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AI InsightEtOAc extract, which showed the highest antimicrobial activity, was further used for isolation.
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Özet
Chromatographic studies (CC, VLC, MPLC, and PTLC) on ethyl acetate extract from the leaves of Quercus aucheri yielded 2 flavonoids (quercetin 3-O-a-L-arabinopyranoside (1), quercetin 3-O-b-D-galactopyranoside (2)) and 2 tannin precursors and a procyanidin [(isolated as peracetates of (+)-catechin (3a), (+)-gallocatechin (4a) and epicatechin-(4b \to 8)-catechin (5a))]. The structures of the compounds were elucidated by UV, 1D-NMR (1H, 13C, TOCSY) and 2D-NMR (COSY, HSQC, HMBC) techniques. Different extracts (80% MeOH, EtOAc, n-BuOH and H2O) from the leaves of Q. aucheri were investigated for their antimicrobial activity against 2 Gram-positive and 2 Gram-negative bacteria and 3 yeast-like fungi by a broth microdilution method. EtOAc extract, which showed the highest antimicrobial activity, was further used for isolation.
Abstract
Chromatographic studies (CC, VLC, MPLC, and PTLC) on ethyl acetate extract from the leaves of Quercus aucheri yielded 2 flavonoids (quercetin 3-O-a-L-arabinopyranoside (1), quercetin 3-O-b-D-galactopyranoside (2)) and 2 tannin precursors and a procyanidin [(isolated as peracetates of (+)-catechin (3a), (+)-gallocatechin (4a) and epicatechin-(4b \to 8)-catechin (5a))]. The structures of the compounds were elucidated by UV, 1D-NMR (1H, 13C, TOCSY) and 2D-NMR (COSY, HSQC, HMBC) techniques. Different extracts (80% MeOH, EtOAc, n-BuOH and H2O) from the leaves of Q. aucheri were investigated for their antimicrobial activity against 2 Gram-positive and 2 Gram-negative bacteria and 3 yeast-like fungi by a broth microdilution method. EtOAc extract, which showed the highest antimicrobial activity, was further used for isolation.