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Dergiler / Current Perspectives on Medicinal and Aromatic Plants / 2022 / Volume: 5 Issue: 2

Anti-Acetylcholinesterase and Synergistic Antifungal Activities of Selected Salvia Species: Correlation with Metabolic Profiles

Gamze Benli Yardımcı(AFYONKARAHİSAR SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ)0000-0002-6469-8116
Nurnehir Baltacı(AFYONKARAHİSAR SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ)0000-0001-7054-8889
Çiğdem Kahraman(HACETTEPE ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ)0000-0001-8096-0738
Ekrem Murat Gönülalan(AFYONKARAHİSAR SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ)0000-0002-8171-3824
Sayfa
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Yayın Tarihi
2023-01-02
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Further studies are needed to investigate active metabolites according to correlation results.

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Amaç & Hipotez:Salvia species are known for their anti-fungal and anti-acetylcholinesterase (AChE) activity.
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Temel Bulgular & Veri:Also, Ellman's spectrophotometric method for anti-acetylcholinesterase activity and the checkerboard method for synergistic antifungal activity between extracts and fluconazole were performed. 295 known and 1408 unknown metabolites were detected in GC-MS while 346 known and 69008 unknown metabolites in LC-QTOF-MS.
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Sonuç & Katkı:Further studies are needed to investigate active metabolites according to correlation results.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Acetylcholinesterase inhibitory activity · Metabolomics · Salvia

Abstract

Salvia species are known for their anti-fungal and anti-acetylcholinesterase (AChE) activity. Metabolomics is defined as a comprehensive quantitative and qualitative analysis of large-scale metabolites. In this study, besides determining anti-acetylcholinesterase and synergistic antifungal activities of selected Salvia species (S. cryptantha, S. tchihatcheffii, S. officinalis, S. virgata), the metabolite profiles were clarified and correlation analyzes between the activity results and profiles were carried out. Aerial parts of all plant materials were extracted by methanol to determine the metabolic profile by using GC-MS (Gas Chromatography-Mass Spectrometry) and LC-QTOF-MS (Liquid Chromatography Quadrupole Time of Flight Mass Spectrometry). Also, Ellman's spectrophotometric method for anti-acetylcholinesterase activity and the checkerboard method for synergistic antifungal activity between extracts and fluconazole were performed. 295 known and 1408 unknown metabolites were detected in GC-MS while 346 known and 69008 unknown metabolites in LC-QTOF-MS. S. cryptantha, S. tchihatcheffii, S. officinalis, and S. virgata demonstrated inhibitory activities on AChE with the ratio of 32.72%, 5.9%, 43.96%, and 12.1% at the concentration of 200 µg/m and a synergism was found for all Salvia sp. against Candida tropicalis ATCC 750 strain with the FICI values of 0.28, 0.15, 0.10, 0.5 respectively. In addition, as a result of correlation analysis, 73 metabolites positively and 31 metabolites negatively correlated with anti-acetylcholinesterase activity, while 18 metabolites positively, and 117 metabolites negatively correlated with synergistic activity were found. All Salvia species exhibited synergic activity with fluconazole while having weak AChE inhibitory activity. Combining natural products with synthetic drugs is important in order to increase efficacy. At the same time, herbal products must be used carefully at the point of herb-drug interactions. Further studies are needed to investigate active metabolites according to correlation results.

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Bu Makaleye Atıf Yap

Yardımcı, G. B., Baltacı, N., Kahraman, Ç., Gönülalan, E. M. (2023). Anti-Acetylcholinesterase and Synergistic Antifungal Activities of Selected Salvia Species: Correlation with Metabolic Profiles. Current Perspectives on Medicinal and Aromatic Plants, Volume: 5 Issue: 2. https://doi.org/10.38093/cupmap.1209073

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