KaydedilenlerDerginizi ekleyin

Dergiler / Recent Trends in Pharmacology / 2024 / Volume: 2 Issue: 3

Investigation of the Neurotoxic Effects of Dimethyl Phthalate and Diisobutyl Phthalate on SH-SY5Y Neuroblastoma Cells

Mehtap Kara(ISTANBUL UNIVERSITY)0000-0001-7764-5593
Zeynep Göker(ISTANBUL UNIVERSITY)0009-0004-1597-0015
Ayşenur Erdinç(ISTANBUL UNIVERSITY)0009-0006-3288-720X
Erkan Gülgen(ISTANBUL UNIVERSITY)0009-0003-2253-6420
Yağmur Emre Arıcan(SULEYMAN DEMIREL UNIVERSITY)0000-0002-6910-2553
Çiğdem Sevim(KASTAMONU UNIVERSITY)0000-0002-0575-3090
Sayfa
—
Yayın Tarihi
2024-12-30
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

This study highlights the compounded risk of phthalate mixtures, urging comprehensive risk assessments and regulatory policies to mitigate human health risks from combined chemical exposures.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Endocrine disruptors, particularly phthalates like Dimethyl phthalate (DMP) and Diisobutyl phthalate (DiBP), are prevalent environmental contaminants posing significant health risks.
📊
Temel Bulgular & Veri:This study investigates the combined neurotoxic effects of DMP and DiBP on SH-SY5Y neuroblastoma cells by analyzing cytotoxicity, oxidative stress, and apoptosis.
💡
Sonuç & Katkı:This study highlights the compounded risk of phthalate mixtures, urging comprehensive risk assessments and regulatory policies to mitigate human health risks from combined chemical exposures.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:apoptosis · endocrine disruptors · neurotoxicity

Abstract

Endocrine disruptors, particularly phthalates like Dimethyl phthalate (DMP) and Diisobutyl phthalate (DiBP), are prevalent environmental contaminants posing significant health risks. This study investigates the combined neurotoxic effects of DMP and DiBP on SH-SY5Y neuroblastoma cells by analyzing cytotoxicity, oxidative stress, and apoptosis. Using MTT and Neutral Red Uptake assays, we determined the IC50 values for DMP and DiBP as 11.35 mM and 1.307 mM, respectively. Flow cytometry revealed increased Reactive Oxygen Species (ROS) levels, indicating oxidative stress, while apoptosis assays showed enhanced cell death with combined phthalate exposure. The results demonstrate a synergistic effect, exacerbating cytotoxic and oxidative damage beyond individual exposures. This study highlights the compounded risk of phthalate mixtures, urging comprehensive risk assessments and regulatory policies to mitigate human health risks from combined chemical exposures.

🔖

Bu Makaleye Atıf Yap

Kara, M., Göker, Z., Erdinç, A., Gülgen, E., Arıcan, Y. E., Sevim, Ç. (2024). Investigation of the Neurotoxic Effects of Dimethyl Phthalate and Diisobutyl Phthalate on SH-SY5Y Neuroblastoma Cells. Recent Trends in Pharmacology, Volume: 2 Issue: 3. https://doi.org/10.62425/rtpharma.1530821

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (25)

