Dergiler / Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries / 2023 / Cilt: 6 Sayı: 2

Altınbaş Kefal (Chelon auratus) Pullarından Asitte Çözünür Kollajen Ekstraksiyonu ve Karakterizasyonu

Extraction and Characterization of Acid Soluble Collagen From Golden Grey Mullet (Chelon auratus) Scale

Cansu Metin Hacisa(MUĞLA SITKI KOÇMAN ÜNİVERSİTESİ)0000-0002-2290-1489
Taçnur Baygar(MUĞLA SITKI KOÇMAN ÜNİVERSİTESİ)0000-0001-8070-0653
Sayfa
166–177
Yayın Tarihi
2023-12-27
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Sonuç olarak, balık pulu atıklarından, doğal atıkların veya çevre sorunlarının yönetimine katkı sağlayacak, memeli kollajenine alternatif, gıda ve kozmetik gibi endüstriyel alanlarda kullanılabilecek bir kaynak elde edilmiştir.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Bu çalışmada değerli bir biyoaktif materyal elde etmek amacıyla atık balık pullarının potensiyali incelenmiştir.
📊
Temel Bulgular & Veri:Altınbaş kefal (Chelon auratus) pullarından asitte çözünür kollajen izole edilerek karakterize edilmiştir.
💡
Sonuç & Katkı:Sonuç olarak, balık pulu atıklarından, doğal atıkların veya çevre sorunlarının yönetimine katkı sağlayacak, memeli kollajenine alternatif, gıda ve kozmetik gibi endüstriyel alanlarda kullanılabilecek bir kaynak elde edilmiştir.
🔬Tür:Laboratuvar & Saha Deneyi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Biyoaktif madde · Asitte çözünür kollajen · Balık pulu

Özet

Bu çalışmada değerli bir biyoaktif materyal elde etmek amacıyla atık balık pullarının potensiyali incelenmiştir. Altınbaş kefal (Chelon auratus) pullarından asitte çözünür kollajen izole edilerek karakterize edilmiştir. Ekstrakte edilen kollajenin besin kompozisyonu, denatürasyon sıcaklığı, Sodyum dodesil sülfat jel elektroforezi (SDS-PAGE), amino asit bileşimi, Taramalı elektron mikroskobu ve Enerji dağılımlı X‑ışınları spektrometre (SEM-EDS), Fourier dönüşümü kızılötesi spektrofotometre (FTIR), Termogravimetrik analiz (TGA), antioksidan ve antimikrobiyal aktivite analizleri yapılarak ticari kollajenle karşılaştırılmıştır. Sonuç olarak, balık pulu atıklarından, doğal atıkların veya çevre sorunlarının yönetimine katkı sağlayacak, memeli kollajenine alternatif, gıda ve kozmetik gibi endüstriyel alanlarda kullanılabilecek bir kaynak elde edilmiştir.

Abstract

In the present study, the potential of waste fish scales for obtaining a valuable bioactive material was evaluated. Acid soluble collagen from golden grey mullet (Chelon auratus) scales was isolated and characterized successfully. Proximate composition, denaturation temperature, Sodium dodecyl sulphate gel electrophoresis (SDS-PAGE), amino acid composition, Scanning electron microscopy and Energy dispersive X‑ray spectroscopy (SEM-EDS), Fourier transform infrared spectrophotometer (FTIR), Thermogravimetric analysis (TGA), antioxidant and antimicrobial activity analyses were performed for the extraction of collagen and extracted collagen was compared with commercial collagen. As a result, an alternative and useful source for mammalian collagen for the industrial applications like food and cosmetics was extracted from the fish scale waste material, which may help mitigate the management of natural wastes or environmental problems.

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Bu Makaleye Atıf Yap

Hacisa, C. M., Baygar, T. (2023). Altınbaş Kefal (Chelon auratus) Pullarından Asitte Çözünür Kollajen Ekstraksiyonu ve Karakterizasyonu. Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries, Cilt: 6 Sayı: 2, 166–177. https://doi.org/10.46384/jmsf.1387324

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Kaynakça (47)

