KaydedilenlerDerginizi ekleyin

Dergiler / Communications Faculty of Sciences University of Ankara Series C Biology / 2021 / Cilt: 30 - Sayı: 1

CHARACTERIZATION OF ITS1 SECONDARY STRUCTURE IN TEN SPECIES OF COLUTEOCARPEAE (BRASSICACEAE) AND ITS TAXONOMICAL UTILITY

Kurtuluş ÖZGİŞİ
Sayfa
1–12
Yayın Tarihi
2021-06-30
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Since previous studies reveals other species, that belong different tribes or lineages of Brassicaceae show similar ITS1 secondary structure, it is not a useful delimitation tool for investigated species in terms of taxonomy.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Öz Utility of the internal transcribed spacers (ITSs) of ribosomal RNA sequences to infer phylogenetic relationships among organisms have been proven.
📊
Temel Bulgular & Veri:Although ITS1 and ITS2 are highly variable in sequence, they have conserved structures that have a key function in the processing of rRNA gene transcripts.
💡
Sonuç & Katkı:Since previous studies reveals other species, that belong different tribes or lineages of Brassicaceae show similar ITS1 secondary structure, it is not a useful delimitation tool for investigated species in terms of taxonomy.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Anahtar Kelimeler Coluteocarpeae · Brassicaceae · secondary structure

Özet

Öz Utility of the internal transcribed spacers (ITSs) of ribosomal RNA sequences to infer phylogenetic relationships among organisms have been proven. Although ITS1 and ITS2 are highly variable in sequence, they have conserved structures that have a key function in the processing of rRNA gene transcripts. Determining of such a conserved motif can help to identify relationships between organisms. Since ITS2 has much more conserved secondary structure, structural properties of ITS1 are generally neglected by researchers. In this study, ITS1 secondary structures of ten representative species, which were once assigned under different genera, of tribe Coluteocarpeae were determined. Also taxonomical utility of ITS1 secondary structure was also tested. Analyses indicate that there are four different types (4-, 6-, 7- and 8 hairpin) of secondary structures. On the other hand, each sequences have a conserved region that is common among land plants. Since previous studies reveals other species, that belong different tribes or lineages of Brassicaceae show similar ITS1 secondary structure, it is not a useful delimitation tool for investigated species in terms of taxonomy.

🔖

Bu Makaleye Atıf Yap

ÖZGİŞİ, K. (2021). CHARACTERIZATION OF ITS1 SECONDARY STRUCTURE IN TEN SPECIES OF COLUTEOCARPEAE (BRASSICACEAE) AND ITS TAXONOMICAL UTILITY. Communications Faculty of Sciences University of Ankara Series C Biology, Cilt: 30 - Sayı: 1, 1–12. https://ajindex.com/articles/247848

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (11)

  1. Hillis, D.M., Dixon M. T., Ribosomal DNA: molecular evolution and phylogenetic inference, The Quarterly review of biology, 66 (4) (1991), 411-453.
  2. Warwick, S.I., Mummenhoff, K., Sauder, C.A., Koch, M.A., Al-Shehbaz, I.A., Closing the gaps: phylogenetic relationships in the Brassicaceae based on DNA sequence data of nuclear ribosomal ITS region, Plant Systematics and Evolution, 285 (3-4) (2010), 209-232. https://doi.org/10.1007/s00606-010-0271-8.
  3. Baldwin, B. G., Sanderson, M. J., Porter, J. M., Wojciechowski, M. F., Campbell, C. S., Donoghue, M. J., The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny, Annals of the Missouri Botanical Garden, (1995), 247-277.
  4. Musters, W., Boon, K., Van der Sande, C. A., van Heerikhuizen, H., & Planta, R. J., Functional analysis of transcribed spacers of yeast ribosomal DNA, The EMBO journal, 9 (12) (1990), 3989-3996. https://doi.org/10.1002/j.1460-2075.1990.tb07620.x
  5. Mai, J. C., Coleman, A. W., The internal transcribed spacer 2 exhibits a common secondary structure in green algae and flowering plants, Journal of Molecular Evolution, 44 (3) (1997), 258-271.
  6. Coleman, A. W., Is there a molecular key to the level of “biological species” in eukaryotes? A DNA guide, Molecular phylogenetics and evolution, 50(1) (2009), 197-203. https://doi.org/10.1016/j.ympev.2008.10.008.
  7. Wolf, M., Chen, S., Song, J., Ankenbrand, M., Müller, T., Compensatory base changes in ITS2 secondary structures correlate with the biological species concept despite intragenomic variability in ITS2 sequences–a proof of concept, PloS one,8(6) (2013), e66726. https://doi.org/10.1371/journal.pone.0066726.
  8. Coleman, A. W., Vacquier, V. D., Exploring the phylogenetic utility of ITS sequences for animals: a test case for abalone (Haliotis), Journal of molecular evolution, 54(2) (2002), 246-257. https://doi.org/ 10.1007/s00239-001-0006-0.
  9. Mullineux, T., Hausner, G., Evolution of rDNA ITS1 and ITS2 sequences and RNA secondary structures within members of the fungal genera Grosmannia and Leptographium, Fungal Genetics and Biology, 46(11) (2009), 855-867. https://doi.org/10.1016/j.fgb.2009.08.001.
  10. Saha, P. S., Sengupta, M., Jha, S., Ribosomal DNA ITS1, 5.8 S and ITS2 secondary structure, nuclear DNA content and phytochemical analyses reveal distinctive characteristics of four subclades of Protasparagus, Journal of Systematics and Evolution, 55(1) (2017), 54-70. https://doi.org/10.1111/jse.12221.
  11. Karpenko, N., Martyniuk, V., Tyshch