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Dergiler / Turkish Journal of Analytical Chemistry / 2025 / Cilt: 7 Sayı: 3

Morus nigra L. Yaprağından Elde Edilen Fitojenik Gümüş ve Bakır Nanopartikülleri Çok İşlevli Ajanlar Olarak: Antioksidan, Antidiyabetik, Antimikrobiyal ve Antikanser Etkisi

Phytogenic Silver and Copper Nanoparticles from Morus nigra L. Leaf as Multifunctional Agents: Antioxidant, Antidiabetic, Antimicrobial, and Anticancer Potency

Osman Akmeşe(GUMUSHANE UNIVERSITY, CENTRAL RESEARCH LABORATORY APPLICATION AND RESEARCH CENTER)0000-0001-9300-8324
Cemalettin Baltacı(Gümüşhane Üniversitesi Mühendislik ve Doğa Bilimleri Fakültesi Gıda Mühendisliği Bölümü)0000-0002-4336-4002
Enes Gültekin(INONU UNIVERSITY, FACULTY OF ARTS AND SCIENCES, DEPARTMENT OF MOLECULAR BIOLOGY AND GENETICS, MOLECULAR BIOLOGY AND GENETICS PR. (ENGLISH))0000-0002-8545-1868
Irmak Icen Taskın(INONU UNIVERSITY, FACULTY OF ARTS AND SCIENCES, DEPARTMENT OF MOLECULAR BIOLOGY AND GENETICS, MOLECULAR BIOLOGY AND GENETICS PR. (ENGLISH))0000-0002-1612-0563
Meryem Rüveyda Sever(INONU UNIVERSITY, FACULTY OF ARTS AND SCIENCES, DEPARTMENT OF MOLECULAR BIOLOGY AND GENETICS, MOLECULAR BIOLOGY AND GENETICS PR. (ENGLISH))0000-0001-9271-1528
Dilek Çam Derin(INONU UNIVERSITY, FACULTY OF ARTS AND SCIENCES, DEPARTMENT OF MOLECULAR BIOLOGY AND GENETICS, MOLECULAR BIOLOGY AND GENETICS PR. (ENGLISH))0000-0001-5617-4067
Ömer Karpuz(GUMUSHANE UNIVERSITY, FACULTY OF ENGINEERING AND NATURAL SCIENCES, DEPARTMENT OF GENETICS AND BIOENGINEERING, GENETICS AND BIOENGINEERING PR.)0000-0002-0546-9831
Sayfa
321–337
Yayın Tarihi
2025-09-30
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Genel olarak, çalışma yeşil sentezlenen nanopartiküllerin çeşitli terapötik uygulamalarını vurgular ve kanser tedavisinde ve antimikrobiyal ilaçlar için güçlendirici olarak potansiyel kullanımlarını önerir.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Çevre dostu ve çok işlevli nanomalzemelerin artan arayışıyla yönlendirilen bu çalışma, alkali sulu koşullar altında Morus nigra yaprak özütü kullanılarak sentezlenen gümüş (YAgNP'ler) ve bakır nanopartiküllerinin (YCuNP'ler) çevre bilincine sahip üretimini, ayrıntılı analizini ve işbirlikçi antibakteriyel etkinliğini araştırmaktadır.
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Temel Bulgular & Veri:Biyojenik yöntemlerle üretilen nanopartiküller, FTIR, XRD, TEM ve EDX enstrümantasyonu ile doğrulandığı gibi, bitki kaynaklı bileşiklerin kolaylaştırdığı etkili indirgeme ve stabilizasyonu öneren benzersiz fiziksel ve kimyasal özellikler göstermiştir.
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Sonuç & Katkı:Genel olarak, çalışma yeşil sentezlenen nanopartiküllerin çeşitli terapötik uygulamalarını vurgular ve kanser tedavisinde ve antimikrobiyal ilaçlar için güçlendirici olarak potansiyel kullanımlarını önerir.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Morus nigra L · Yeşil sentez · Gümüş nanopartiküller