  1. Abinaya, M., & Gayathri, M. (2019). Biodegradable collagen from Scomberomorus lineolatus skin for wound healing dressings and its application on antibiofilm properties. Journal of Cellular Biochemistry, 120, 15572-15584. doi: 10.1002/jcb.28824
  2. Ali, A. M. M., Benjakul, S., & Kishimura, H. (2017). Molecular characteristics of acid and pepsin soluble collagens from the scales of golden carp (Probarbus jullieni). Emirates Journal of Food and Agriculture, 29(6), 450-457. doi: 10.9755/ejfa.2016-09-1316
  3. AOAC (2002). Ash content. In Official Methods of Analysis, (17th Ed.) Association of Official Analytical Chemists, Method 920.153, Gaithersburg, Maryland.
  4. AOAC (2006a). Crude protein in meat. In official methods of analysis (17th ed.) 984.13. Gaithersburg, Association of Official Analytical Chemists.
  5. AOAC (2006b). Crude fat determination-soxhlet method. Meat technology information sheet, pp. 1-3. Ashraf, S. S., Adnan, M., Patel, M., Siddiqui, A. J., Sachidanandan, M., Snoussi, M., & Hadi, S. (2020). Fish-based bioactives as potent nutraceuticals: Exploring the therapeutic perspective of sustainable food from the sea. Marine Drugs, 18(265), 1-20. doi: 10.3390/md18050265
  6. Atef, M., & Ojagh, S. M. (2017). Health benefits and food applications of bioactive compounds from fish byproducts: A review. Journal of Functional Foods, 35, 673-681. doi: 10.1016/j.jff.2017.06.034
  7. Bhagwat, P. K., & Dandge, P. B. (2016). Isolation, characterization and valorizable applications of fish scale collagen in food and agriculture industries. Biocatalysis and Agricultural Biotechnology, 7, 234-240. doi: 10.1016/j.bcab.2016.06.010
  8. Bhuimbar, M. V., Bhagwat, P. K., & Dandge, P. B. (2019). Extraction and characterization of acid soluble collagen from fish waste: Development of collagen-chitosan blend as food packaging film. Journal of Environmental Chemical Engineering, 7, 102983. doi: 10.1016/j.jece.2019.102983
  9. Brand-Williams, W., Cuvelier, M. E., & Berset, C. L. W. T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28(1), 25-30. doi: 10.1016/S0023-6438(95)80008-5
  10. Bu, Y., Elango, J., Zhang, J., Bao, B., Guo, R., Palaniyandi, K., Robinson, J. S., Geevaretnam, J., Regenstein, J. M., & Wu, W. (2017). Immunological effects of collagen and collagen peptide from blue shark cartilage on 6T-CEM cells. Process Biochemistry, 57, 219-227. doi: 10.1016/j.procbio.2017.04.008
  11. Cao, T. H., Nguyen, T. T. O., Nguyen, T. M. H., Le, N. T., & Razumovskaya, R. G. (2017). Characteristics and physicochemical properties of gelatin extracted from scales of seabass (Lates calcarifer) and grey mullet (Mugil cephalus) in Vietnam. Journal of Aquatic Food Product Technology, 26(10), 1293-1302. doi: 10.1080/10498850.2017.1390026
  12. Chen, J., Li, L., Yi, R., Xu, N., Gao, R., & Hong, B. (2016). Extraction and characterization of acid-soluble collagen from scales and skin of tilapia (Oreochromis niloticus). LWT - Food Science and Technology, 66, 453-459. doi: 10.1016/j.lwt.2015.10.070
  13. Chen, S., Chen, H., Xie, Q., Hong, B., Chen, J., Hua, F., Bai, K., He, J., Yi, R., & Wu, H. (2016). Rapid isolation of high purity pepsin-soluble type I collagen from scales of red drum fish (Sciaenops ocellatus). Food Hydrocolloids, 52, 468-477. doi: 10.1016/j.foodhyd.2015.07.027
  14. Chen, Y., Liang, C., Wu, H., Pang, H., Chen, C., Wang, G., & Chan, L. (2018). Antioxidant and anti-inflammatory capacities of collagen peptides from milkfish (Chanos chanos) scales. Journal of Food Science and Technology, 55, 2310-2317. doi: 10.1007/s13197-018-3148-4
  15. Chinh, N. T., Manh, V. Q., Trung, V. Q., Lam, T. D., Huynh, M. D., Tung, N. Q., Trinh, N. D., & Hoang, T. (2019a). Characterization of collagen derived from tropical freshwater carp fish scale wastes and its amino acid sequence. Natural Product Communications, 1-12. doi: 10.1177/1934578X19866288
