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Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2011 / Cilt: 35 - Sayı: 2

Disodium EDTA used as anticoagulant causes hemolysis in common carp blood

Malgorzata WITESKA*
Wioleta WARGOCKA
Sayfa
99–104
Yayın Tarihi
2011-02-01
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The obtained results revealed that Na2EDTA caused gradual damage to erythrocytes, and thus should not be applied as anticoagulant for blood analysis of common carp.

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Amaç & Hipotez:Disodium EDTA used as anticoagulant for common carp blood caused a significant increase and high variability in hematocrit readings comparing to the heparinized samples.
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Temel Bulgular & Veri:Na2EDTA induced erythrocyte swelling, causing cell membrane disruption (hemolysis).
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Sonuç & Katkı:The obtained results revealed that Na2EDTA caused gradual damage to erythrocytes, and thus should not be applied as anticoagulant for blood analysis of common carp.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Key words: Fish · erythrocytes · hematocrit

Özet

Disodium EDTA used as anticoagulant for common carp blood caused a significant increase and high variability in hematocrit readings comparing to the heparinized samples. Na2EDTA induced erythrocyte swelling, causing cell membrane disruption (hemolysis). The nuclei released from destroyed cells changed shape from oval to round, and underwent gradual swelling and vacuolation, followed by karyolysis. The degree of observed changes was similar at all Na2EDTA concentrations (0.1, 0.5, and 1.0 mg/mL); on the other hand, percentage of abnormal and destroyed cells showed considerable differences among blood sampled from various carp individuals. The obtained results revealed that Na2EDTA caused gradual damage to erythrocytes, and thus should not be applied as anticoagulant for blood analysis of common carp.

Abstract

Disodium EDTA used as anticoagulant for common carp blood caused a significant increase and high variability in hematocrit readings comparing to the heparinized samples. Na2EDTA induced erythrocyte swelling, causing cell membrane disruption (hemolysis). The nuclei released from destroyed cells changed shape from oval to round, and underwent gradual swelling and vacuolation, followed by karyolysis. The degree of observed changes was similar at all Na2EDTA concentrations (0.1, 0.5, and 1.0 mg/mL); on the other hand, percentage of abnormal and destroyed cells showed considerable differences among blood sampled from various carp individuals. The obtained results revealed that Na2EDTA caused gradual damage to erythrocytes, and thus should not be applied as anticoagulant for blood analysis of common carp.

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Bu Makaleye Atıf Yap

WITESKA*, M., WARGOCKA, W. (2011). Disodium EDTA used as anticoagulant causes hemolysis in common carp blood. Turkish Journal of Veterinary and Animal Sciences, Cilt: 35 - Sayı: 2, 99–104. https://ajindex.com/articles/121589

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Kaynakça (9)

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