Aqueous particulate matter (PM2.5) from Brazil alters antioxidant profile responses and causes oxidative stress

In vitro toxicity of the PM2.5 in different tissues, presented an ability tointeract with cellular antioxidant defense system, causing depletion of the sulfhydryl stocks, resulting in thedamage of biological molecules and inducing oxidative imbalance.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:In vitro assays were used to determine the effects of aqueous fine particulate matter (PM2.5) extracts on differenthomogenates of rat organs.

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Temel Bulgular & Veri:The PM2.5 collected from two regions (Seropédica and Duque de Caxias) in Rio deJaneiro, Brazil, were used to evaluate different oxidative damages and redox state.

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Sonuç & Katkı:In vitro toxicity of the PM2.5 in different tissues, presented an ability tointeract with cellular antioxidant defense system, causing depletion of the sulfhydryl stocks, resulting in thedamage of biological molecules and inducing oxidative imbalance.

🔬Tür:Araştırma Makalesi

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Alan Parametreleri

Özet

In vitro assays were used to determine the effects of aqueous fine particulate matter (PM2.5) extracts on differenthomogenates of rat organs. The PM2.5 collected from two regions (Seropédica and Duque de Caxias) in Rio deJaneiro, Brazil, were used to evaluate different oxidative damages and redox state. Two PM2.5 extract concentrations (concentrate and 10x dilution) were evaluated using rat tissue homogenates (heart, lung, liver, andkidney). Measurements of total antioxidant capacity (ACAP), lipid peroxidation levels (LPO), reduced glutathione concentrations (GSH), activity of glutamate cysteine ligase (GCL), activity of glutathione S-transferase(GST) and catalase (CAT) were performed. Most PM2.5 aqueous extract concentrations contain numerous metals(e.g. Li, Ti, Fe, Cu, Cd, Sr, among others), which reduced ACAP and generated LPO in most tissue homogenates.Besides this, GST activity decreased in liver and lung, increasing in kidney and heart homogenates. GCL activityincreased in lung, liver and kidney and undergo decrease in the heart homogenate. All concentrations of PM2.5evaluated decreased GSH levels. PM2.5 concentrations for both sites (regions) raised catalase activity in mosttissue homogenates except in the liver. In vitro toxicity of the PM2.5 in different tissues, presented an ability tointeract with cellular antioxidant defense system, causing depletion of the sulfhydryl stocks, resulting in thedamage of biological molecules and inducing oxidative imbalance.

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Bu Makaleye Atıf Yap

RIBEIRO, J. D. P., QUIJANO, M. F. C. C., FERREIRO, J. D., GIODA, A., JIMENEZ-VELEZ, B., MONSERRAT, J. M., GIODA, C. R. (2020). Aqueous particulate matter (PM2.5) from Brazil alters antioxidant profile responses and causes oxidative stress. Atmospheric Pollution Research, Cilt: 11 - Sayı: 3, 511–519. https://ajindex.com/articles/351436

Kaynakça (5)

Amado, L.L., Garcia, M.L., Ramos, P.B., Freitas, R.F., Zafalon, B., Ferreira, J.L.R., Yunes, J.S., Monserrat, J.M., 2009. A method to measure total antioxidant capacity against peroxyl radicals in aquatic organisms: application to evaluate microcystins toxicity. Sci. Total Environ. 407, 2115–2123. https://doi.org/10.1016/j.scitotenv.2008.11. 038. Andre, L., Boissie, J., Reboul, C., Perrier, R., Zalvidea, S., Meyer, G., Thireau, J., Tanguy, S., Bideaux, P., Hayot, M., Boucher, F., Obert, P., Cazorla, O., Sylvain, R., 2010. Carbon monoxide pollution promotes cardiac remodeling and ventricular arrhythmia in healthy rats. Am. J. Respir. Crit. Care Med. 181, 587–595. https://doi.org/10. 1164/rccm.200905-0794OC. Anwar, M., Abdelhalim, K., Jarrar, B.M., 2011. Gold nanoparticles administration induced prominent inflammatory, central vein intima disruption, fatty change and Kupffer cells hyperplasia. Lipids Health Dis. 10, 133. https://doi.org/10.1186/1476- 511X-10-133.
Araujo, J.A., Barajas, B., Kleinman, M., Wang, X., Bennett, B.J., Gong, K.W., Navab, M., Harkema, J., Sioutas, C., Lusis, A.J., Nel, A.E., 2008. Ambient particulate pollutants in the ultrafine range promote early atherosclerosis and systemic oxidative stress. Circ. Res. 102, 589–596. https://doi.org/10.1161/CIRCRESAHA.107.164970.
Aztatzi-Aguilar, O.G., Uribe-Ramírez, M., Narváez-Morales, J., De Vizcaya-Ruiz, A., Barbier, O., 2016. Early kidney damage induced by subchronic exposure toPM2.5 in in rats. Part. Fibre Toxicol. 13, 1–20. https://doi.org/10.1186/s12989-016-0179-8.
Ballatori, N., Krance, S.M., Notenboom, S., Shi, S., Tieu, K., Hammond, C.L., 2009. Glutathione dysregulation and the etiology and progression of human diseases. Biol. Chem. 390, 191–214. https://doi.org/10.1515/BC.2009.033.
Baskaran, R., Priya, L.B., Sathish Kumar, V., Padma, V.V., 2017. Tinospora cordifolia extract prevents cadmium-induced oxidative stress and hepatotoxicity in experimental rats. J. Ayurveda Integr. Med. 1–6. https://doi.org/10.1016/j.jaim.2017.07. 005. xxx. Biswas, S.K., Rahman, I., 2009. Environmental toxicity, redox signaling and lung inflammation: the role of glutathione. Mol. Asp. Med. 30, 60–76. https://doi.org/10. 1016/j.mam.2008.07.001. Britto, R.S., Garcia, M.L., Martins da Rocha, A., Flores, J.A., Pinheiro, M.V.B., Monserrat, J.M., Ferreira, J.L.R., 2012. Effects of carbon nanomaterials fullerene C 60 and fullerol C 60(OH) 18-22 on gills of fish Cyprinus carpio (Cyprinidae) exposed to ultraviolet radia