  1. Chen, X., Xu, S., Tan, T., Lee, S. T., Cheng, S. H., Lee, F. W. F., Xu, S. J. L., & Ho, K. C. (2014). Toxicity and estrogenic endocrine disrupting activity of phthalates and their mixtures. International Journal of Environmental Research and Public Health, 11(3), 3156–3168. https://doi.org/10.3390/IJERPH110303156
  2. Cong, B., Liu, C., Wang, L., & Chai, Y. (2020). The impact on antioxidant enzyme activity and related gene expression following adult zebrafish (Danio rerio) exposure to dimethyl phthalate. Animals, 10(4), 717. https://doi.org/10.3390/ANI10040717
  3. Hlisníková, H., Petrovičová, I., Kolena, B., Šidlovská, M., & Sirotkin, A. (2021). Effects and mechanisms of phthalates’ action on neurological processes and neural health: A literature review. Pharmacological Reports, 73(2), 386–404. https://doi.org/10.1007/S43440-021-00215-5
  4. Kara, M., Boran, T., Öztaş, E., Jannuzzi, A. T., Özden, S., & Özhan, G. (2022). Zoledronic acid-induced oxidative damage and endoplasmic reticulum stress-mediated apoptosis in human embryonic kidney (HEK-293) cells. Journal of Biochemical and Molecular Toxicology, 36(8), e23083. https://doi.org/10.1002/JBT.23083
  5. Kleinsasser, N. H., Wallner, B. C., Kastenbauer, E. R., Weissacher, H., & Harréus, U. A. (2001). Genotoxicity of di-butyl-phthalate and di-iso-butyl-phthalate in human lymphocytes and mucosal cells. Teratogenesis, Carcinogenesis, and Mutagenesis, 21(3), 189–196. https://doi.org/10.1002/tcm.1007
  6. Kleinsasser, N. H., Weissacher, H., Kastenbauer, E. R., Dirschedl, P., Wallner, B. C., & Harréus, U. A. (2000). Altered genotoxicity in mucosal cells of head and neck cancer patients due to environmental pollutants. European Archives of Oto-Rhino-Laryngology, 257(6), 337–342. https://doi.org/10.1007/S004059900220
  7. Mahmoud, A., Ezgi, Ö., Merve, A., & Özhan, G. (2016). In vitro toxicological assessment of magnesium oxide nanoparticle exposure in several mammalian cell types. International Journal of Toxicology, 35(4), 429–437. https://doi.org/10.1177/1091581816648624
  8. Meeker, J. D. (2012). Exposure to environmental endocrine disruptors and child development. Archives of Pediatrics & Adolescent Medicine, 166(10), 952–958. https://doi.org/10.1001/ARCHPEDIATRICS.2012.241
  9. Nahla, E., Arya, P., Maneesha, P., & Chitra, K. C. (2024). Exposure to the plasticizer dibutyl phthalate causes oxidative stress and neurotoxicity in brain tissue. Environmental Science and Pollution Research, 31(14), 21399–21414. https://doi.org/10.1007/S11356-024-32604-7
  10. Rodrigues, R. M., Stinckens, M., Ates, G., & Vanhaecke, T. (2023). Neutral red uptake assay to assess cytotoxicity in vitro. Methods in Molecular Biology, 2644, 237–245. https://doi.org/10.1007/978-1-0716-3052-5_15
  11. Sellinger, E., Riesgo, V., Brinks, A. S., & Willing, J. (2021). Perinatal phthalate exposure increases developmental apoptosis in the rat medial prefrontal cortex. Neurotoxicology, 87, 167–173. https://doi.org/10.1016/j.neuro.2021.09.007
  12. Sevim, C., Taghizadehghalehjoughi, A., Kara, M., Nosyrev, A. E., Nițulescu, G. M., Margină, D., & Tsatsakis, A. (2024). Investigation of the effects of metformin on the miR-21/PTEN/AKT pathway in HT-29 human colorectal adenocarcinoma cell and HUVEC co-culture. Farmacia, 72(1). https://doi.org/10.31925/farmacia.2024.1.5
  13. Tran, C. M., Do, T. N., & Kim, K. T. (2021). Comparative analysis of neurotoxicity of six phthalates in zebrafish embryos. Toxics, 9(1), 1–11. https://doi.org/10.3390/TOXICS9010005
  14. Wang, G., Shen, J., Lin, Y., Zhai, L., Guan, Q., & Shen, H. (2024). Dimethyl phthalate exposure induces cognitive impairment through COX2-mediated microglial activation. Research Square. https://doi.org/10.21203/RS.3.RS-4081530/V1
  15. Wang, Y., & Qian, H. (2021). Phthalates and their impacts on human health. Healthcare, 9(5), 603. https://doi.org/10.3390/HEALTHCARE9050603
  16. Yost, E. E., Euling, S. Y., Weaver, J. A., Beverly, B. E. J., Keshava, N., Mudipalli, A., Arzuaga, X., Blessinger, T., Dishaw, L., Hotchkiss, A., & Makris, S. L. (2019). Hazards of diisobutyl phthalate (DIBP) exposure: A systematic review of animal toxicology studies. Environment International, 125, 579–594. https://doi.org/10.1016/J.ENVINT.2018.09.038
  17. Chi, Z., Lin, H., Wang, X., Meng, X., Zhou, J., Xiang, L., Cao, G., Wu, P., Cai, Z., & Zhao, X. (2022). Dimethyl phthalate induces blood immunotoxicity through oxidative damage and caspase-dependent apoptosis. Science of The Total Environment, 838, 156047. https://doi.org/10.1016/J.SCITOTENV.2022.156047
  18. Kara, M., Oztas, E., Ramazanoğulları, R., Kouretas, D., Nepka, C., Tsatsakis, A. M., & Veskoukis, A. S. (2020). Benomyl, a benzimidazole fungicide, induces oxidative stress and apoptosis in neural cells. Toxicology Reports, 7, 501–509. https://doi.org/10.1016/J.TOXREP.2020.04.001
  19. Mahmoud, A., Öztaş, E., Merve, A., & Özhan, G. (2016). In vitro toxicological assessment of magnesium oxide nanoparticle exposure in several mammalian cell types. International Journal of Toxicology, 35(4), 429–437. https://doi.org/10.1177/1091581816648624
  20. Rodrigues, R. M., Stinckens, M., Ates, G., & Vanhaecke, T. (2023). Neutral red uptake assay to assess cytotoxicity in vitro. Methods in Molecular Biology (Clifton, N.J.), 2644, 237–245. https://doi.org/10.1007/978-1-0716-3052-5_15
  21. Sellinger, E., Riesgo, V., & Brinks, A. (2021). Perinatal phthalate exposure increases developmental apoptosis in the rat medial prefrontal cortex. Neurotoxicology. https://doi.org/10.1016/j.neuro.2021.03.010
  22. Sevim, C., Taghizadehghalehjoughi, A., Kara, M., Nosyrev, A. E., Nițulescu, G. M., Margină, D., & Tsatsakis, A. (2024). Investigation of the effects of metformin on the miR-21/PTEN/Akt pathway in HT-29 human colorectal adenocarcinoma cell line. Farmacia, 72(1). https://doi.org/10.31925/farmacia.2024.1.5
  23. Wang, G., Shen, J., Lin, Y., Zhai, L., Guan, Q., & Shen, H. (2024a). Dimethyl phthalate exposure induces cognitive impairment through COX2-mediated microglial activation. Research Square. https://doi.org/10.21203/RS.3.RS-4081530/V1
  24. Wang, G., Shen, J., Lin, Y., Zhai, L., Guan, Q., & Shen, H. (2024b). Dimethyl phthalate exposure induces cognitive impairment through COX2-mediated microglial activation. Research Square. https://doi.org/10.21203/RS.3.RS-4081530/V1
  25. Zhang, Y., Lyu, L., Tao, Y., Ju, H., & Chen, J. (2022). Health risks of phthalates: A review of immunotoxicity. Environmental Pollution, 313, 120173. https://doi.org/10.1016/J.ENVPOL.2022.120173