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Dergiler / Journal of Istanbul Veterinary Sciences / 2025 / Cilt: 9 Sayı: 3

Molecular sex determination from feather-derived DNA in White storks (Ciconia ciconia) and Lovebirds (Agapornis spp.) using CHD-W and CHD-Z gene regions

İrem Özkan Kılıç(ISTANBUL UNIVERSITY-CERRAHPASA, INSTITUTE OF GRADUATE PROGRAMS)0009-0001-2592-7377
Kozet Avanus(İSTANBUL ÜNİVERSİTESİ-CERRAHPAŞA, VETERİNER FAKÜLTESİ, ZOOTEKNİ VE HAYVAN BESLEME BÖLÜMÜ, VETERİNERLİK ZOOTEKNİ ANABİLİM DALI)0000-0001-9886-7515
Ebuderda Günay(İSTANBUL ÜNİVERSİTESİ-CERRAHPAŞA, VETERİNER FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, YABANİ HAYVAN HASTALIKLARI VE EKOLOJİ ANABİLİM DALI)0000-0002-9767-7469
Ozan Gündemir(İSTANBUL ÜNİVERSİTESİ-CERRAHPAŞA, VETERİNER FAKÜLTESİ, VETERİNER HEKİMLİĞİ TEMEL BİLİMLERİ BÖLÜMÜ, VETERİNERLİK ANATOMİSİ ANABİLİM DALI)0000-0002-3637-8166
Sayfa
212–214
Yayın Tarihi
2025-12-31
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

These findings highlight the practical value of CHD-PCR for both conservation-focused studies of migratory species and routine management of captive psittacines.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Reliable sex determination is essential in avian research, conservation programs, and breeding management, particularly for species that lack visible sexual dimorphism.
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Temel Bulgular & Veri:This study evaluated the performance of CHD-based PCR sexing in two biologically distinct bird groups: wild white storks (Ciconia ciconia) undergoing migration and captive Lovebirds (Agapornis spp.).
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Sonuç & Katkı:These findings highlight the practical value of CHD-PCR for both conservation-focused studies of migratory species and routine management of captive psittacines.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:CHD-W · CHD-Z · White Storks

Özet

Reliable sex determination is essential in avian research, conservation programs, and breeding management, particularly for species that lack visible sexual dimorphism. This study evaluated the performance of CHD-based PCR sexing in two biologically distinct bird groups: wild white storks (Ciconia ciconia) undergoing migration and captive Lovebirds (Agapornis spp.). Feather samples were collected non-invasively from 15 white storks admitted to the Department of Wildlife Diseases and Ecology during the migration season, and from five Lovebirds; liver samples from an additional five Lovebirds that had died naturally were also analyzed to serve as an independent tissue source for confirmation of feather-based results. DNA extracted from feather calamus segments (4-6 cm in storks; 3-5 mm in Lovebirds) yielded consistent amplification of CHD-W and CHD-Z regions using the 2550F/2718R primer pair. Among storks, 11 individuals were identified as females and four as males. In Lovebirds, three were male and two female. The method produced clear, reproducible results across both species, confirming that feather-derived DNA provides a dependable source for molecular sexing. These findings highlight the practical value of CHD-PCR for both conservation-focused studies of migratory species and routine management of captive psittacines.

Abstract

Reliable sex determination is essential in avian research, conservation programs, and breeding management, particularly for species that lack visible sexual dimorphism. This study evaluated the performance of CHD-based PCR sexing in two biologically distinct bird groups: wild white storks (Ciconia ciconia) undergoing migration and captive Lovebirds (Agapornis spp.). Feather samples were collected non-invasively from 15 white storks admitted to the Department of Wildlife Diseases and Ecology during the migration season, and from five Lovebirds; liver samples from an additional five Lovebirds that had died naturally were also analyzed to serve as an independent tissue source for confirmation of feather-based results. DNA extracted from feather calamus segments (4-6 cm in storks; 3-5 mm in Lovebirds) yielded consistent amplification of CHD-W and CHD-Z regions using the 2550F/2718R primer pair. Among storks, 11 individuals were identified as females and four as males. In Lovebirds, three were male and two female. The method produced clear, reproducible results across both species, confirming that feather-derived DNA provides a dependable source for molecular sexing. These findings highlight the practical value of CHD-PCR for both conservation-focused studies of migratory species and routine management of captive psittacines.

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Bu Makaleye Atıf Yap

Kılıç, İ. Ö., Avanus, K., Günay, E., Gündemir, O. (2025). Molecular sex determination from feather-derived DNA in White storks (Ciconia ciconia) and Lovebirds (Agapornis spp.) using CHD-W and CHD-Z gene regions. Journal of Istanbul Veterinary Sciences, Cilt: 9 Sayı: 3, 212–214. https://doi.org/10.30704/http-www-jivs-net.1841187

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Kaynakça (11)

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