Dergiler / Black Sea Journal of Agriculture / 2026 / Cilt: 9 Sayı: 5

Chemical and Functional Characterization of Soy Protein Concentrate Produced by an Ultrasound-Assisted Ethanol Washing Process

Ayberk Erdoğan(ADANA ALPARSLAN TÜRKEŞ BİLİM VE TEKNOLOJİ ÜNİVERSİTESİ)0009-0004-7650-7753
İrem İçöz(Sunar Mısır Entegre Tesisleri San. ve Tic. A.Ş.)0009-0002-9149-5477
Levent Yurdaer Aydemir(ADANA ALPARSLAN TÜRKEŞ BİLİM VE TEKNOLOJİ ÜNİVERSİTESİ)0000-0003-0372-1172
Sayfa
721–732
Yayın Tarihi
2026-09-15
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The results indicate that, under the selected processing conditions, ultrasound treatment mainly enhanced foaming behavior and the emulsion activity index, whereas its influence on protein enrichment and most other functional properties was limited; therefore, process conditions should be further optimized in terms of functional performance.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:In this study, soy protein concentrate (SPC) was produced from defatted soybean meal using ultrasound-assisted ethanol extraction, and the chemical composition and functional properties of the resulting product were investigated.
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Temel Bulgular & Veri:The effects of reaction time (2-4 h), solid-to-liquid ratio (1:4-1:12), and ethanol concentration (65-85%) were evaluated using a 2³ full factorial screening design.
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Sonuç & Katkı:The results indicate that, under the selected processing conditions, ultrasound treatment mainly enhanced foaming behavior and the emulsion activity index, whereas its influence on protein enrichment and most other functional properties was limited; therefore, process conditions should be further optimized in terms of functional performance.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Soy protein concentrate · Ultrasonic extraction · Ethanol extraction

Özet

In this study, soy protein concentrate (SPC) was produced from defatted soybean meal using ultrasound-assisted ethanol extraction, and the chemical composition and functional properties of the resulting product were investigated. The effects of reaction time (2-4 h), solid-to-liquid ratio (1:4-1:12), and ethanol concentration (65-85%) were evaluated using a 2³ full factorial screening design. The highest protein increase ratio (29.70%) was obtained in the screening run at 4 h, a 1:12 solid-to-liquid ratio, and 65% ethanol. Subsequent USPC and K-SPC samples were prepared under this selected condition; USPC contained 62.87±0.40% protein (wet basis; approximately 65.89% on a dry-matter basis). Ultrasound did not significantly increase total protein versus the control; it improved foaming capacity and short- to medium-term foam stability, with a more limited positive effect on the emulsion activity index. No marked improvement occurred in protein solubility, water-holding capacity, emulsion stability, or gelation. The results indicate that, under the selected processing conditions, ultrasound treatment mainly enhanced foaming behavior and the emulsion activity index, whereas its influence on protein enrichment and most other functional properties was limited; therefore, process conditions should be further optimized in terms of functional performance.

Abstract

In this study, soy protein concentrate (SPC) was produced from defatted soybean meal using ultrasound-assisted ethanol extraction, and the chemical composition and functional properties of the resulting product were investigated. The effects of reaction time (2-4 h), solid-to-liquid ratio (1:4-1:12), and ethanol concentration (65-85%) were evaluated using a 2³ full factorial screening design. The highest protein increase ratio (29.70%) was obtained in the screening run at 4 h, a 1:12 solid-to-liquid ratio, and 65% ethanol. Subsequent USPC and K-SPC samples were prepared under this selected condition; USPC contained 62.87±0.40% protein (wet basis; approximately 65.89% on a dry-matter basis). Ultrasound did not significantly increase total protein versus the control; it improved foaming capacity and short- to medium-term foam stability, with a more limited positive effect on the emulsion activity index. No marked improvement occurred in protein solubility, water-holding capacity, emulsion stability, or gelation. The results indicate that, under the selected processing conditions, ultrasound treatment mainly enhanced foaming behavior and the emulsion activity index, whereas its influence on protein enrichment and most other functional properties was limited; therefore, process conditions should be further optimized in terms of functional performance.

