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Dergiler / Çukurova Anestezi ve Cerrahi Bilimler Dergisi / 2023 / Cilt: 6 Sayı: 3

EFFECT OF RESVERATROL AND QUERCETİN ON INTESTİNAL ISCHEMİA REPERFUSİON INJURY İN RATS

Effect of Resveratrol and Quercetin on Intestinal Ischemia Reperfusion Injury in Rats

Işıl Yağmur(KAHRAMANMARAŞ SÜTÇÜ İMAM ÜNİVERSİTESİ)0000-0002-7009-4693
Selen Dindar(KAHRAMANMARAŞ SÜTÇÜ İMAM ÜNİVERSİTESİ)0000-0003-3780-8479
Rabia Tural(SINOP UNIVERSITY)0000-0003-3394-6890
Aslı Yaylalı(KAHRAMANMARAS SUTCU IMAM UNIVERSITY)0000-0003-2360-5320
Atila Yoldaş(KAHRAMANMARAS SUTCU IMAM UNIVERSITY)0000-0002-7807-0661
Fatma İnanç Tolun(KAHRAMANMARAS SUTCU IMAM UNIVERSITY)0000-0002-1157-2958
Hasan Dağlı(Kahramanmaraş Sütçü İmam University)0000-0003-2756-6277
Sayfa
467–472
Yayın Tarihi
2023-12-31
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Tartışma: Resveratrol ve quercetin intestinal I/R hasarında oksidatif stresin azaltılmasında koruyucu etkilidir.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Giriş: İntestinal iskemi reperfüzyon (İ/R) hasarı yüksek mortalite oranına sahip, erken tanısı çok zor olan acil bir durumdur.
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Temel Bulgular & Veri:Bu çalışmamızda resveratrol ve quercetin’in mezenter İ/R hasarı modeliyle oluşan intestinal hasardaki oksidatif stres ve antioksidan sistem üzerine etkilerinin biyokimyasal ve histopatolojik olarak incelenmesi amaçlanmıştır.
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Sonuç & Katkı:Tartışma: Resveratrol ve quercetin intestinal I/R hasarında oksidatif stresin azaltılmasında koruyucu etkilidir.
🔬Tür:Modelleme & Sayısal Simülasyon
📍Kapsam:56 adet
⚙️Değişkenler:İschemia-reperfusion · resveratrol · quercetin

Özet

Giriş: İntestinal iskemi reperfüzyon (İ/R) hasarı yüksek mortalite oranına sahip, erken tanısı çok zor olan acil bir durumdur. Bu çalışmamızda resveratrol ve quercetin’in mezenter İ/R hasarı modeliyle oluşan intestinal hasardaki oksidatif stres ve antioksidan sistem üzerine etkilerinin biyokimyasal ve histopatolojik olarak incelenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmamızda 250-300 gr ağırlığında 56 adet Sprague-Dawley cinsi erkek sıçan randomize bir şekilde her grupta 8’er sıçandan oluşan 7 gruba ayrılmıştır: kontrol grubu (grup 1), salin grubu (grup 2), etanol grubu (grup 3), resveratrol grubu (grup 4) (30mg/kg), quercetin grubu (grup 5) (30mg/kg), resveratrol+quercetin grubu A (grup 6) (15mg/kg+15 mg/kg), resveratrol+quercetin grubu B (grup 7) (30 mg/kg+30 mg/kg). Deney sonunda tüm ratlar sakrifiye edilerek bağırsak dokuları alınmış ve biyokimyasal ve histopatolojik inceleme için 2 parçaya ayrılmıştır. Total oksidan seviye (TOS), total antioksidan seviye (TAS), total tiyol [(-SH)+(-S-S-)] (TT), native tiyol [-SH] (NT) ve protein miktarı düzeyleri spektrofotometrik olarak ölçülmüş, oksidatif stres indeksi (OSI) ve disülfit [-S-S-] düzeyleri hesaplanmıştır. Sonuç: TOS, OSI, TT, NT ve disülfit düzeyleri açısından gruplar arasında istatistiksel olarak anlamlı fark gözlenirken (p<0.05), TAS düzeyleri açısından gruplar arasında istatistiksel olarak anlamlı fark gözlenmemiştir (p˃0.05). Histopatalojik skorlamada, tüm tedavi gruplarında salin ve etanol gruplarına göre anlamlı bir düzelme görülmüştür (p<0.05). Tartışma: Resveratrol ve quercetin intestinal I/R hasarında oksidatif stresin azaltılmasında koruyucu etkilidir.

Abstract

Aim: Intestinal ischemia reperfusion (I/R) injury is an emergency condition with a high mortality rate and early diagnosis is very difficult. In this study, we aimed to examine the biochemical and histopathological effects of resveratrol and quercetin on intestinal I/R injury model. Material and Method: In our study, 56 male Sprague-Dawley rats weighing 250-300 g were randomly divided into 7 groups consisting of 8 rats. Groups were control group (group 1), saline group (group 2), ethanol group (group 3), resveratrol group (group 4) (30mg/kg), quercetin group (group 5) (30mg/kg), resveratrol+quercetin group A (group 6) (15 mg/kg+15 mg/kg), resveratrol+quercetin group B (group 7) (30 mg/kg+30 mg/kg). At the end of the experiment rats intestinal tissues were divided into 2 parts for biochemical and histopathological examination. Total oxidant level (TOS), total antioxidant level (TAS), total thiol [(-SH)+(-S-S-)] (TT), native thiol [-SH] (NT), and protein content levels were measured spectrophotometrically, oxidative stress index (OSI) and disulfide [-S-S-] levels were calculated. Results: A statistically significant difference was found between the groups in terms of TOS, OSI, TT, NT and disulfide levels (p <0.05). No statistically significant difference was observed between the groups in terms of TAS levels (p˃0.05). A significant improvement in histopathological scoring was observed in all treatment groups compared to saline and ethanol groups (p<0.05). Conclusion: Resveratrol and quercetin have protective effects in reducing oxidative stress in intestinal I/R damage.

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Bu Makaleye Atıf Yap

Yağmur, I., Dindar, S., Tural, R., Yaylalı, A., Yoldaş, A., Tolun, F. İ., Dağlı, H. (2023). EFFECT OF RESVERATROL AND QUERCETİN ON INTESTİNAL ISCHEMİA REPERFUSİON INJURY İN RATS. Çukurova Anestezi ve Cerrahi Bilimler Dergisi, Cilt: 6 Sayı: 3, 467–472. https://doi.org/10.36516/jocass.1303016

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