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Dergiler / Black Sea Journal of Engineering and Science / 2026 / Cilt: 8 Sayı: 1

Highly Up-Regulation of Fas Ligand Gene Expression after Increasing in Oxidized Low-Density Lipoprotein

Burcu Bayyurt(SİVAS CUMHURİYET ÜNİVERSİTESİ)0000-0002-5618-457X
Serdal Arslan(MERSİN ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-3921-8061
Sayfa
54–57
Yayın Tarihi
2025-01-15
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

According to our findings, we concluded that increasing the amount of ox-LDL may be critical in inducing the FASL-dependent apoptotic pathway in ECs.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Oxidized low-density lipoprotein (ox-LDL) shows many harmful effects such as induction of apoptosis on function of endothelial cell (EC).
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Temel Bulgular & Veri:FASL gene expression was statistic significantly up-regulated in the group treated higher amounts of ox-LDL (P<0.001).
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Sonuç & Katkı:According to our findings, we concluded that increasing the amount of ox-LDL may be critical in inducing the FASL-dependent apoptotic pathway in ECs.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Oxidized low-density lipoprotein · Fas ligand · Gene expression

Özet

Oxidized low-density lipoprotein (ox-LDL) shows many harmful effects such as induction of apoptosis on function of endothelial cell (EC). Fas ligand (FASL) induces apoptosis in divergent pathological conditions. EC apoptosis which induced by ox-LDL is related with FASL. In this study, we aimed to determine how different ox-LDL levels affect FASL expression in ECs. We treated human umbilical vein endothelial cells (HUVECs) with two different concentration of ox-LDL. FASL gene expression in groups was detected by quantitative polymerase chain reaction (qPCR). We compared FASL gene expression level between the groups according to ∆∆Ct method. FASL gene expression was statistic significantly up-regulated in the group treated higher amounts of ox-LDL (P<0.001). We found that treatment with higher amounts of ox-LDL in HUVECs increased FASL gene expression dramatically. According to our findings, we concluded that increasing the amount of ox-LDL may be critical in inducing the FASL-dependent apoptotic pathway in ECs.

Abstract

Oxidized low-density lipoprotein (ox-LDL) shows many harmful effects such as induction of apoptosis on function of endothelial cell (EC). Fas ligand (FASL) induces apoptosis in divergent pathological conditions. EC apoptosis which induced by ox-LDL is related with FASL. In this study, we aimed to determine how different ox-LDL levels affect FASL expression in ECs. We treated human umbilical vein endothelial cells (HUVECs) with two different concentration of ox-LDL. FASL gene expression in groups was detected by quantitative polymerase chain reaction (qPCR). We compared FASL gene expression level between the groups according to ∆∆Ct method. FASL gene expression was statistic significantly up-regulated in the group treated higher amounts of ox-LDL (P<0.001). We found that treatment with higher amounts of ox-LDL in HUVECs increased FASL gene expression dramatically. According to our findings, we concluded that increasing the amount of ox-LDL may be critical in inducing the FASL-dependent apoptotic pathway in ECs.

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Bu Makaleye Atıf Yap

Bayyurt, B., Arslan, S. (2025). Highly Up-Regulation of Fas Ligand Gene Expression after Increasing in Oxidized Low-Density Lipoprotein. Black Sea Journal of Engineering and Science, Cilt: 8 Sayı: 1, 54–57. https://doi.org/10.34248/bsengineering.1578992

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Kaynakça (17)

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