KaydedilenlerDerginizi ekleyin

Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2009 / Cilt: 33 - Sayı: 5

Effects of addition of vitamin B12 to the extender on post-thaw motility, acrosome morphology, and plasma membrane integrity in bull semen

Jian-hong HU
Qing-wang LI
Yu-lin CHEN
Zhong-liang JIANG
Yong-hong JIA
Sayfa
379–384
Yayın Tarihi
2009-05-01
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

In conclusion, the addition of 2.50 mg/mL vitamin B12 to the extender improved the bovine frozen semen quality.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:To investigate the effects of supplementation of vitamin B12 on bovine sperm post-thaw quality, vitamin B12 was added at the concentrations of 1.25, 2.50, 3.75, and 5.00 mg/mL to bovine semen cryoprotective medium.
📊
Temel Bulgular & Veri:The results indicated that the motility and VSL, VCL, STR, and VAP values of sperm supplemented with 2.50 mg/mL vitamin B12 were significantly higher than those of other concentrations (P < 0.05).
💡
Sonuç & Katkı:In conclusion, the addition of 2.50 mg/mL vitamin B12 to the extender improved the bovine frozen semen quality.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:vitamin B12 · cryopreservation · sperm motility

Özet

To investigate the effects of supplementation of vitamin B12 on bovine sperm post-thaw quality, vitamin B12 was added at the concentrations of 1.25, 2.50, 3.75, and 5.00 mg/mL to bovine semen cryoprotective medium. The results indicated that the motility and VSL, VCL, STR, and VAP values of sperm supplemented with 2.50 mg/mL vitamin B12 were significantly higher than those of other concentrations (P < 0.05). No significant difference was observed for LIN and ALH values and the percentage of grade a spermatozoa between the extenders containing 2.50 mg/mL and 3.75 mg/mL vitamin B12 (P > 0.05). The percentages of acrosome-intact and plasma membrane-intact spermatozoa were significantly improved (P < 0.05) by supplementing with 2.50 mg/mL vitamin B12. With all parameters measured, the concentration of 2.50 mg/mL vitamin B12 showed the best results on the quality of bovine semen in freezing-thawing. In conclusion, the addition of 2.50 mg/mL vitamin B12 to the extender improved the bovine frozen semen quality.

Abstract

To investigate the effects of supplementation of vitamin B12 on bovine sperm post-thaw quality, vitamin B12 was added at the concentrations of 1.25, 2.50, 3.75, and 5.00 mg/mL to bovine semen cryoprotective medium. The results indicated that the motility and VSL, VCL, STR, and VAP values of sperm supplemented with 2.50 mg/mL vitamin B12 were significantly higher than those of other concentrations (P < 0.05). No significant difference was observed for LIN and ALH values and the percentage of grade a spermatozoa between the extenders containing 2.50 mg/mL and 3.75 mg/mL vitamin B12 (P > 0.05). The percentages of acrosome-intact and plasma membrane-intact spermatozoa were significantly improved (P < 0.05) by supplementing with 2.50 mg/mL vitamin B12. With all parameters measured, the concentration of 2.50 mg/mL vitamin B12 showed the best results on the quality of bovine semen in freezing-thawing. In conclusion, the addition of 2.50 mg/mL vitamin B12 to the extender improved the bovine frozen semen quality.

🔖

Bu Makaleye Atıf Yap

HU, J., LI, Q., CHEN, Y., JIANG, Z., JIA, Y. (2009). Effects of addition of vitamin B12 to the extender on post-thaw motility, acrosome morphology, and plasma membrane integrity in bull semen. Turkish Journal of Veterinary and Animal Sciences, Cilt: 33 - Sayı: 5, 379–384. https://ajindex.com/articles/121992

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (9)

  1. Aboagla, E.M., Terada, T.: Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biol. Reprod., 2003; 69: 1245-1250.
  2. Sharp, A.A., Witts, L.J.: Seminal vitamin B12and sterility. Lancet, 1962; 1: 779. Pardanani, D.S., Kher, Y.R., Mahendrakar, M.N., Kirtane, S.T., Jayatilak, P.G.: Effects of testosterone-vitamin B12(Aquaviron B12) therapy in cases of oligospermia. Indian J. Surg., 1972; 34: 89-96.
  3. Halim, A., Antoniou, D., Leedham, P.W., Blandy, J.P., Tresidder, G.C.: Investigation and treatment of the infertile male. Proc. R. Soc. Med., 1973; 66: 373-378.
  4. Hirwe, R., Jathar, V.S., Desai, S., Satoskar, R.S.: Vitamin B12and potential fertility in male lactovegetarians. J. Biosoc. Sci., 1976; 8: 221-227.
  5. Jeyendran, R.S., Van der Ven, H.H., Perez-Pelaez, M., Crabo, B.G., Zaneveld, L.J.D.: Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil., 1984; 70: 219-228.
  6. Bergman, E.N., Roe, W.E., Kon, K.: Quantitative aspects of proprionate metabolism and gluconeogenesis in sheep. Am. J. Physiol., 1966; 211: 793-799.
  7. Kennedy, D.G., Cannavan, A., Molloy, A., O’Harte, F., Taylor, S.M., Kennedy, S., Blanchflower, W.J.: Methylmalonyl-CoA mutase (EC 5.4.99.2) and methionine synthetase (EC 2.1.1.13) in the tissues of cobalt-vitamin B12deficient sheep. Br. J. Nutr., 1990; 64: 721-732.
  8. Shangari, N., Bruce, W.R., Poon, R., O’Brien, P.J.: Toxicity of glyoxals-role of oxidative stress, metabolic detoxification and thiamine deficiency. Biochem. Soc. Trans., 2003; 31: 1390-1393. Vézina, D., Mauffette, F., Roberts, K.D., Bleau, G.: Selenium- vitamin E supplementation in infertile men. Effects on semen parameters and micronutrient levels and distribution. Biol. Trace Elem. Res., 1996; 53: 65-83.
  9. Alvarez, J.G., Storey, B.T.: Assessment of cell damage caused by spontaneous lipid peroxidation in rabbit spermatozoa. Biol. Reprod., 1984; 30: 323-331.