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Dergiler / Hacettepe Journal of Biology and Chemistry / 2026 / Volume: 39 Issue: 2

Bazı Alfa-Karbonik Anhidrazlarda Oksitetrasiklinin Aktivatör Etkileri

Activatory Effects of Oxytetracycline on Some α-Carbonic Anhydrases

Ramazan Demirdağ(Atatürk University, Faculty of Science, Department of Chemistry, Erzurum, Turkey)
Murat Şentürk(Ağrı İbrahim Çeçen University, Faculty of Art and Science, Department of Chemistry, Ağrı, Turkey)
Ömer İrfan Küfrevioğlu(Atatürk University, Faculty of Science, Department of Chemistry, Erzurum, Turkey)
Sayfa
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Yayın Tarihi
2011-04-01
DOI
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bu antibiyotik için % aktivite-[A] grafiği kullanılarak % aktivite değerleri belirlendi

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Üç alfa-karbonik anhidraz, insan karbonik anhidraz I, II hCA I, II ve koyun böbrek karbonik anhidraz sCA saflaştırıldı ve enzimlerle oksitetrasiklinin kinetik etkileşmeleri incelendi.
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Temel Bulgular & Veri:Saflaştırma prosedürü homojenat hazırlama ve Sepharose 4B-trizonsüllfanilamid afinite kramatografisinden oluştu.
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Sonuç & Katkı:Bu antibiyotik için % aktivite-[A] grafiği kullanılarak % aktivite değerleri belirlendi
🔬Tür:Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Carbonic anhydrase · Human · Sheep

Özet

Üç alfa-karbonik anhidraz, insan karbonik anhidraz I, II hCA I, II ve koyun böbrek karbonik anhidraz sCA saflaştırıldı ve enzimlerle oksitetrasiklinin kinetik etkileşmeleri incelendi. Saflaştırma prosedürü homojenat hazırlama ve Sepharose 4B-trizonsüllfanilamid afinite kramatografisinden oluştu. Oksitetrasiklin hCA I, II ve sCA enzim aktivitelerinde in vitro aktivatör etkisi gösterdi. Bu antibiyotik için % aktivite-[A] grafiği kullanılarak % aktivite değerleri belirlendi

Abstract

Three -carbonic anhydrases, including human carbonic anhydrase I and II hCA I, II and sheep kidney carbonic anhydrase sCA were purified and kinetic interactions between the enzymes and oxytetracycline were investigated. The purification procedure was composed of preparation of homogenate or hemolysate and affinity chromatography on Sepharose 4B-tyrosine-sulfanilamide. Oxytetracycline exhibited in vitro activatory effects on hCA I, II and sCA enzymes activity. Activity % values for this antibiotic were determined by plotting activity % vs. [A]

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Bu Makaleye Atıf Yap

Demirdağ, R., Şentürk, M., Küfrevioğlu, Ö. İ. (2011). Bazı Alfa-Karbonik Anhidrazlarda Oksitetrasiklinin Aktivatör Etkileri. Hacettepe Journal of Biology and Chemistry, Volume: 39 Issue: 2. https://ajindex.com/articles/1271057

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Kaynakça (19)

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