KaydedilenlerDerginizi ekleyin

Dergiler / Acta Veterinaria Eurasia / 2020 / Cilt: 46 - Sayı: 2

Effect of Cysteamine on the Freezing of Buck Semen

Ezgi ERTÜRK
Hatice ŞENLİKÇİ
Asiye İzem SANDAL
Özen Banu ÖZDAŞ
Kemal AK
Sayfa
63–67
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

In the equilibration stage, 10 mM cysteamine was found to damage the spermatozoa motility regardless of the presence of seminal plasma (p

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:This study investigated the effect of cysteamine added to an extender at different doses (5 and 10 mM) on the freezing of Saanen buck semen containing samples with seminal plasma or those in which the seminal plasma was removed.
📊
Temel Bulgular & Veri:Subsequent to the equilibration process, diluted semen samples were packaged in 0.25 mL straws, frozen at −110°C, and stored at −196°C.
💡
Sonuç & Katkı:In the equilibration stage, 10 mM cysteamine was found to damage the spermatozoa motility regardless of the presence of seminal plasma (p
🔬Tür:Laboratuvar & Saha Deneyi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

This study investigated the effect of cysteamine added to an extender at different doses (5 and 10 mM) on the freezing of Saanen buck semen containing samples with seminal plasma or those in which the seminal plasma was removed. After the examinations, the ejaculates were pooled. The semen was divided into two equal volumes. The seminal plasma of one group was not removed (Group A), whereas the seminal plasma of the other volume was removed via centrifugation (Group B). Each group was again divided into three equal volumes. Therefore, a total of six groups were created. Subsequent to the equilibration process, diluted semen samples were packaged in 0.25 mL straws, frozen at −110°C, and stored at −196°C. Frozen semen samples were thawed in a water bath for 30 s at 37°C. This procedure was repeated seven times (n=7). In the equilibration stage, 10 mM cysteamine was found to damage the spermatozoa motility regardless of the presence of seminal plasma (p

🔖

Bu Makaleye Atıf Yap

ERTÜRK, E., ŞENLİKÇİ, H., SANDAL, A. İ., ÖZDAŞ, Ö. B., AK, K. (2020). Effect of Cysteamine on the Freezing of Buck Semen. Acta Veterinaria Eurasia, Cilt: 46 - Sayı: 2, 63–67. https://ajindex.com/articles/141664

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (5)

  1. Ak, K., Cirit, Ü., Nur, Z., Bacınoğlu, S., Pabuccuoğlu, S., Özdaş, Ö.B., Birler, S., 2010. Effects of extender osmolarity, cooling rate, dilution rate and glycerol addition time on postthaw ram semen characteristics and fertilization. Acta Veterinaria Eurasia 36, 33-46.
  2. Alçay, S., Gökçe, E., Toker, M.B., Önder, N.T., Üstüner, B., Uzabacı, E., Gül, Z., Çavuş, S., 2016. Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa. Cryobiology 72, 269-273. [CrossRef] Bucak, M.N., Tuncer, P.B., Sarıözkan, S., Ulutaş, P.A., Çoyan, K., Başpınar, N., Özkalp, B., 2009. Effects of hypotaurine, cysteamine and aminoacids solution on post-thaw microscopic and oxidative stress parameters of Angora goat semen. Research in Veterinary Science 87, 468-472. [CrossRef] Cabrera, F., Gonzalez, F., Batista, M., Calero, P., Medrano, A., Gracia, A., 2005. The effect of removal of seminal plasma, egg yolk level and season on sperm freezability of canary buck (Capra hircus). Reproduction in Domestic Animals 40, 191-195. [CrossRef] Gordon, I.R., 2005. Artificial Insemination (Ed) In: Reproductive Technologies in Farm Animals. Cambridge, USA. [CrossRef] Hancock, J.L., 1952. The morphology of the bull spermatozoa. Journal of Experimental Biology 29, 445-453.
  3. Kozdrowski, R., Dubiel, A., Bielas, W., Dzielciol, M., 2007. Two protocols of cryopreservation of goat semen with the use of computer-assisted semen analysis system. Acta Veterinaria Brno 76, 601- 604. [CrossRef] Kulaksız, R., Daşkın, A., 2009. In vivo and in vitro evaluation of Saanen buck semen frozen with different antioxidants. Ankara Üniversitesi Veteriner Fakültesi Dergisi 56, 201-205. [CrossRef] Naing, S.W., Haron, A.W., Goriman, M.A.K., Yusoff, R., Bakar, Z.A., Sarsaifi, K., Bukar, M.M., Thein, M., Kyaw, T., San, M.M., 2011. Effect of seminal plasma removal, washing solutions, and centrifugation regimes on boer goat semen cryopreservation. Pertanika Journal of Tropical Agricultural Science 34, 271-279.
  4. Öztürkler, Y., Baran, A., Evecen, M., Ak, K., İleri, İ.K., 2001. Comparison of Ovine Spermatozoal Morphological Features After Staining of Fixation and Assessment of Morphological Abnormalities in Dead/Live Spermatozoa. The Turkish Journal of Veterinary and Animal Science 25, 675-680.
  5. Pellicer-Rubio, M.T., Magallon, T., Combarnous, Y., 1997. Deterioration of goat sperm viability in milk extenders is due to a bulbourethral 60-kilodalton glycop