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Bu Makaleye Atıf Yap

Erdoğan, A., İçöz, İ., Aydemir, L. Y. (2026). Chemical and Functional Characterization of Soy Protein Concentrate Produced by an Ultrasound-Assisted Ethanol Washing Process. Black Sea Journal of Agriculture, Cilt: 9 Sayı: 5, 721–732. https://doi.org/10.47115/bsagriculture.1973714

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Kaynakça (23)

  1. AOAC. (1995a). Official method 923.03: Ash of flour. Association of Official Analytical Chemists.
  2. AOAC. (1995b). Official method 925.10: Solids (total) and loss on drying (moisture). Association of Official Analytical Chemists.
  3. AOAC. (1995c). Official method 960.52: Microchemical determination of nitrogen. Association of Official Analytical Chemists.
  4. AOAC. (1995d). Official method 991.43: Total, soluble and insoluble dietary fibre in foods. Association of Official Analytical Chemists.
  5. Aydemir, L. Y., & Yemenicioğlu, A. (2013). Potential of Turkish Kabuli type chickpea and green and red lentil cultivars as source of soy and animal origin functional protein alternatives. LWT - Food Science and Technology, 50(2), 686–694. https://doi.org/10.1016/j.lwt.2012.07.023
  6. Chemat, F., Zill-e-Huma, & Khan, M. K. (2011). Applications of ultrasound in food technology: Processing, preservation and extraction. Ultrasonics Sonochemistry, 18(4), 813–835. https://doi.org/10.1016/j.ultsonch.2010.11.023
  7. Damodaran, S. (1997). Food proteins: An overview. In S. Damodaran & A. Paraf (Eds.), Food proteins and their applications. Marcel Dekker.
  8. Damodaran, S. (2008). Amino acids, peptides, and proteins. In S. Damodaran, K. L. Parkin, & O. R. Fennema (Eds.), Fennema's Food Chemistry (4th ed.). CRC Press.
  9. Endres, J. G. (2001). Soy protein products: Characteristics, nutritional aspects, and utilization. AOCS Press.
  10. Eze, O. F., Chatzifragkou, A., & Charalampopoulos, D. (2022). Properties of protein isolates extracted by ultrasonication from soybean residue (okara). Food Chemistry, 368, 130837. https://doi.org/10.1016/j.foodchem.2021.130837
  11. Hu, H., Li-Chan, E. C. Y., Wan, L., Tian, M., & Pan, S. (2013). The effect of high intensity ultrasonic pre-treatment on the properties of soybean protein isolate gel induced by calcium sulfate. Food Hydrocolloids, 32(2), 303–311. https://doi.org/10.1016/j.foodhyd.2013.01.016
  12. Jambrak, A. R., Lelas, V., Mason, T. J., Krešić, G., & Badanjak, M. (2009). Physical properties of ultrasound treated soy proteins. Journal of Food Engineering, 93(4), 386–393. https://doi.org/10.1016/j.jfoodeng.2009.02.001
  13. Kinsella, J. E. (1979). Functional properties of soy proteins. Journal of the American Oil Chemists’ Society, 56, 242–258.
  14. Kong, Y., Sun, L., Wu, Z., Li, Y., Kang, Z., Xie, F., & Yu, D. (2023). Effects of ultrasonic treatment on the structural, functional properties and beany flavor of soy protein isolate: Comparison with traditional thermal treatment. Ultrasonics Sonochemistry, 101, 106675. https://doi.org/10.1016/j.ultsonch.2023.106675
  15. Liu, K. (1997). Soybeans: Chemistry, technology, and utilization. Chapman & Hall.
  16. Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193(1), 265–275.
  17. Mason, T. J., & Lorimer, J. P. (2002). Applied sonochemistry: Uses of power ultrasound in chemistry and processing. Wiley-VCH.
  18. Peng, Y., Kyriakopoulou, K., Ndiaye, M., Bianeis, M., Keppler, J. K., & van der Goot, A. J. (2021). Characteristics of soy protein prepared using an aqueous ethanol washing process. Foods, 10(9), 2222. https://doi.org/10.3390/foods10092222
  19. Phillips, G. O., & Williams, P. A. (2011). Introduction to food proteins. In G. O. Phillips & P. A. Williams (Eds.), Handbook of food proteins. Woodhead Publishing.
  20. Preece, K. E., Hooshyar, N., Krijgsman, A., Fryer, P. J., & Zuidam, N. J. (2017). Intensified soy protein extraction by ultrasound. Chemical Engineering and Processing: Process Intensification, 113, 94–101. https://doi.org/10.1016/j.cep.2016.09.003
  21. Rahman, M. M., & Lamsal, B. P. (2021). Ultrasound-assisted extraction and modification of plant-based proteins: Impact on physicochemical, functional, and nutritional properties. Comprehensive Reviews in Food Science and Food Safety, 20(2), 1457–1480. https://doi.org/10.1111/1541-4337.12709
  22. Riaz, M. N. (2006). Soy applications in food. CRC Press.
  23. Wang, T., Wang, N., Li, N., Ji, X., Zhang, H., Yu, D., & Wang, L. (2022). Effect of high-intensity ultrasound on the physicochemical properties, microstructure, and stability of soy protein isolate-pectin emulsion. Ultrasonics Sonochemistry, 82, 105871. https://doi.org/10.1016/j.ultsonch.2021.105871