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Dergiler / Hacettepe Journal of Biology and Chemistry / 2018 / Cilt: 46 - Sayı: 2

Simultaneous Determination of Three Phenolic Compounds in Water Samples by Pre-column Derivatization Coupled with Reversed-Phase High Performance Liquid Chromatography

Adem ASAN
M.umut KONANÇ
Bedia AKMEŞE
Sayfa
147–157
Yayın Tarihi
2018-06-03
DOI
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The method was applied to the analysis of phenols in different water samples.

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Amaç & Hipotez:I n this study, a sensitive and accurate method for simultaneous separation and determination of three phenolic compounds (phenol, m-cresol and resorcinol) in water by revesed-phase high performance liquid chromatography using uv-visible detection has been described.
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Temel Bulgular & Veri:Pre-column derivatization with 4-aminoantipyrine is used for the seperation and determination phenol, m-cresol and resorcinol in water.
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Sonuç & Katkı:The method was applied to the analysis of phenols in different water samples.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Phenols · derivatization · reversed-phase liquid chromatography

Abstract

I n this study, a sensitive and accurate method for simultaneous separation and determination of three phenolic compounds (phenol, m-cresol and resorcinol) in water by revesed-phase high performance liquid chromatography using uv-visible detection has been described. Pre-column derivatization with 4-aminoantipyrine is used for the seperation and determination phenol, m-cresol and resorcinol in water. The derivatives formed within 5 min were extracted with chloroform and then analyzed by liquid chromatography with UV-visible detection at 440 nm. Chromatographic separation was performed using a reversed-phase column and acetonitrile-water (45:55%, v/v) as the mobile phase. The three derivatives were elueted in 13 min. The detection limits of phenol, m-cresol and resorcinol in a standard water sample were between 0.07 and 0.09 μg.L-1 for 100 mL respectively. The recoveries of the derivatives from pure water were betweeen 97.1-102.3% within relative standard deviations of 2.3-4.7%. The method was applied to the analysis of phenols in different water samples.

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Bu Makaleye Atıf Yap

ASAN, A., KONANÇ, M., AKMEŞE, B. (2018). Simultaneous Determination of Three Phenolic Compounds in Water Samples by Pre-column Derivatization Coupled with Reversed-Phase High Performance Liquid Chromatography. Hacettepe Journal of Biology and Chemistry, Cilt: 46 - Sayı: 2, 147–157. https://ajindex.com/articles/255646

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Kaynakça (12)

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