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Dergiler / Hitit Medical Journal / 2026 / Cilt: 8 Sayı: 1

Betülinik Asidin Renal Hücreli Karsinom Hücre Hattında GPX4 ve SLC7A11 Gen Ekspresyonu Üzerine Etkisi

Effect of Betulinic Acid on GPX4 and SLC7A11 Gene Expression in Renal Cell Carcinoma Cell Line

Leyla Acar İlvan(ISTANBUL UNIVERSITY, INSTITUTE OF HEALTH SCIENCES)0000-0002-0281-1785
Sibel Kuraş(UNIVERSITY OF HEALTH SCIENCES, SCHOOL OF MEDICINE)0000-0002-1230-7777
Merve Nur Ataş(ISTANBUL UNIVERSITY, AZIZ SANCAR INSTITUTE OF EXPERIMENTAL MEDICINE)0000-0001-5045-5095
Hayriye Arzu Ergen(ISTANBUL UNIVERSITY, AZIZ SANCAR INSTITUTE OF EXPERIMENTAL MEDICINE)0000-0001-5736-8453
Sayfa
28–35
Yayın Tarihi
2026-02-28
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Conclusion: Our findings suggest that betulinic acid may play a role in renal cell carcinoma line, especially through the glutathione mechanism.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Aim: Renal carcinoma is the predominant form of kidney cancer, with its frequency rising in recent years; thus, the advancement of novel therapeutic strategies is crucial due to its resistance to therapy in advanced stages.
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Temel Bulgular & Veri:This study aimed to examine the effect of betulinic acid on the expression levels of the glutathione peroxidase 4 and solute carrier family 7 member 11 genes related to glutathione metabolism in the renal cell carcinoma cell line CAKI-2 and the healthy cell line MRC-5.
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Sonuç & Katkı:Conclusion: Our findings suggest that betulinic acid may play a role in renal cell carcinoma line, especially through the glutathione mechanism.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Renal cell carcinoma · betulinic acid · glutathione metabolism

Özet

Aim: Renal carcinoma is the predominant form of kidney cancer, with its frequency rising in recent years; thus, the advancement of novel therapeutic strategies is crucial due to its resistance to therapy in advanced stages. Currently, investigations into the anticancer effects of natural substances are increasing. Betulinic acid is a lupan-type pentacyclic triterpenoid molecule found in various plants that is notable for its apoptotic and antioxidant properties. This study aimed to examine the effect of betulinic acid on the expression levels of the glutathione peroxidase 4 and solute carrier family 7 member 11 genes related to glutathione metabolism in the renal cell carcinoma cell line CAKI-2 and the healthy cell line MRC-5. Material and Methods: Expression analysis of GPX4 and SLC7A11 genes was performed by quantitative real time-polymerase chain reaction. The glyceraldehyde 3-phosphate dehydrogenase gene was used as housekeeping gene. Results: A significant increase in solute carrier family 7 member 11 (p=0.014) and glutathione peroxidase 4 (p=0.049) gene expression levels were observed due to 25µM betulinic acid treatment. Conclusion: Our findings suggest that betulinic acid may play a role in renal cell carcinoma line, especially through the glutathione mechanism.

Abstract

Aim: Renal carcinoma is the predominant form of kidney cancer, with its frequency rising in recent years; thus, the advancement of novel therapeutic strategies is crucial due to its resistance to therapy in advanced stages. Currently, investigations into the anticancer effects of natural substances are increasing. Betulinic acid is a lupan-type pentacyclic triterpenoid molecule found in various plants that is notable for its apoptotic and antioxidant properties. This study aimed to examine the effect of betulinic acid on the expression levels of the glutathione peroxidase 4 and solute carrier family 7 member 11 genes related to glutathione metabolism in the renal cell carcinoma cell line CAKI-2 and the healthy cell line MRC-5. Material and Methods: Expression analysis of GPX4 and SLC7A11 genes was performed by quantitative real time-polymerase chain reaction. The glyceraldehyde 3-phosphate dehydrogenase gene was used as housekeeping gene. Results: A significant increase in solute carrier family 7 member 11 (p=0.014) and glutathione peroxidase 4 (p=0.049) gene expression levels were observed due to 25µM betulinic acid treatment. Conclusion: Our findings suggest that betulinic acid may play a role in renal cell carcinoma line, especially through the glutathione mechanism.

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Bu Makaleye Atıf Yap

İlvan, L. A., Kuraş, S., Ataş, M. N., Ergen, H. A. (2026). Betülinik Asidin Renal Hücreli Karsinom Hücre Hattında GPX4 ve SLC7A11 Gen Ekspresyonu Üzerine Etkisi. Hitit Medical Journal, Cilt: 8 Sayı: 1, 28–35. https://doi.org/10.52827/hititmedj.1730449

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