  16. Chinh, N. T., Manh, V. Q., Trung, V. Q., Trang, T. D. M., & Hoang, T. (2019b). Extraction of hydroxyapatite and collagen from the Vietnamese tilapia scales. Vietnam Journal of Chemistry, 57(2), 225-228. doi: 10.1002/vjch.201900021
  17. Chuaychan, S., Benjakul, S., & Kishimura, H. (2015). Characteristics of acid- and pepsin-soluble collagens from scale of seabass (Lates calcarifer). LWT - Food Science and Technology, 63, 71-76. doi: 10.1016/j.lwt.2015.03.002
  18. El-Rashidy, A. A., Gad, A., Abu-Hussein, A. E. G., Habib, S. I., Badr, N. A., & Hashem, A. A. (2015). Chemical and biological evaluation of Egyptian Nile Tilapia (Oreochromis niloticas) fish scale collagen. International Journal of Biological Macromolecules, 79, 618-626. doi: 10.1016/j.ijbiomac.2015.05.019
  19. Erkan, N., Selçuk, A., & Özden, Ö. (2010). Amino acid and vitamin composition of raw and cooked horse mackerel. Food Analytical Methods, 3, 269-275. doi: 10.1007/s12161-009-9108-x
  20. Fehng, S. P. C. (2016). Extraction of collagen from fish wastes, optimization and characterization. (M.Sc. Dissertation), Universiti Tunku Abdul Rahman, Kampar.
  21. Günlü, A., Sipahioğlu, S., & Alpas, H. (2014). The effect of high hydrostatic pressure on the muscle proteins of rainbow trout (Oncorhynchus mykiss Walbaum) fillets wrapped with chitosan-based edible film during cold storage (4±1 °C). High Pressure Research, 34(1), 122-132. doi: 10.1080/08957959.2014.894510
  22. Hamdi, M., Hammami, A., Hajji, S., Jridi, M., Nasri, M., & Nasri, M. (2017). Chitin extraction from blue crab (Portunus segnis) and shrimp (Penaeus kerathurus) shells using digestive alkaline proteases from P. segnis viscera. International Journal of Biological Macromolecules, 101, 455-463. doi: 10.1016/j.ijbiomac.2017.02.103
  23. Iswariya, S., Velswamy, P., & Uma T. S. (2018). Isolation and characterization of biocompatible collagen from the skin of puffer fish (Lagocephalus inermis). Journal of Polymers and the Environment, 26, 2086-2095. doi: 10.1007/s10924-017-1107-1
  24. Jafari, H., Lista, A., Siekapen, M. M., Ghaffari-Bohlouli, P., Nie, L., Alimoradi, H., & Shavandi, A. (2020). Fish Collagen: Extraction, characterization, and applications for biomaterials engineering. Polymers, 12(2230), 1-37. doi: 10.3390/polym12102230
  25. Knidri, H. E., Belaabed, R., Addaou, A., Laajeb, A., & Lahsini, A. (2018). Extraction, chemical modification and characterization of chitin and chitosan. International Journal of Biological Macromolecules, 120, 1181-1189. doi: 10.1016/j.ijbiomac.2018.08.139
  26. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
  27. Mensor, L. L., Menezes, F. S., Leitão, G. G., Reis, A. S., Santos, T. C. D., Coube, C. S., & Leitão, S. G. (2001). Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy Research, 15(2), 127-130. doi: 10.1002/ptr.687
  28. Nagai, T., & Suzuki, N. (2000). Isolation of collagen from fish waste material-skin, bone and fins. Food Chemistry, 68, 277-281. doi: 10.1016/S0308-8146(99)00188-0
  29. Nakchum, L., & Kim, S. M. (2016). Preparation of squid skin collagen hydrolysate as an antihyaluronidase, antityrosinase, and antioxidant agent. Preparative Biochemistry & Biotechnology, 46(2), 123-130. doi: 10.1080/10826068.2014.995808
  30. NCCLS (1993). Performance standards for antimicrobial disc suspectibility tests. Approved Standard NCCLS Publication M2-A5, Villanova, PA, USA.
  31. Nurilmala, M., Pertiwi, R. M., Nurhayati, T., Fauzi, S., Batubara, I., & Ochiai, Y. (2019). Characterization of collagen and its hydrolysate from yellowfin tuna Thunnus albacares skin and their potencies as antioxidant and antiglycation agents. Fisheries Science, 85, 591-599. doi: 10.1007/s12562-019-01303-5
