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AI InsightBu durum aynı zamanda erken dönemde serum PSA seviyelerini de etkilemektedir.
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Özet
ÖZET Bu çalışmamızda ,akut alkol alımının hem prostat dokusu üzerinde meydana getirdiği olası değişikliklerin ultrastrüktürel düzeyde incelenmesi hem de serum prostat spesifik antijen seviyeleri üzerindeki etkisi incelemeyi amaçladık. Kırkdört Wistar erkek rat dört gruba ayrıldı; her grupta 7 tedavi ve 4 kontrol hayvanı vardı. Tedavi grubuna 40’lık etanol, kontrol grubuna ise izotonik salin enjeksiyonu uygulandı. Birinci grup 3., 2.grup 24., 3. grup 72. saat ve 4. grup 7. gün sakrifiye edilerek ventral prostat lobları çıkartıldı. Alınan doku örnekleri ışık ve elektron mikroskobunda incelemeye alındı. Tüm ratlardan tedavi öncesi ve sonrası sakrifikasyondan hemen önce PSA analizi için kan örneği alındı. 1-3 gruplarda alkol tedavi sonrasında ışık mikroskobisinde kesitlerde artmış konjesyon ve ödem gözlenirken, elektron mikroskobisinde prostatik asiner hücrelerin endoplazmik retikulum sisternalarında dilatasyon, golgi komplekslerinde düzensizleşmeler ve bazal labirente ait açılmalar izlendi. Ayrıca asinus lümeni içerisinde yoğun döküntü hücreleri ve epitelin bazal membrandan ayrıştığı gözlendi. Birinci gruptaki alkol ratlarında tedavi öncesi ve sonrası değerlere bakıldığında serum PSA düzeyinde izlenen artış %36.8 olarak belirlendi ve bu artış diğer gruplarla karşılaştırıldığında istatistiksel olarak anlamlı bulundu (p=0.006). Akut alkol’ün prostat dokusu üzerinde gösterdiği esas etki hücrenin organel düzeyinde egzokrin salgılama işlevinde rol alan ara basamaklarında da gözlenmiştir. Bu durum aynı zamanda erken dönemde serum PSA seviyelerini de etkilemektedir.
Abstract
Introduction: In this study, we aimed to investigate both the possible changes induced by acute alcohol intake on prostate tissue at the ultrastructural level and its effect on serum prostate specific antigen (PSA)-like protein levels in rats. Materials and Method: The study included a total of 44 Wistar male rats, an average weight of 180 grams (range ± SD, 150-210 ± 35) and at least 3.5 months of age. Rats were divided into four groups. For each group, 7 alcohol and 4 control animals were selected. The treatment group received 40% ethanol (6gr/kg) while the control group received same dose of intra-peritoneal isotonic saline injection. One ml blood sample was taken from the tail vein of each rat prior to the injection. The first group was sacrificed in 3 hours, the second group in 24 hours, the third group in 72 hours and the fourth group in day 7. Following the sacrification, at least 1 ml intra-cardiac blood samples were immediately taken from each rat and placed into dry tubes, and after separating their serums, they were kept under – 80°C. Samples were examined by light and Jeol-100 electron microscope. Total serum PSA-like protein levels were determined by Tosoh immunoenzymometric analyzer AIA 600. Pre- and post-alcohol total PSA-like protein values were statistically analyzed by Mann-Whitney and Wilcoxon signed Ranks Test for all groups. Results: In groups 1-3, electron microscopy showed dilatations in the endoplasmic reticulum cisternae of prostatic aciner cells, disarrangements in apparent Golgi complex and apertures belonging to basal labyrinth. It was also observed that there was a mass of debris cells inside the aciner lumen, and secretory epithelium was detached from basal membrane in some places. No pathology was found in group 4 in light and electron microscopy. In group 1, significant levels of increased serum total PSA-like protein was found compared to controls and other treatment groups (p=0.006). When pre- and post-treatment values of alcohol rats in the first group were considered, the increase in serum PSA-like protein levels was found 36.8%, and this increase was statistically significant compared to other groups (p=0.018). In other groups, no statistically significant difference was found in serum PSA-like protein levels between alcohol and control rats. Conclusion: The fundamental effect of acute alcohol intake on prostate tissue was observed in intermediate stages participating to the exocrine secretion process in the cellular organelles. This experimental study indicates that early PSA assessments following alcohol intake might mistakenly result in high values, and notifies the clinical practice.