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Dergiler / Gazi University Journal of Science / 2016 / Cilt: 29 - Sayı: 2

PREPARATION OF A NEW URIC ACID BIOSENSOR WITH IMMOBILIZATION OF URICASE UPON POLYPYRROLE-PARATOLUENE SULPHONATE FILM

Servet ÇETE
Gökçen Karpuz
Ahmet Yaşar
Sayfa
293–301
Yayın Tarihi
2016-06-20
DOI
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The performance of the biosensor was measured in serum of healthy individuals.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:In this study, a new amperometric biosensor based on immobilized uricase was developed for the determination of uric acid.
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Temel Bulgular & Veri:Determination of uric acid was performed by oxidation of enzymically generated H2O2 at 0.3 V.
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Sonuç & Katkı:The performance of the biosensor was measured in serum of healthy individuals.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Uric acid · uricase · biosensor

Abstract

In this study, a new amperometric biosensor based on immobilized uricase was developed for the determination of uric acid. Polypyrrole-p-toluene sulphonate film was prepared on the surface of a platinum (Pt) electrode by electropolymerization of pyrrole in the presence of p-toluene sulphonate as an anionic dopant. Uricase enzyme were immobilized in polypyrrole-p-toluene sulphonate via the entrapment method. Determination of uric acid was performed by oxidation of enzymically generated H2O2 at 0.3 V. Some factors that affect response current were studied such as temperature, pH and substrate concentration. Operation stability and storage stability of the biosensor was determined. The biosensor retained 76.6% of its initial performance after 30 assays and it lost 58% of its initial performance after 60 days. Effects of interferants on the current response of the biosensor were examined. The performance of the biosensor was measured in serum of healthy individuals.

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Bu Makaleye Atıf Yap

ÇETE, S., Karpuz, G., Yaşar, A. (2016). PREPARATION OF A NEW URIC ACID BIOSENSOR WITH IMMOBILIZATION OF URICASE UPON POLYPYRROLE-PARATOLUENE SULPHONATE FILM. Gazi University Journal of Science, Cilt: 29 - Sayı: 2, 293–301. https://ajindex.com/articles/255422

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Kaynakça (18)

  1. Eswara U.S., Dutt H., Mottola A., Anal. Chem., 46:1777, (1974).
  2. Raj C.R., Ohsaka T., J Electroanal. Chem., 540:69, (2003).
  3. Arora K., Tomar M., Gupta V., “Highly sensitive and selective uric acid biosensor based on RF sputtered NiO thin film”, Biosensors and Bioelectronics, 30:333–336, (2011).
  4. Burtic C.A., Ashwood E.R., Teitz Textbook of Clinical Chemistry second ed.WB Saunders, Philadelphia (1994).
  5. Rawal R., Chawla S., Chauhan N., Dahiya T., Pundir C.S., “Construction of amperometric uric acid biosensor based on uricase immobilized on PBNPs/cMWCNT/PANI/Au composite”, International Journal of Biological Macromolecules, 50:112– 118 (2012).
  6. Zhao Y.S., Liao F.H., “Serum uric acid and hyperuricemia associated disease”, Int. J. Intern. Med., 34:41–47 (2007).
  7. Becker M.A., Jolly M., “Hyperuricemia and associated diseases”, Rheum. Dis. Clin. North Ame., 32:275–293 (2006).
  8. Chauhan N., Pundir C.S., “An amperometric uric acid biosensor based on multiwalled carbon nanotube–gold nanoparticle composite”, Analytical Biochemistry, 413:97–103 (2011).
  9. Bhargava A.K., Lal H., Pundir C.S., J Biochem. Biophys. Methods, 39:125–136 (1999).
  10. Zhang F., Wang X., Ai S., Sun Z., Wan Q., Zhu Z., Xian Y., Jin L., Yamamoto K., Wang J., Golden T., Tuzhi P., Anal. Chem., 59:740–744 (1987).
  11. Greenberg M.L., Hershfield M.S., Anal. Biochem., 176:290–293 (1989).
  12. Yao D., Vlessidis A.G., Evmiridis N.P., Anal. Chim. Acta., 478:23–30 (2003).
  13. Galban J., Andreu Y., Almenara M.J., de Marcos S., Castillo J.R., Talanta, 54:847–854 (2001).
  14. Yao D., Vlessidis A.G., Evmiridis N.P., Anal. Chim. Acta., 467:133–144 (2002).
  15. Byfield M.P., Abuknesha R.A., “Biochemical aspects of biosensors”, Biosens. Bioelectron., 9:373–399 (1994).
  16. Chu H., Wei X., Wu M., Yan J., Tu Y., “An electrochemiluminescent biosensor based on polypyrrole immobilized uricase for ultrasensitive uric acid detection”, Sensors and Actuators B, 163:247– 252 (2012).
  17. Clark L.C., Lyones C., “Electrode systems for continuous monitoring in cardiovascular surgery”, Ann. N.Y. Acad. Sci., 102:29–45 (1962). Willner I., “Bioelectronics: biomaterials for sensors, fuel cells, and circuitry”, Science, 298:2407–2408 (2002). Turner A.P.F., “Biochemistry: biosensors-sense and sensitivity”, Science, 90:1315–1317 (2000). Mu S., “Bioelectrochemical response of the poloaniline galactose oxidase electrode” J. Electroanal. Chem., 370 (1/2):135–139 (1994).
  18. Pan X., Zhou S., Chen C., Kan J., “Preparation and properties of an uricase biosensor based on copolymer of o-aminop