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Dergiler / Troia Tıp Dergisi / 2026 / Cilt: 7 Sayı: 3

LPS kaynaklı sepsiste oksidan-antioksidan sistem değişiklikleri: Pregabalinin modülatör etkileri

Oxidant–antioxidant system alterations in LPS-induced sepsis: Modulatory effects of pregabalin

Dilek Çevik(SÜLEYMAN DEMİREL ÜNİVERSİTESİ)0000-0001-5328-3663
Nurhan Gümral(Süleyman Demirel Üniversitesi)0000-0002-8141-4533
Rahime Aslankoç(Süleyman Demirel Üniversitesi)0000-0001-9516-0864
Elif Selin Karanfil(SÜLEYMAN DEMİREL ÜNİVERSİTESİ)0009-0003-8544-3598
Ayça Coşkun(SÜLEYMAN DEMİREL ÜNİVERSİTESİ)0009-0004-7834-7036
Rumeysa Yorulmaz(SÜLEYMAN DEMİREL ÜNİVERSİTESİ)0009-0002-0364-9633
Sayfa
160–166
Yayın Tarihi
2026-09-30
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Bulgular: Beyin dokusunda PRG uygulanan grupta MDA düzeylerinin kontrol (p < 0.001) ve LPS (p = 0.029) gruplarına kıyasla istatistiksel olarak anlamlı derecede azaldığı saptandı.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Amaç: Bu çalışmanın amacı, lipopolisakkarit (LPS) ile indüklenen sepsis modelinde pregabalinin (PRG) oksidan–antioksidan sistem üzerindeki etkilerini beyin, pankreas, dalak ve testis dokularında araştırmaktır.
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Temel Bulgular & Veri:Kontrol grubuna in-traperitoneal (i.p.) ve oral yolla eşdeğer hacimlerde salin uygulandı.
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Sonuç & Katkı:Bulgular: Beyin dokusunda PRG uygulanan grupta MDA düzeylerinin kontrol (p < 0.001) ve LPS (p = 0.029) gruplarına kıyasla istatistiksel olarak anlamlı derecede azaldığı saptandı.
🔬Tür:Deneysel Tarla Denemesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Sepsis · Pregabalin · Oksidan-Antioksidan Sistem

Özet

Amaç: Bu çalışmanın amacı, lipopolisakkarit (LPS) ile indüklenen sepsis modelinde pregabalinin (PRG) oksidan–antioksidan sistem üzerindeki etkilerini beyin, pankreas, dalak ve testis dokularında araştırmaktır. Yöntem: Sıçanlar rastgele kontrol, LPS ve LPS+PRG olmak üzere üç gruba ayrıldı. Kontrol grubuna in-traperitoneal (i.p.) ve oral yolla eşdeğer hacimlerde salin uygulandı. LPS grubunda sepsis, 5 mg/kg dozunda i.p. LPS uygulanarak oluşturuldu. LPS+PRG grubuna, LPS uygulamasından 1 saat önce oral yolla 30 mg/kg pregabalin verildi; bunu takiben 1 saat sonra 5 mg/kg dozunda i.p. LPS uygulandı. Tüm hayvanlar, LPS uygulamasından 6 saat sonra sakrifiye edildi. Oksidan–antioksidan durumun değer-lendirilmesi amacıyla beyin, pankreas, dalak ve testis dokularında malondialdehit (MDA), katalaz (CAT) ve süperoksit dismutaz (SOD) düzeyleri ölçüldü. Bulgular: Beyin dokusunda PRG uygulanan grupta MDA düzeylerinin kontrol (p < 0.001) ve LPS (p = 0.029) gruplarına kıyasla istatistiksel olarak anlamlı derecede azaldığı saptandı. Kontrol grubuna kıyasla PRG uygulanan grupta CAT düzeyleri de anlamlı ölçüde azalırken (p = 0.040), LPS grubuna kıyasla istatistiksel açıdan anlamlı bir fark gözlenmedi (p = 0.240). Buna karşılık, SOD düzeyleri PRG uygulanan grupta hem kontrol hem de LPS gruplarına kıyasla istatistiksel olarak anlamlı düzeyde arttı (her iki karşılaştırma için p < 0.001). Pankreas, dalak ve testis dokularında ise istatistiksel olarak anlam-lı bir farklılık saptanmadı. Sonuç: PRG, LPS ile indüklenen sistemik inflamasyon sırasında beyin dokusundaki oksidan-antioksidan sistemini modüle edebilir. Pankreas, dalak ve testis dokuları üzerindeki olası etkilerinin aydınlatılabilmesi için daha ileri çalışmalara ihtiyaç vardır.

Abstract

Objective: This study aimed to investigate the effects of pregabalin (PRG) on the oxidant–antioxidant system in brain, pancreas, spleen, and testicular tissues using a lipopolysaccharide (LPS)-induced sepsis model. Methods: Rats were randomly assigned to three groups: control, LPS, and LPS+PRG. The control group received equivalent volumes of saline intraperitoneally (i.p.) and orally. Sepsis was induced in the LPS group by administering LPS (5 mg/kg, i.p.). In the LPS+PRG group, PRG was administered orally at a dose of 30 mg/kg 1 hour before LPS injection; subsequently, LPS (5 mg/kg, i.p.) was administered. All rats were sacrificed 6 hours after LPS administration. Malondialdehyde (MDA), catalase (CAT), and su-peroxide dismutase (SOD) levels were measured in brain, pancreas, spleen, and testicular tissues to eval-uate the oxidant–antioxidant status. Results: In brain tissue, no statistically significant differences in MDA, CAT, or SOD levels were ob-served between the control and LPS groups (p > 0.05). MDA levels were significantly decreased in the PRG-treated group compared with both the control (p < 0.001) and LPS (p = 0.029) groups. CAT levels were also significantly reduced in the PRG-treated group compared with the control group (p = 0.040), whereas no statistically significant difference was observed compared to the LPS group (p = 0.240). In contrast, SOD levels were significantly increased in the PRG-treated group compared with both the con-trol (p < 0.001) and LPS (p < 0.001) groups. No statistically significant differences were observed in pancreas, spleen, or testicular tissues. Conclusion: PRG may modulate the oxidant–antioxidant system in brain tissue during LPS-induced systemic inflammation. Further studies are required to clarify its effects on the pancreas, spleen, and testicular tissues.

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Bu Makaleye Atıf Yap

Çevik, D., Gümral, N., Aslankoç, R., Karanfil, E. S., Coşkun, A., Yorulmaz, R. (2026). LPS kaynaklı sepsiste oksidan-antioksidan sistem değişiklikleri: Pregabalinin modülatör etkileri. Troia Tıp Dergisi, Cilt: 7 Sayı: 3, 160–166. https://doi.org/10.55665/troiamedj.1862825

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