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Dergiler / Harran Tarım ve Gıda Bilimleri Dergisi / 2026 / Cilt: 16 Sayı: 1

SICAKLIK STRESİ ALTINDAKİ ÇİLEK BİTKİLERİNDE H2O2 VE PEROKSİDAZ AKTİVİTESİNDEKİ DEĞİŞİMLER

CHANGES IN H2O2 AND PEROXIDASE ACTIVITIES IN STRAWBERRY PLANTS UNDER HEAT STRESS

Serpil Ergin
Müge Kesici
Hatice Gülen
Sayfa
25–35
Yayın Tarihi
2014-02-20
DOI
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

NativePAGE’de 60ºC haricindeki bütün sıcaklıklarda bazik bir peroksidaz bandı tespit edilmiştir.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Yüksek sıcaklık stresinin etkileri, yüksek sıcaklığa tolerant ve hassas olan Redlands Hope (R.
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Temel Bulgular & Veri:Hope) ve Cal-Giant 3 (CG3) çilek çeşitlerinde araştırılmıştır.
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Sonuç & Katkı:NativePAGE’de 60ºC haricindeki bütün sıcaklıklarda bazik bir peroksidaz bandı tespit edilmiştir.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Çilek · Fragaria x ananassa · yüksek sıcaklık

Özet

Yüksek sıcaklık stresinin etkileri, yüksek sıcaklığa tolerant ve hassas olan Redlands Hope (R. Hope) ve Cal-Giant 3 (CG3) çilek çeşitlerinde araştırılmıştır. Bitkilerden alınan yaprak örnekleri kademeli olarak 35, 40, 43, 46, 49, 52, 55 ve 60°C sıcaklıklara maruz bırakılıp “kademeli yüksek sıcaklık stresi” oluşturulmuştur. Ayrıca, bitkilerden alınan yaprak örnekleri “şok yüksek sıcaklık stresi” oluşturmak amacıyla doğrudan bu sıcaklıklara maruz bırakılmışlardır. Her bir sıcaklık uygulamasında iyon sızıntısı, hidrojen peroksit (H2O2) ve peroksidaz (PRX) aktivitesi incelenmiştir. Kademeli ve şok yüksek sıcaklık uygulamalarının her ikisinde de iyon sızıntısı ve H2O2 miktarının CG3 çeşidinde R.Hope’a gore daha yüksek olduğu belirlenmiştir. NativePAGE’de 60ºC haricindeki bütün sıcaklıklarda bazik bir peroksidaz bandı tespit edilmiştir. Belirlenen bandın yoğunluğunun CG3’te R.Hope’a gore daha yüksek olduğu görülmüştür. Sonuçta, yüksek sıcaklığa tolerant olan R.Hope’da PRX aktivitesinin düşük olmasına rağmen, H2O2 birikiminin ve hücresel zararlanmanın da düşük olması, diğer savunma sistemleri ile ilişkilendirilmiştir

Abstract

Effects of heat stress were investigated in two strawberry cultivars, Redlands Hope (R. Hope) and Cal-Giant 3 (CG3), which are heat-tolerant and heat-sensitive, respectively. Collected leaves from the grown plants were exposed to high temperature increased gradually to 35, 40, 43, 46, 49, 52, 55 and 60 ° C to impose a “gradual heat stress” (GHS). Additional leaves collected from the plants were also exposed directly to each temperature, to impose a “shock heat stress” (SHS). Electrolyte leakage, hydrogen peroxide (H2O2) and peroxidase (PRX) activity were evaluated fallowing each temperature. The electrolyte leakage and H2O2 levels were found higher in CG3 than R. Hope in both GHS and SHS treatments. A basic PRX band was observed in all treatments except 60ºC on native PAGE with different intensities. The intensities of the band were generally higher in CG3 than R. Hope. In conclusion less H2O2 accumulation and cellular damage in heat tolerant cv. R. Hope in spite of less PRX activity can be correlated with the effects of other defense systems

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Bu Makaleye Atıf Yap

Ergin, S., Kesici, M., Gülen, H. (2014). SICAKLIK STRESİ ALTINDAKİ ÇİLEK BİTKİLERİNDE H2O2 VE PEROKSİDAZ AKTİVİTESİNDEKİ DEĞİŞİMLER. Harran Tarım ve Gıda Bilimleri Dergisi, Cilt: 16 Sayı: 1, 25–35. https://ajindex.com/articles/1348904

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Kaynakça (186)