Özet

Çevre dostu ve çok işlevli nanomalzemelerin artan arayışıyla yönlendirilen bu çalışma, alkali sulu koşullar altında Morus nigra yaprak özütü kullanılarak sentezlenen gümüş (YAgNP'ler) ve bakır nanopartiküllerinin (YCuNP'ler) çevre bilincine sahip üretimini, ayrıntılı analizini ve işbirlikçi antibakteriyel etkinliğini araştırmaktadır. Biyojenik yöntemlerle üretilen nanopartiküller, FTIR, XRD, TEM ve EDX enstrümantasyonu ile doğrulandığı gibi, bitki kaynaklı bileşiklerin kolaylaştırdığı etkili indirgeme ve stabilizasyonu öneren benzersiz fiziksel ve kimyasal özellikler göstermiştir. Bu çalışmada sentezlenen YAgNP'ler ve YCuNP'ler, diyabet yönetimindeki olası rollerini vurgulayarak belirgin şekilde geliştirilmiş antioksidan kapasiteleri ve önemli α-amilaz ve α-glukozidaz inhibisyonu göstermiştir. Antimikrobiyal analizler, çözelti fazı analizlerinde YAgNP'lerin üstün aktivitesini gösterirken, YCuNP'ler katı difüzyon tabanlı kurulumlarda daha güçlü inhibisyon göstermiştir. Özellikle, E. coli ve S. aureus dahil olmak üzere birden fazla Gram-pozitif ve Gram-negatif suşta YAgNP'ler için daha düşük MIC ve MBC konsantrasyonları gözlemlendi. Dama tahtası testi, klinik olarak ilgili patojenlere karşı hem NP'ler hem de geleneksel antibiyotikler arasında güçlü sinerjik etkiler gösterdi. FICI değerleri, çeşitli kombinasyonlarda tam sinerjiyi doğruladı ve membran geçirgenliğini artırarak ve bakteriyel direnç mekanizmalarına müdahale ederek antibiyotik etkilerini güçlendirme yeteneklerini gösterdi. YAgNP'ler ayrıca, IC₅₀ değerlerinin önemli ölçüde daha düşük olduğu HeLa ve HCT116 hücre hatlarına karşı kanser hücrelerine karşı önemli sitotoksik etkiler gösterdi ve onkolojik vaatlerini vurguladı. Genel olarak, çalışma yeşil sentezlenen nanopartiküllerin çeşitli terapötik uygulamalarını vurgular ve kanser tedavisinde ve antimikrobiyal ilaçlar için güçlendirici olarak potansiyel kullanımlarını önerir.

Abstract

Driven by the increasing pursuit of eco-friendly and multifunctional nanomaterials, this study explores the eco-conscious production, detailed analysis, and cooperative antibacterial efficacy of silver (YAgNPs) and copper nanoparticles (YCuNPs) synthesized using Morus nigra leaf extract under alkaline aqueous conditions. The nanoparticles produced through biogenic methods showed unique physical and chemical features, as validated via FTIR, XRD, TEM, and EDX instrumentation, suggesting effective reduction and stabilization facilitated by plant-derived compounds. YAgNPs and YCuNPs synthesized in this study demonstrated markedly enhanced antioxidant capacities and significant α-amylase and α-glucosidase inhibition, highlighting their prospective role in diabetes management. Antimicrobial assays showed superior activity of YAgNPs in solution-phase assays, whereas YCuNPs showed stronger inhibition in solid diffusion-based setups. Notably, lower MIC and MBC concentrations were observed for YAgNPs across multiple Gram-positive and Gram-negative strains, including E. coli and S. aureus. The checkerboard assay demonstrated potent synergistic effects between both NPs and traditional antibiotics against clinically relevant pathogens. FICI values confirmed full synergy in several combinations, demonstrating their ability to potentiate antibiotic effects through membrane permeability enhancement and interference with bacterial resistance mechanisms. YAgNPs also showed notable cytotoxic effects against cancer cells against HeLa and HCT116 cell lines, with IC₅₀ values significantly lower, highlighting their oncological promise. Overall, the study emphasizes the diverse therapeutic applications of green-synthesized nanoparticles and suggests their potential use in cancer treatment and as enhancers for antimicrobial drugs.

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Bu Makaleye Atıf Yap

Akmeşe, O., Baltacı, C., Gültekin, E., Taskın, I. I., Sever, M. R., Derin, D. Ç., Karpuz, Ö. (2025). Morus nigra L. Yaprağından Elde Edilen Fitojenik Gümüş ve Bakır Nanopartikülleri Çok İşlevli Ajanlar Olarak: Antioksidan, Antidiyabetik, Antimikrobiyal ve Antikanser Etkisi. Turkish Journal of Analytical Chemistry, Cilt: 7 Sayı: 3, 321–337. https://doi.org/10.51435/turkjac.1738363

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