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Dergiler / Journal of Agricultural Biotechnology / 2026 / Cilt: 7 Sayı: 1

Effects of Seed Priming Treatments on Germination and Seedling Growth of Common Vetch (Vicia sativa L.) Under Arsenic Stress

Arsenik Stresi Altındaki Adi Fiğde (Vicia sativa L.) Priming Uygulamalarının Çimlenme ve Fide Büyümesi Üzerine Etkileri

Mehmet Öten(Sakarya Uygulamalı Bilimler Üniversitesi)0000-0002-3737-2356
Baha Ölmez0009-0007-9245-4868
Sayfa
23–31
Yayın Tarihi
2026-07-03
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

In conclusion, it was determined that GA3 seed priming is an effective and practical agronomic strategy for alleviating arsenic heavy metal phytotoxicity and sustaining biomass stability in common vetch seedlings.

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Amaç & Hipotez:In this study, the inhibitory effects of increasing arsenic concentrations (0, 1, 2, 3 mg L-1) on the germination and early seedling development of common vetch (Vicia sativa L. cv. "Gülhan") seeds were determined.
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Temel Bulgular & Veri:Furthermore, the potentials of hydropriming and gibberellic acid (GA3 5 mg L-1) seed priming treatments to mitigate heavy metal toxicity and preserve plant growth parameters were comparatively evaluated.
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Sonuç & Katkı:In conclusion, it was determined that GA3 seed priming is an effective and practical agronomic strategy for alleviating arsenic heavy metal phytotoxicity and sustaining biomass stability in common vetch seedlings.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Heavy metal · Biomass · Gibberellic acid

Özet

In this study, the inhibitory effects of increasing arsenic concentrations (0, 1, 2, 3 mg L-1) on the germination and early seedling development of common vetch (Vicia sativa L. cv. "Gülhan") seeds were determined. Furthermore, the potentials of hydropriming and gibberellic acid (GA3 5 mg L-1) seed priming treatments to mitigate heavy metal toxicity and preserve plant growth parameters were comparatively evaluated. The petri experiment was conducted in a factorial arrangement based on a Completely Randomized Design, where germination characteristics, seedling morphology, and biomass accumulation traits were assessed at the end of a 14-day incubation period. The experimental results revealed that the Pre-treatment x Arsenic Dose interaction had significant (p < 0.01) effect on all evaluated morphological and physiological parameters. Arsenic stress, in the unprimed hydropriming group, suppressed the final germination rate to 84.4%, while drastically reducing root length (2.20 cm at 2 mg L-1 arsenic dose) and net dry weight (0.28 g at 3 mg L-1 arsenic dose). Conversely, GA3 priming successfully maintained the final germination rate at 94.8% even under the highest arsenic dose (3 mg L-1), exhibiting a protective effect by preserving root length (4.40 cm) and total seedling length (8.90 cm). Within the scope of Principal Component and Cluster Analyses (PCA and HCA), the Ward dendrogram and biplot presentations confirmed the segregation of the dataset into two distinct homeostatic clusters designated as "Metabolic Stability" and "Physiological Collapse." Multivariate analyses further demonstrated that GA3-primed seedlings grouped within the same tolerance cluster as the non-stressed control plants despite the presence of arsenic, whereas the germination speed index operated via an independent physiological dynamic separate from subsequent seedling growth stability. In conclusion, it was determined that GA3 seed priming is an effective and practical agronomic strategy for alleviating arsenic heavy metal phytotoxicity and sustaining biomass stability in common vetch seedlings.

Abstract

Bu araştırmada, adi fiğ (Vicia sativa L., Gülhan çeşidi) tohumlarında, artan arsenik konsantrasyonlarının (0, 1, 2, 3 mg L-1) çimlenme ve erken fide gelişimi üzerindeki baskılayıcı etkileri belirlenmiştir. Ayrıca, saf su (hidropriming) ve giberellik asit (GA3 5 mg L-1) ön uygulamalarının, ağır metal toksisitesini hafifletme ve bitkisel büyüme parametrelerini koruma potansiyelleri karşılaştırmalı olarak incelenmiştir. Tesadüf Parselleri Deneme Deseninde faktöriyel düzene göre yürütülen petri denemesinde, 14. gün sonunda tohumların çimlenme ve ki fide morfolojisi ile biyokütle üretim özellikleri değerlendirilmiştir. Araştırma sonuçları, Ön Uygulama x Arsenik Dozu interaksiyonunun incelenen tüm morfolojik ve fizyolojik parametreler üzerinde anlamlı (p < 0.01) etkiye sahip olduğunu göstermiştir. Arsenik stresi, hormonal destek almayan saf su grubunda nihai çimlenme oranını %84.4’e geriletmiş, kök uzunluğunu (2 mg L-1 arsenik dozunda 2.20 cm) ve net kuru ağırlığı (3 mg L-1 arsenik dozunda 0.28 g) dramatik şekilde düşürmüştür. Buna karşın GA3 priming uygulaması, en yüksek arsenik dozunda (3 mg L-1) nihai çimlenmeyi %94.8 seviyesinde tutmayı başarmış; kök boyunu (4.40 cm) ve fide toplam uzunluğunu (8.90 cm) koruyarak koruyucu bir etki sergilemiştir. Temel Bileşen ve Kümeleme Analizleri (PCA ve HCA) kapsamında üretilen Ward dendrogramı ve biplot grafikleri, veri setinin "Metabolik Kararlılık" ile "Fizyolojik Çöküş" olarak iki ana homeostatik kümeye ayrıldığını doğrulamıştır. Çok değişkenli analizler, GA3 uygulanan fidelerin arsenik varlığında bile stressiz kontrol bitkileriyle aynı tolerans kümesinde yer aldığını, ancak çimlenme hızı indeksinin sonraki fide büyüme kararlılığından bağımsız bir fizyolojik dinamikle hareket ettiğini ortaya koymuştur. Sonuç olarak, GA3 tohum priming işleminin, adi fiğ fidelerinde arsenik ağır metal fitotoksisitesini hafifletmede ve biyokütle kararlılığını sürdürmede etkili ve pratik bir agronomik strateji olduğu saptanmıştır.

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Bu Makaleye Atıf Yap

Öten, M., Ölmez, B. (2026). Effects of Seed Priming Treatments on Germination and Seedling Growth of Common Vetch (Vicia sativa L.) Under Arsenic Stress. Journal of Agricultural Biotechnology, Cilt: 7 Sayı: 1, 23–31. https://doi.org/10.58728/joinabt.1970528

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