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Dergiler / Turkish Journal of Veterinary Research / 2026 / Volume: 9 Issue: 1

The efficacy of human recombinant luteinizing hormone for in vitro embryo production in sheep

Ramazan Arıcı(ISTANBUL UNIVERSITY-CERRAHPASA, FACULTY OF VETERINARY MEDICINE)0000-0002-2236-2526
Kamber Demir(ISTANBUL UNIVERSITY-CERRAHPASA, FACULTY OF VETERINARY MEDICINE, VETERINARY MEDICINE PR.)0000-0002-4561-6189
Selin Yağcioğlu(ISTANBUL UNIVERSITY-CERRAHPASA, FACULTY OF VETERINARY MEDICINE)0000-0002-9355-498X
Gül Bakirer Ozturk(ISTANBUL UNIVERSITY)0000-0003-0185-8239
Ahmet Eser(SIIRT UNIVERSITY)0000-0003-1326-2678
Nur Ersoy(ISTANBUL UNIVERSITY-CERRAHPASA, FACULTY OF VETERINARY MEDICINE)0000-0002-7460-0450
Israa Faris(ISTANBUL UNIVERSITY-CERRAHPASA, FACULTY OF VETERINARY MEDICINE)0000-0002-4890-9892
Andaç Kılıçkap(ISTANBUL MEDENIYET UNIVERSITY)0000-0002-4479-3191
Mithat Evecen(ISTANBUL UNIVERSITY-CERRAHPASA, FACULTY OF VETERINARY MEDICINE)0000-0002-0219-6997
Sema Birler(ISTANBUL UNIVERSITY-CERRAHPASA, FACULTY OF VETERINARY MEDICINE)0000-0001-5069-5427
Serhat Pabuccuoğlu(ISTANBUL UNIVERSITY-CERRAHPASA, FACULTY OF VETERINARY MEDICINE)0000-0002-6200-3018
Sayfa
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Yayın Tarihi
2025-03-28
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Conclusion: It was concluded that r-LH could be used as an alternative LH source instead of hypophyseal LH.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objective: This study was carried out to assess the ability of recombinant human luteinizing hormone (r-LH) to be used instead of sheep hypophyseal luteinizing hormone (h-LH) in the maturation of sheep oocytes and its influence on embryonic development and quality.
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Temel Bulgular & Veri:For maturation, oocytes (grade 1) were incubated at 38.5°C, 5% CO2 for 24 hours. 0.1 IU/ml LH (from sheep Piturity LH, Sigma®) was added to the culture media of the hypophyseal LH (h -LH) group and 0.1 IU/ml LH (Luveris® 75 IU, Serono) to the Recombinant LH (r-LH) group.
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Sonuç & Katkı:Conclusion: It was concluded that r-LH could be used as an alternative LH source instead of hypophyseal LH.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Embryo development · hypophyseal LH · in vitro maturation

Abstract

Objective: This study was carried out to assess the ability of recombinant human luteinizing hormone (r-LH) to be used instead of sheep hypophyseal luteinizing hormone (h-LH) in the maturation of sheep oocytes and its influence on embryonic development and quality. Materials and Methods: The oocytes were obtained from slaughtered sheep ovaries. For maturation, oocytes (grade 1) were incubated at 38.5°C, 5% CO2 for 24 hours. 0.1 IU/ml LH (from sheep Piturity LH, Sigma®) was added to the culture media of the hypophyseal LH (h -LH) group and 0.1 IU/ml LH (Luveris® 75 IU, Serono) to the Recombinant LH (r-LH) group. In vitro fertilization (20h) and embryo culture were performed at 5% CO2, 5% O2 and 38.5°C incubation conditions. The maturation rates were reported based on the MII stage chromosomal formation and the existence of polar body by bisBenzimide (Hoechst 33342). Embryonic developments were controlled at 3rd and 8th day of in vitro culture. For the embryonic cell count and determination of inner cell mass (ICM) and trophectoderm cell (TC), the differential staining technique was used with Hoechst 33342 + Propidium Iodide (PI). Results: The proportion of cleavage (%), the rate of embryos developing the morula (%) and blastocyst stage (%), and the ICM, TC, and total cell numbers of the embryos were found to be statistically similar in the h-LH and r-LH groups. Conclusion: It was concluded that r-LH could be used as an alternative LH source instead of hypophyseal LH.

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Bu Makaleye Atıf Yap

Arıcı, R., Demir, K., Yağcioğlu, S., Ozturk, G. B., Eser, A., Ersoy, N., Faris, I., Kılıçkap, A., Evecen, M., Birler, S., Pabuccuoğlu, S. (2025). The efficacy of human recombinant luteinizing hormone for in vitro embryo production in sheep. Turkish Journal of Veterinary Research, Volume: 9 Issue: 1. https://doi.org/10.47748/tjvr.1567856

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