  1. Arora, A., Sairam, R.K. and Srivastava, G.C.
  2. Oxidative stress and antioxidative
  3. system in plants. Current Science, 82
  4. :1227-1238.
  5. Arora, R., Pitchay, D.S. and Bearce, B.C. 1998.
  6. Water-stress-induced heat tolerance in
  7. geranium leaf tissues: a possible linkage
  8. through stres proteins. Phsiol. Plant.,: 24-34.
  9. Chaitanya, K.V., Sundar, D., Masilamani, S.
  10. and Reddy, A.R. 2002. Variation in heat
  11. stres-induced antioxidant enzyme
  12. activities among three mulberry
  13. cultivars. Plant Growth Regul., 36: 175-
  14. Davis, B.J. 1964. Disc electrophoresis. Method
  15. and application to human serum
  16. proteins. Ann. NY. Acad. Sci., 121: 404-
  17. Foyer, C.H., Descourvieres. P. and Kunert, K.
  18. Protection against oxygen
  19. radicals: important defence mechanisms
  20. studied in transgenic plants. Plant Cell
  21. Environ., 17: 507-523.
  22. Premachandra, G.S., Saneoka, H., Fujita, K.
  23. and Ogata, S. 1992. Leaf water
  24. relations, osmotic adjustment, cell
  25. membrane stability, epi-cuticular wax
  26. load and growth as affected by
  27. increasing water deficits in Sorghum, J.
  28. Exp. Bot., 43: 1569-1576.
  29. Gaspar, T.H., Penel, C.L., Thorpe, T. and
  30. Grappin, H. 1982. Peroxidases a survey
  31. of their biochemical and physiological
  32. roles in higher plants. Unıversıté De
  33. Genève Press., Genève. pp. 10-60.
  34. Gulen, H., Arora, R., Kuden, A., Krebs, S.L.
  35. and Postman, J. 2002. Peroxidase
  36. isozyme profiles in compatible and
  37. incompatible pear-quince graft
  38. combinations. J. Amer. Soc. Hort. Sci.,: 152-157.
  39. Gulen, H. and Eris, A. 2003. Some
  40. physiological changes in strawberry
  41. (Fragaria x ananassa cv. Camarosa)
  42. plants under heat stres. J. Hort. Sci.,
  43. Biotech. 78: 894-898.
  44. Gulen, H. and Eris, A. 2004. Effect of heat
  45. stress on peroxidase activity and total
  46. protein content in strawberry plants.
  47. Plant Sci., 166: 739-744.
  48. Chen, H.H., Shen, Z.V. and Li, P.H. 1982.
  49. Adaptability of crop plants to high
  50. temperature stress. Crop Sci., 22:719-
  51. Havaux, M. 1993. Rapid photosynthetic
  52. adaptation to heat stress triggered in
  53. potato leaves by moderately elevated
  54. temperatures. Plant Cell Environ., 16:
  55. Howarth, C.J. 2005. Genetic improvements of
  56. tolerance to high temperature.
  57. “Alınmıştır: Abiotic Stresses: Plant
  58. Resistance Through Breeding and
  59. Molecular Approaches. (Eds.) Ashraf,
  60. M., Haris, P.J.C. Howarth Pres. Inc.,
  61. NewYork.”
  62. Huystee, R.B.V. 1987. Some molecular aspects
  63. of plant peroxidase biosynthetic studies.
  64. Annu. Review Plant Physiol. 38: 205-
  65. J.Agric.Fac.HR.U., 2012,16(1) Ergin et al.
  66. Jeffrey, A.A. 2002. Catalase activity, hydrogen
  67. peroxide content and thermotolerance of
  68. pepper leaves. Sci. Hort., 95: 277–284.
  69. Kesici, M. 2009. High Temperature Tolerance
  70. of Some Strawberry (Fragaria 
  71. ananassa) Cultivars. (In Turkish with
  72. English summary). MSc Thesis, Uludag
  73. Univ. Inst. Natural Sci.,Bursa, Turkey.
  74. Kesici, M., Ergin, S., Gulen, H., Turhan, E.,
  75. Ipek, A. and Koksal, N. 2012. Heatstress
  76. tolerance of some strawberry
  77. (Fragaria x ananassa) cultivars.
  78. Outlook Agr. (in press).
  79. Kocsy, G., Szalai, G., Sutka, J., Paldi, E. and
  80. Galiba, G. 2004. Heat tolerance together
  81. with heat stress-induced changes in
  82. glutathione and hydroxyl methyl
  83. glutathione levels is affected by
  84. chromosome 5A of wheat. Plant Sci.,: 451-458.
  85. Mazorra, L.M., Nunez, M., Hechavarria, M.,
  86. Coll, F. and Sanchez-Blanco, M.J. 2002.
  87. Influence of brassinosteroids on
  88. antioxidant enzymes activity in tomato
  89. under different temperatures, Biol.
  90. Plant., 45: 593-596.
  91. Ledesma, N.A., Kawabata, S. and Sugiyama,
  92. N. 2004. Effect of high temperature on
  93. protein expression in strawberry plants.
  94. Biol. Plant., 481: 73-79.
