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Dergiler / Journal of Istanbul Veterinary Sciences / 2025 / Cilt: 9 Sayı: 3

Determination of the effects of trehalose and iodixanol on kinematic values ​​in frozen ram sperm after thermal stress test

Determination of the effects of trehalose and iodixanol on kinematic values in frozen ram sperm after thermal stress test

M. Ferit Özmen(DICLE UNIVERSITY)0000-0002-5531-220X
Ramazan Arıcı(İSTANBUL ÜNİVERSİTESİ-CERRAHPAŞA)0000-0002-2236-2526
Sayfa
164–168
Yayın Tarihi
2025-12-31
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

However, more comprehensive studies are needed to clearly determine these effects.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Artificial insemination is the most practical and economical method for breeding in domestic animals.
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Temel Bulgular & Veri:The group with the highest VSL value was OP5, and the difference between this group and the TR50/OP2.5 and TR50/OP5 groups was found to be significant.
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Sonuç & Katkı:However, more comprehensive studies are needed to clearly determine these effects.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:tst · ram semen · trehalose

Özet

Artificial insemination is the most practical and economical method for breeding in domestic animals. However, certain physiological characteristics of sheep and the adverse effects that occur after thawing frozen ram semen hinder its widespread use. In the presented study, the effects of extenders created using different combinations of iodixanol and trehalose, in addition to a Tris-based extender, on frozen ram semen were investigated. For this purpose, thermal stress tests were performed using frozen and thawed ram semen, and their effects on spermatozoa kinematic values ​​were determined using computerized analysis devices. VAP, VCL, and ALH values ​​were found to be similar in all groups. The group with the highest VSL value was OP5, and the difference between this group and the TR50/OP2.5 and TR50/OP5 groups was found to be significant. The group with the highest BCF values ​​was the control group, and the differences between this group and the TR50/OP 1.25, TR50/OP 2.5, TR50/OP 5, and OP5/TR25 groups were found to be significant. The highest STR value was found in the TR25 group, and the differences between this group and the TR50/OP 2.5 and TR50/OP 5 groups were found to be significant. The highest LIN value was obtained from the TR50/OP 1.25 group, and the differences between this group and the TR50/OP 2.5 and TR50/OP 5 groups were found to be significant. In conclusion, changes in LIN, VSL, and STR values, which may affect sperm motility, indicate that iodixanol and trehalose, when used in appropriate proportions, have the potential to positively impact spermatological data. However, more comprehensive studies are needed to clearly determine these effects.

Abstract

Artificial insemination is the most practical and economical method for breeding in domestic animals. However, certain physiological characteristics of sheep and the adverse effects that occur after thawing frozen ram semen hinder its widespread use. In the presented study, the effects of extenders created using different combinations of iodixanol and trehalose, in addition to a Tris-based extender, on frozen ram semen were investigated. For this purpose, thermal stress tests were performed using frozen and thawed ram semen, and their effects on spermatozoa kinematic values ​​were determined using computerized analysis devices. VAP, VCL, and ALH values ​​were found to be similar in all groups. The group with the highest VSL value was OP5, and the difference between this group and the TR50/OP2.5 and TR50/OP5 groups was found to be significant. The group with the highest BCF values ​​was the control group, and the differences between this group and the TR50/OP 1.25, TR50/OP 2.5, TR50/OP 5, and OP5/TR25 groups were found to be significant. The highest STR value was found in the TR25 group, and the differences between this group and the TR50/OP 2.5 and TR50/OP 5 groups were found to be significant. The highest LIN value was obtained from the TR50/OP 1.25 group, and the differences between this group and the TR50/OP 2.5 and TR50/OP 5 groups were found to be significant. In conclusion, changes in LIN, VSL, and STR values, which may affect sperm motility, indicate that iodixanol and trehalose, when used in appropriate proportions, have the potential to positively impact spermatological data. However, more comprehensive studies are needed to clearly determine these effects.

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Bu Makaleye Atıf Yap

Özmen, M. F., Arıcı, R. (2025). Determination of the effects of trehalose and iodixanol on kinematic values ​​in frozen ram sperm after thermal stress test. Journal of Istanbul Veterinary Sciences, Cilt: 9 Sayı: 3, 164–168. https://doi.org/10.30704/http-www-jivs-net.1805042

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