir etkisinin olduğunu göstermiştir. Sonuç: Bu sonuçlar ofloksasinin birçok hücresel prosesi ve yolağı etkilediğini göstermiştir. Ayrıca, proteomik analizler E. coli’nin farklı biyolojik prosesler ile direnç mekanizması geliştirdiğini göstermiştir.

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Bu Makaleye Atıf Yap

KOÇAK, E., KOÇAK, C. (2021). Comparative Proteomic Analysis of Escherichia coli Under Ofloxacin Stress. Turkish Journal of Pharmaceutical Sciences, Cilt: 18 - Sayı: 2, 21–26. https://ajindex.com/articles/404069

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Kaynakça (4)

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