  95. Ledesma, N.A., Nakata, M. and Sugiyama, N.
  96. Effect of high temperature stress
  97. on the reproductive growth of
  98. strawberry cvs. ‘Nyoho’ and
  99. ‘Toyonoka’. Sci. Hort., 116: 186-193.
  100. Lester, G.E. 1985. Leaf cell membrane
  101. thermostabilities of Cucumis melo. J.
  102. Amer. Soc. Hort. Sci., 110: 506- 509.
  103. Liu, X. and Huang, B. 2000. Heat stress injury
  104. in relation to membrane lipid
  105. peroxidation in creeping bentgrass.
  106. Crop Sci., 40: 503-513.
  107. Manganaris, A.G. and Alston, F.H. 1992.
  108. Inheritance and linkage relationships of
  109. peroxidase isozymes in apple. Theor.Appl. Genet., 83:392-399.
  110. Nakano, Y. and Asada, K. 1981. Hydrogen
  111. peroxide is scavenged by ascorbatespecific
  112. peoxidase in spinach
  113. chloroplasts. Plant Cell Physiol., 22:
  114. Ngo, T.T. and Lenhoff, H.M. 1980. A sensitive
  115. and versatile chromogenic assay for
  116. peroxidase and peroxidase-coupled
  117. reactions. Analytical Biochem.,:389-397.
  118. Okuda, T., Matsuda, Y., Yamanaka, A. and
  119. Sagisaka, S. 1991. Abrupt increase in
  120. level of hydrogen peroxide in leaves of
  121. winter wheat is caused by cold
  122. treatment. Plant Physiol., 97:1265-
  123. Reisfeld, R.A., Lewis, U.J. and Williams, D.E.
  124. Disk electrophoresis of basic
  125. proteins and peptides on polyacrylamide
  126. gels. Nature., 195: 281-283.
  127. Rivero, R.M., Ruiz, J.M. and Romero, L. 2003.
  128. Influence of temperature on biomass,
  129. iron metabolism and some related
  130. bioindicators in tomato and watermelon
  131. plants. J. Plant Physiol., 160: 1065-
  132. Saelim, S. and Zwiazek, J.J. 2000. Preservation
  133. of thermal stability of cell membranes
  134. and gas exchange in high temperatureacclimated
  135. Xylia xylocarpa seedlings,
  136. J.Plant Phsiol., 156: 380-385.
  137. Sairam, R.K., Srivastava, G.C. and Saxena,
  138. D.C. 2000. Increased antioxidant
  139. activity under elevated temperature: a
  140. mechanism of heat stress tolerance in
  141. wheat genotypes. Biol. Plant., 43: 245–
  142. Wahid, A., Gelani, S., Ashraf, M. and Foolad,
  143. M.R. 2007. Heat tolerance in plants: an
  144. overview. Environ. Exper. Botany.,:199-223.
  145. Walter, M.H. 1992. Regulation of lignification
  146. in defense. “Alınmıştır: Plant Gene
  147. Research: Genes Involved in Plant
  148. Defense. (Eds) Boller, T., Meins, F.
  149. Springer, Vienna, pp 327-352”
  150. Wang, S.Y. and Lin, H.S. 2006. Effect of plant
  151. growth temperature on membrane lipids
  152. in strawberry (Fragaria × ananassa
  153. Duch.). Scienta Hort., 108: 35-42
  154. Wang, S.Y. and Zheng, W. 2001. Effect of
  155. plant growth temperature on antioxidant
  156. capacity in strawberry. J. Agric. Food
  157. Chem., 49: 4977–4982.
  158. Wendel, J.F. and Weeden, N.F. 1989.
  159. Visualization and interpretation of plant
  160. isozymes. “Alınmıştır: Isozymes in
  161. Plant Biology, (Eds.) Soltis, D.E. and
  162. Soltis, P.S. Dioscorides Press, Portland,
  163. Oregon, pp. 5-44”
  164. Xu, S., Li, J., Zhang, X., Wei, H. and Cui, L.
  165. Effects of heat acclimation
  166. pretreatment on changes of membrane
  167. lipid peroxidation, antioxidant
  168. metabolites, and ultrastructure of
  169. chloroplasts in two cool-season
  170. turfgrass speciaes under heat stress.
  171. Environ. Exper. Botany., 56: 274-285.
  172. Yin, H., Chen, Q. and Yi, M. 2008. Effects of
  173. short-term heat stress on oxidative
  174. damage and responses of antioxidant
  175. system in Lilium longiflorum. Plant
  176. Growth Regul., 54:45–54.
  177. Yoshida, K., Kaothien, P., Matsui, T.,
  178. Kawaoka, A. and Shinmyo, A. 2003.
  179. Molecular biology and application of
  180. plant peroxidase genes. Appl Microbiol
  181. Biotech,. 60:665–670.
  182. Zhau, R.G., Fan, Z.H., Li, X.Z., Wang, Z.W.
  183. and Han, W. 1995. The effect of heat
  184. acclimation on membrane thermostability
  185. and reactive enzyme activity.
  186. Acta Agron. Sin., 21:568–572.