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Journals / Turkish Journal of Veterinary and Animal Sciences / 2011 / Cilt: 35 - Sayı: 2

Disodium EDTA used as anticoagulant causes hemolysis in common carp blood

Malgorzata WITESKA*
Wioleta WARGOCKA
Pages
99–104
Publication Date
2011-02-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The obtained results revealed that Na2EDTA caused gradual damage to erythrocytes, and thus should not be applied as anticoagulant for blood analysis of common carp.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Disodium EDTA used as anticoagulant for common carp blood caused a significant increase and high variability in hematocrit readings comparing to the heparinized samples.
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Key Findings & Data:Na2EDTA induced erythrocyte swelling, causing cell membrane disruption (hemolysis).
💡
Conclusion & Impact:The obtained results revealed that Na2EDTA caused gradual damage to erythrocytes, and thus should not be applied as anticoagulant for blood analysis of common carp.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Fish · erythrocytes · hematocrit

Abstract

Disodium EDTA used as anticoagulant for common carp blood caused a significant increase and high variability in hematocrit readings comparing to the heparinized samples. Na2EDTA induced erythrocyte swelling, causing cell membrane disruption (hemolysis). The nuclei released from destroyed cells changed shape from oval to round, and underwent gradual swelling and vacuolation, followed by karyolysis. The degree of observed changes was similar at all Na2EDTA concentrations (0.1, 0.5, and 1.0 mg/mL); on the other hand, percentage of abnormal and destroyed cells showed considerable differences among blood sampled from various carp individuals. The obtained results revealed that Na2EDTA caused gradual damage to erythrocytes, and thus should not be applied as anticoagulant for blood analysis of common carp.

Özet

Disodium EDTA used as anticoagulant for common carp blood caused a significant increase and high variability in hematocrit readings comparing to the heparinized samples. Na2EDTA induced erythrocyte swelling, causing cell membrane disruption (hemolysis). The nuclei released from destroyed cells changed shape from oval to round, and underwent gradual swelling and vacuolation, followed by karyolysis. The degree of observed changes was similar at all Na2EDTA concentrations (0.1, 0.5, and 1.0 mg/mL); on the other hand, percentage of abnormal and destroyed cells showed considerable differences among blood sampled from various carp individuals. The obtained results revealed that Na2EDTA caused gradual damage to erythrocytes, and thus should not be applied as anticoagulant for blood analysis of common carp.

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Cite This Article

WITESKA*, M., WARGOCKA, W. (2011). Disodium EDTA used as anticoagulant causes hemolysis in common carp blood. Turkish Journal of Veterinary and Animal Sciences, Cilt: 35 - Sayı: 2, 99–104. https://ajindex.com/articles/121589

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References (9)

  1. Mariańska, B., Fabijańska-Mitek, J., Windyga, J.: Laboratory hematological analyses. Manual for medicine students. Wyd. Lek. PZWL, Warszawa, 2003 (book in Polish). Mohri, M., Shakeri, H., Lotfollah, Z.S.: Eff ects of common anticoagulants (heparin, citrate and EDTA) on routine plasma biochemistry of cattle. Comp. Clin. Pathol., 2007; 16: 207-209. 3. Hattingh, J., Smith, E.M.: Anticoagulants for avian and reptilian blood: heparin and EDTA. Pfl ugers Arch., 1976; 363: 267-269. 4. Muro, J., Cuenca, R., Pastor, J., Vinas, L., Lavin S.: Eff ects of lithium heparin and tripotassium EDTA on hematologic values of Hermann’s tortoises (Testudo hermanni). J. Zoo Wildl. Med., 1998; 29: 40-44.
  2. Svobodova, Z., Pravda, D., Palackova, J.: Unifi ed methods of haematological examination of fi sh. Research Institute of Fish Culture and Hydrobiology, Vodnany. 1991.
  3. Hattingh, J.: Heparin and ethylenediamine tetra-acetate as anticoagulants for fi sh blood. Pfl ügers Arch., 1975; 355: 347- 352. Walencik, J., Witeska, M.: Th e eff ects of anticoagulants on hematological indices and blood cell morphology of common carp (Cyprinus carpio L.). Comp. Biochem. Physiol., 2007; 146 C: 331-335.
  4. Korcock, D.E., Houston, A.H., Gray, J.D.: Eff ects of sampling conditions on selected blood variables of rainbow trout, Salmo gairdneri Richardson. J. Fish Biol., 1988; 33: 319-330.
  5. Tavares-Dias, M., Silva Sandrim, E.F.: Infl uence of anticoagulants and blood storage on hematological values in tambaqui, Colossoma macropomum. Acta Sci., 1998; 20: 151- 155. Smit, G.L., Hattingh, J., Schoonbee, H.B.: Observations on some eff ects of disodiumethylenediamine tetra-acetate and heparin on fi sh blood. Comp. Biochem. Physiol., 1977; 57C: 35-38.
  6. Blaxhall, P.C.: Error in haematocrit value produced by inadequate concentration of ethylenediamine tetra-acetate. J. Fish Biol., 1973; 5: 767-769.
  7. Olsen, A.K., Bladbjerg, E.M., Jansen, A.L., Hansen, A.K.: Eff ect of pre-analytical handling on haematological variables in minipigs. Lab. Anim., 2001; 35: 147-152.
  8. Mafuvadze, B., Erlwanger, K.H.: Th e eff ect of EDTA, heparin and storage on the erythrocyte osmotic fragility, plasma osmolality and haematocrit of adult ostriches (Struthio camelus). Veterinarski Archiv, 2007; 77: 427-434.
  9. Morris, J.D., Fernandez, J.M., Chapa, A.M., Gentry, L.R., Th orn, K.E., Weick, T.M.: Eff ects of sample handling, processing, storage, and hemolysis on measurements of key energy metabolites in ovine blood. Small Ruminant Research, 2002; 43