  32. Pal, G. K., & Suresh, P. V. (2017). Comparative assessment of physico-chemical characteristics and fibril formation capacity of thermostable carp scales collagen. Materials Science and Engineering: C, 70, 32-40. doi: 10.1016/j.msec.2016.08.047
  33. Pal, P., Srivas, P. K., Dadhich, P., Das, B., Maity, P. P., Moulik, D., & Dhara, S. (2016). Accelerating full thickness wound healing using collagen sponge of mrigal fish (Cirrhinus cirrhosus) scale origin. International Journal of Biological Macromolecules, 93, 1507-1518. doi: 10.1016/j.ijbiomac.2016.04.032
  34. Pati, F., Adhikari, B., & Dhara, S. (2010). Isolation and characterization of fish scale collagen of higher thermal stability. Bioresource Technology, 101, 3737-3742. doi: 10.1016/j.biortech.2009.12.133
  35. Rajabimashhadi, Z., Gallo, N., Salvatore, L., & Lionetto, F. (2023). Collagen derived from fish industry waste: Progresses and challenges. Polymers, 15(3), 544. doi: 10.3390/polym15030544
  36. Shalaby, M., Agwa, M., Saeed, H., Khedr, S. M., Morsy, O., & El‑Demellawy, M. A. (2020). Fish scale collagen preparation, characterization and its application in wound healing. Journal of Polymers and the Environment, 28, 166-178. doi: 10.1007/s10924-019-01594-w
  37. Sionkowska, A., Kozlowska, J., Skorupska, M., & Michalska, M. (2015). Isolation and characterization of collagen from the skin of Brama australis. International Journal of Biological Macromolecules, 80, 605-609. doi: 10.1016/j.ijbiomac.2015.07.032
  38. Slimane, E. B., & Sadok, S. (2018). Collagen from cartilaginous fish by-products for a potential application in bioactive film composite. Marine Drugs, 16, 1-19. doi: 10.3390/md16060211
  39. Subhan, F., Hussain, Z., Tauseef, I., Shehzad, A., & Wahid, F. (2021). A review on recent advances and applications of fish collagen. Critical Reviews in Food Science and Nutrition, 61(6), 1024-1037. doi: 10.1080/10408398.2020.1751585
  40. Tan, Y., & Chang, S. K. C. (2018). Isolation and characterization of collagen extracted from channel catfish (Ictalurus punctatus) skin. Food Chemistry, 242, 147-155. doi: 10.1016/j.foodchem.2017.09.013
  41. Tang, C., Zhou, K., Zhu, Y., Zhang, W., Xie, Y., Wang, Z., Zhou, H., Yang, T., Zhang, Q., & Xu, B. (2022). Collagen and its derivatives: From structure and properties to their applications in food industry. Food Hydrocolloids, 131, 107748. doi: 10.1016/j.foodhyd.2022.107748
  42. Thuy, L. T. M., Okazaki, E., & Osako, K. (2014). Isolation and characterization of acid-soluble collagen from the scales of marine fishes from Japan and Vietnam. Food Chemistry, 149, 264-270. DOI: 10.1016/j.foodchem.2013.10.094
  43. Venkatesan, J., Anil, S., Kim, S., & Shim, M. S. (2017). Marine fish proteins and peptides for cosmeceuticals: A Review. Marine Drugs, 15, 1-18. doi: 10.3390/md15050143
  44. Wang, L., An, X., Yang, F., Xin, Z., & Zhao, L. (2008). Isolation and characterisation of collagens from the skin, scale and bone of deep-sea redfish (Sebastes mentella). Food Chemistry, 108, 616-623. doi: 10.1016/j.foodchem.2007.11.017
  45. Woo, J., Yu, S., Cho, S., Lee, Y., & Kim, S. (2008). Extraction optimization and properties of collagen from yellowfin tuna (Thunnus albacares) dorsal skin. Food Hydrocolloids, 22, 879-887. doi: 10.1016/j.foodhyd.2007.04.015
  46. Yu, P., & Gu, H. (2015). Bioactive substances from marine fishes, shrimps, and algae and their functions: Present and future. Critical Reviews in Food Science and Nutrition, 55, 1114-1136. doi: 10.1080/10408398.2012.686933
  47. Zayed, A. E. A. F. (2018). Bioactive compounds from marine sources. (M.Sc. Dissertation), Technischen Universität Kaiserslautern, Kaiserslautern.