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AI InsightIt was also observed that human-IgG could be repeatedly adsorbed and desorbed with p HEMA-VPBA microbeads without significant loss in the adsorption capacity.
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Abstract
In this study a novel approach was developed to obtain an efficient separation of human-IgG from human plasma. 4- Vinylphenylboronic acid VPBA and 2-hydroxyethylmethacrylate HEMA were chosen as monomers. Spherical microbeads with an average size of 100-140 µm were obtained by the radical suspension polymerization of VPBA and HEMA conducted in an aqueous dispersion medium. VPBA monomer was characterized by FTIR. p HEMA-VPBA beads were characterized by swelling studies, FTIR and SEM. The pHEMA and p HEMA-VPBA microbeads were contacted with blood in in-vitro systems. Loss of the blood cells and the clotting times were also followed. Good blood compatibility properties of both pHEMA and p HEMA-VPBA were clearly observed with blood coagulation experiments. Loss of cells in the blood contacting with pHEMA and p HEMA-VPBA microbeads were nearly the same. These VPBA containing p HEMA-VPBA microbeads with a swelling ratio of 57 % were used in the adsorption/desorption of human-IgG from aqueous solutions and human plasma. The maximum human-IgG adsorption 139 mg/g polymer was observed at pH 8.5 for HEPES buffer. IgG adsorption from human plasma was 38.75 mg/g. It was also observed that human-IgG could be repeatedly adsorbed and desorbed with p HEMA-VPBA microbeads without significant loss in the adsorption capacity.