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Dergiler / Hacettepe Journal of Biology and Chemistry / 2026 / Volume: 36 Issue: 1

Boronic Acid Functionalized Adsorbents for Antibody Removal

Başak Şaşmaz(Hacettepe University, Department of Chemistry, Biochemistry Division, Ankara)
Gözde Baydemir(Hacettepe University, Department of Chemistry, Biochemistry Division, Ankara)
Nilay Bereli(Hacettepe University, Department of Chemistry, Biochemistry Division, Ankara)
Serap Şenel(Hacettepe University, Department of Chemistry, Biochemistry Division, Ankara)
Handan Yavuz(Hacettepe University, Department of Chemistry, Biochemistry Division, Ankara)
Sayfa
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Yayın Tarihi
2008-01-01
DOI
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It was also observed that human-IgG could be repeatedly adsorbed and desorbed with p HEMA-VPBA microbeads without significant loss in the adsorption capacity.

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Amaç & Hipotez:In this study a novel approach was developed to obtain an efficient separation of human-IgG from human plasma. 4- Vinylphenylboronic acid VPBA and 2-hydroxyethylmethacrylate HEMA were chosen as monomers.
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Temel Bulgular & Veri:Spherical microbeads with an average size of 100-140 µm were obtained by the radical suspension polymerization of VPBA and HEMA conducted in an aqueous dispersion medium.
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Sonuç & Katkı:It was also observed that human-IgG could be repeatedly adsorbed and desorbed with p HEMA-VPBA microbeads without significant loss in the adsorption capacity.
🔬Tür:Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Boronic Acid · Antibody Removal · Boranate affinity

Abstract

In this study a novel approach was developed to obtain an efficient separation of human-IgG from human plasma. 4- Vinylphenylboronic acid VPBA and 2-hydroxyethylmethacrylate HEMA were chosen as monomers. Spherical microbeads with an average size of 100-140 µm were obtained by the radical suspension polymerization of VPBA and HEMA conducted in an aqueous dispersion medium. VPBA monomer was characterized by FTIR. p HEMA-VPBA beads were characterized by swelling studies, FTIR and SEM. The pHEMA and p HEMA-VPBA microbeads were contacted with blood in in-vitro systems. Loss of the blood cells and the clotting times were also followed. Good blood compatibility properties of both pHEMA and p HEMA-VPBA were clearly observed with blood coagulation experiments. Loss of cells in the blood contacting with pHEMA and p HEMA-VPBA microbeads were nearly the same. These VPBA containing p HEMA-VPBA microbeads with a swelling ratio of 57 % were used in the adsorption/desorption of human-IgG from aqueous solutions and human plasma. The maximum human-IgG adsorption 139 mg/g polymer was observed at pH 8.5 for HEPES buffer. IgG adsorption from human plasma was 38.75 mg/g. It was also observed that human-IgG could be repeatedly adsorbed and desorbed with p HEMA-VPBA microbeads without significant loss in the adsorption capacity.

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Bu Makaleye Atıf Yap

Şaşmaz, B., Baydemir, G., Bereli, N., Şenel, S., Yavuz, H. (2008). Boronic Acid Functionalized Adsorbents for Antibody Removal. Hacettepe Journal of Biology and Chemistry, Volume: 36 Issue: 1. https://ajindex.com/articles/1271149

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